Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway

Fine particulate matter (PM2.5) remains a major environmental problem both in China and worldwide. Extensive researches have indicated that PM2.5 exposure can lead to various adverse health effects through pulmonary and systemic inflammation, making it crucial to explore effective individual interve...

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Main Authors: Yuanting Xie, Jing Han, Qianfeng Wang, Xinyi Fang, Bin Pan, Dongyang Han, Jingyu Li, Wenpu Shao, Xiaoqing Hong, Wenyue Tu, Wenye Geng, Weihua Li, Zhanzhuang Tian, Renjie Chen, Yanyi Xu, Haidong Kan
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325005202
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author Yuanting Xie
Jing Han
Qianfeng Wang
Xinyi Fang
Bin Pan
Dongyang Han
Jingyu Li
Wenpu Shao
Xiaoqing Hong
Wenyue Tu
Wenye Geng
Weihua Li
Zhanzhuang Tian
Renjie Chen
Yanyi Xu
Haidong Kan
author_facet Yuanting Xie
Jing Han
Qianfeng Wang
Xinyi Fang
Bin Pan
Dongyang Han
Jingyu Li
Wenpu Shao
Xiaoqing Hong
Wenyue Tu
Wenye Geng
Weihua Li
Zhanzhuang Tian
Renjie Chen
Yanyi Xu
Haidong Kan
author_sort Yuanting Xie
collection DOAJ
description Fine particulate matter (PM2.5) remains a major environmental problem both in China and worldwide. Extensive researches have indicated that PM2.5 exposure can lead to various adverse health effects through pulmonary and systemic inflammation, making it crucial to explore effective individual intervention strategies. Electroacupuncture, an ancient Chinese medical treatment, has been proven safe and effective for treating some diseases, however, its potential in preventing PM2.5-induced toxicity remains unclear. This study aimed to explore the potential of electroacupuncture in mitigating pulmonary inflammation induced by diesel exhaust particles (DEP). Electroacupuncture was administered 15 minutes before intratracheal instillation of DEP, and the results showed that it markedly reduced DEP-induced pulmonary inflammation, as evidenced by significantly decreased pro-inflammatory markers at both gene and protein levels in lung, via regulating the macrophage polarization. Further analysis indicated that electroacupuncture promoted the production and release of dopamine from the adrenal medulla of mice, which then translocated to lung via circulation and inhibited the pulmonary NLRP3/caspase-1 signaling pathway. In addition, the time effectiveness experiment suggested that the anti-inflammatory effect of electroacupuncture against DEP can last for 48 hours. These findings suggest that electroacupuncture holds potential as a therapeutic intervention for health issues caused by PM2.5 exposure.
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series Ecotoxicology and Environmental Safety
spelling doaj-art-4b5881c390344fbf96c07ee9286e29d72025-08-20T02:18:51ZengElsevierEcotoxicology and Environmental Safety0147-65132025-05-0129611818410.1016/j.ecoenv.2025.118184Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathwayYuanting Xie0Jing Han1Qianfeng Wang2Xinyi Fang3Bin Pan4Dongyang Han5Jingyu Li6Wenpu Shao7Xiaoqing Hong8Wenyue Tu9Wenye Geng10Weihua Li11Zhanzhuang Tian12Renjie Chen13Yanyi Xu14Haidong Kan15School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaState Key Laboratory of Medical Neurobiology, Department of Integrative Medicine and Neurobiology, Brain Science Collaborative Innovation Center, School of Basic Medical Sciences, Institutes of Brain Science, Fudan Institutes of Integrative Medicine, Fudan University, Shanghai 200032, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaScientific Research Department of Shanghai Medical College, Fudan Zhangjiang Institute, Fudan University, Shanghai 200032, ChinaNHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Pharmacy, Fudan University, Shanghai 200032, ChinaState Key Laboratory of Medical Neurobiology, Department of Integrative Medicine and Neurobiology, Brain Science Collaborative Innovation Center, School of Basic Medical Sciences, Institutes of Brain Science, Fudan Institutes of Integrative Medicine, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, ChinaSchool of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, China; Correspondence to: School of Public Health, Fudan University, 130 Dong’an Rd, Shanghai 200032, China.School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, China; NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Pharmacy, Fudan University, Shanghai 200032, China; Correspondence to: School of Public Health, Fudan University, 130 Dong’an Rd, Shanghai 200032, China.Fine particulate matter (PM2.5) remains a major environmental problem both in China and worldwide. Extensive researches have indicated that PM2.5 exposure can lead to various adverse health effects through pulmonary and systemic inflammation, making it crucial to explore effective individual intervention strategies. Electroacupuncture, an ancient Chinese medical treatment, has been proven safe and effective for treating some diseases, however, its potential in preventing PM2.5-induced toxicity remains unclear. This study aimed to explore the potential of electroacupuncture in mitigating pulmonary inflammation induced by diesel exhaust particles (DEP). Electroacupuncture was administered 15 minutes before intratracheal instillation of DEP, and the results showed that it markedly reduced DEP-induced pulmonary inflammation, as evidenced by significantly decreased pro-inflammatory markers at both gene and protein levels in lung, via regulating the macrophage polarization. Further analysis indicated that electroacupuncture promoted the production and release of dopamine from the adrenal medulla of mice, which then translocated to lung via circulation and inhibited the pulmonary NLRP3/caspase-1 signaling pathway. In addition, the time effectiveness experiment suggested that the anti-inflammatory effect of electroacupuncture against DEP can last for 48 hours. These findings suggest that electroacupuncture holds potential as a therapeutic intervention for health issues caused by PM2.5 exposure.http://www.sciencedirect.com/science/article/pii/S0147651325005202Air pollutionDiesel exhaust particlesDopamineElectroacupunctureInflammatory response
spellingShingle Yuanting Xie
Jing Han
Qianfeng Wang
Xinyi Fang
Bin Pan
Dongyang Han
Jingyu Li
Wenpu Shao
Xiaoqing Hong
Wenyue Tu
Wenye Geng
Weihua Li
Zhanzhuang Tian
Renjie Chen
Yanyi Xu
Haidong Kan
Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
Ecotoxicology and Environmental Safety
Air pollution
Diesel exhaust particles
Dopamine
Electroacupuncture
Inflammatory response
title Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
title_full Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
title_fullStr Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
title_full_unstemmed Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
title_short Electroacupuncture alleviates diesel exhaust particles-induced inflammatory response in lung through dopamine inhibition of NLRP3 signaling pathway
title_sort electroacupuncture alleviates diesel exhaust particles induced inflammatory response in lung through dopamine inhibition of nlrp3 signaling pathway
topic Air pollution
Diesel exhaust particles
Dopamine
Electroacupuncture
Inflammatory response
url http://www.sciencedirect.com/science/article/pii/S0147651325005202
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