Establishment of knockout mutagenesis in avrBs3/pthA family genes of Xanthomonas oryzae pv. oryzicola
The contribution of at least twenty highly conserved avrBs3/pthA family genes in Xanthomonas oryz ae pv. oryzicola (Xoc) to virulence or avirulence in rice is still rudimentary. Based on the conserved structure of the avrBs3/pthA family, a marker-exchange mutagenesis strategy in association with med...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.01.006 |
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| Summary: | The contribution of at least twenty highly conserved avrBs3/pthA family genes in Xanthomonas oryz ae pv. oryzicola (Xoc) to virulence or avirulence in rice is still rudimentary. Based on the conserved structure of the avrBs3/pthA family, a marker-exchange mutagenesis strategy in association with mediation by a suicide vector pKMS1 was used to knock out the avrBs3/pthA family genes of Xoc. The results showed that total five avrBs3/pthA genes were gradually knocked out based on the conserved structure of the family by using marker-exchange mutagenesis mediated by a suicide vector pKMS1. The knock-out mutation occurred either within an avrBs3/pthA gene or between two av rBs3/pthA genes. PCR and Southern hybridization analysis demonstrated that the establishment of knock-out mutagenesis in avrBs3/pthA genes of Xoc was successful, providing a platform to assess virulence or/and avirulence roles contributed by each member of the avrBs3/pthA family in rice. |
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| ISSN: | 1008-9209 2097-5155 |