Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica
Multidrug-resistant pathogens have prompted the use of lytic bacteriophages. An indigenous novel lytic bacteriophage against Salmonella enterica strains from environmental wastewater was isolated and characterized using phage survivability study, adsorption curve, one-step curve, optimal multiplicit...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes Reports |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/29933935.2025.2452485 |
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| author | Ebele Onuigbo Paul Akpa Anthony Attama Stephen Emencheta Emmanuel Eze Chinonye Obeta |
| author_facet | Ebele Onuigbo Paul Akpa Anthony Attama Stephen Emencheta Emmanuel Eze Chinonye Obeta |
| author_sort | Ebele Onuigbo |
| collection | DOAJ |
| description | Multidrug-resistant pathogens have prompted the use of lytic bacteriophages. An indigenous novel lytic bacteriophage against Salmonella enterica strains from environmental wastewater was isolated and characterized using phage survivability study, adsorption curve, one-step curve, optimal multiplicity of infection, and phage-killing assay. The Salmonella strains CP90 and CP23 isolated from the same source were biochemically and molecularly characterized. The Salmonella strains CP90 and CP23 had 96.24% and 97.18% pairwise identity respectively with S. enterica. Both were resistant to B-lactam Aminoglycosides, Penicillin, and Phenicol class of antibiotics. The phage performed better in an alkaline medium and below 50°C. About 80% of the phage had an adsorption rate of 12 min and a latent period of 20 min. About 55 PFU/cell of the phage was released during a single replication cycle, inhibiting bacteria growth for up to 5 h. The characterization of this indigenous phage suggests its therapeutic potential against multidrug-resistant Salmonella species. |
| format | Article |
| id | doaj-art-4b594b2640ad40baaae4281aedf2401b |
| institution | DOAJ |
| issn | 2993-3935 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes Reports |
| spelling | doaj-art-4b594b2640ad40baaae4281aedf2401b2025-08-20T03:05:25ZengTaylor & Francis GroupGut Microbes Reports2993-39352025-12-012110.1080/29933935.2025.2452485Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella entericaEbele Onuigbo0Paul Akpa1Anthony Attama2Stephen Emencheta3Emmanuel Eze4Chinonye Obeta5Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaMultidrug-resistant pathogens have prompted the use of lytic bacteriophages. An indigenous novel lytic bacteriophage against Salmonella enterica strains from environmental wastewater was isolated and characterized using phage survivability study, adsorption curve, one-step curve, optimal multiplicity of infection, and phage-killing assay. The Salmonella strains CP90 and CP23 isolated from the same source were biochemically and molecularly characterized. The Salmonella strains CP90 and CP23 had 96.24% and 97.18% pairwise identity respectively with S. enterica. Both were resistant to B-lactam Aminoglycosides, Penicillin, and Phenicol class of antibiotics. The phage performed better in an alkaline medium and below 50°C. About 80% of the phage had an adsorption rate of 12 min and a latent period of 20 min. About 55 PFU/cell of the phage was released during a single replication cycle, inhibiting bacteria growth for up to 5 h. The characterization of this indigenous phage suggests its therapeutic potential against multidrug-resistant Salmonella species.https://www.tandfonline.com/doi/10.1080/29933935.2025.2452485BacteriophageSalmonella entericalyticnativetherapy |
| spellingShingle | Ebele Onuigbo Paul Akpa Anthony Attama Stephen Emencheta Emmanuel Eze Chinonye Obeta Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica Gut Microbes Reports Bacteriophage Salmonella enterica lytic native therapy |
| title | Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica |
| title_full | Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica |
| title_fullStr | Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica |
| title_full_unstemmed | Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica |
| title_short | Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica |
| title_sort | characterization of an indigenous lytic phage targeting multidrug resistant salmonella enterica |
| topic | Bacteriophage Salmonella enterica lytic native therapy |
| url | https://www.tandfonline.com/doi/10.1080/29933935.2025.2452485 |
| work_keys_str_mv | AT ebeleonuigbo characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica AT paulakpa characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica AT anthonyattama characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica AT stephenemencheta characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica AT emmanueleze characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica AT chinonyeobeta characterizationofanindigenouslyticphagetargetingmultidrugresistantsalmonellaenterica |