Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells

The present work aims to investigate and validate the medicinal potential of Acorus calamus, a plant known for its high therapeutic potential. The focus was identifying biologically active extracts and compounds that exhibit promising anticancer properties. The cytotoxicity of the extracts and compo...

Full description

Saved in:
Bibliographic Details
Main Authors: Sabiyah Akhter, Malik Muzafar Manzoor, Sameer Ahmad Mir, Tahirah Khaliq, Phalisteen Sultan, Qazi Parvaiz Hassan
Format: Article
Language:English
Published: Maximum Academic Press 2024-01-01
Series:Medicinal Plant Biology
Subjects:
Online Access:https://www.maxapress.com/article/doi/10.48130/mpb-0024-0021
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850148319870844928
author Sabiyah Akhter
Malik Muzafar Manzoor
Sameer Ahmad Mir
Tahirah Khaliq
Phalisteen Sultan
Qazi Parvaiz Hassan
author_facet Sabiyah Akhter
Malik Muzafar Manzoor
Sameer Ahmad Mir
Tahirah Khaliq
Phalisteen Sultan
Qazi Parvaiz Hassan
author_sort Sabiyah Akhter
collection DOAJ
description The present work aims to investigate and validate the medicinal potential of Acorus calamus, a plant known for its high therapeutic potential. The focus was identifying biologically active extracts and compounds that exhibit promising anticancer properties. The cytotoxicity of the extracts and compounds extracted from the rhizomes of A. calamus was assessed in three different cancer cell lines: lung carcinoma (A549), colon carcinoma (HCT-116), and breast carcinoma (MDA-MB-231). The application of extracts derived from the rhizome of A. calamus significantly inhibited cell proliferation in various cancer cell lines, including lung, colon, and breast cancer cells. Furthermore, the isolated compounds ACS08 (stearic acid), ACS06 (beta-sitosterol), and ACS02 (β-asarone) exhibited superior anticancer activity against all the tested cancer cell lines, with an inhibition rate of ≥ 50%. Compared with ACS06 and ACS02, ACS08 exhibited greater cytotoxicity. Among the panel of cancer cell lines utilized, compound ACS08 exhibited greater efficacy against the MDA-MB-231 breast cancer cell line, as evidenced by an IC50 estimation of 55.89 μM.
format Article
id doaj-art-4b3f9bc33ece45fba6c331fa3cd16cc6
institution OA Journals
issn 2835-6969
language English
publishDate 2024-01-01
publisher Maximum Academic Press
record_format Article
series Medicinal Plant Biology
spelling doaj-art-4b3f9bc33ece45fba6c331fa3cd16cc62025-08-20T02:27:18ZengMaximum Academic PressMedicinal Plant Biology2835-69692024-01-01311710.48130/mpb-0024-0021mpb-0024-0021Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cellsSabiyah Akhter0Malik Muzafar Manzoor1Sameer Ahmad Mir2Tahirah Khaliq3Phalisteen Sultan4Qazi Parvaiz Hassan5Plant Molecular Biology and Biotechnology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaPlant Molecular Biology and Biotechnology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaCancer Pharmacology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaPlant Molecular Biology and Biotechnology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaPlant Molecular Biology and Biotechnology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaPlant Molecular Biology and Biotechnology Division, CSIR Indian Institute of Integrative Medicine, Sanat Nagar-Srinagar 190005, Jammu and Kashmir, IndiaThe present work aims to investigate and validate the medicinal potential of Acorus calamus, a plant known for its high therapeutic potential. The focus was identifying biologically active extracts and compounds that exhibit promising anticancer properties. The cytotoxicity of the extracts and compounds extracted from the rhizomes of A. calamus was assessed in three different cancer cell lines: lung carcinoma (A549), colon carcinoma (HCT-116), and breast carcinoma (MDA-MB-231). The application of extracts derived from the rhizome of A. calamus significantly inhibited cell proliferation in various cancer cell lines, including lung, colon, and breast cancer cells. Furthermore, the isolated compounds ACS08 (stearic acid), ACS06 (beta-sitosterol), and ACS02 (β-asarone) exhibited superior anticancer activity against all the tested cancer cell lines, with an inhibition rate of ≥ 50%. Compared with ACS06 and ACS02, ACS08 exhibited greater cytotoxicity. Among the panel of cancer cell lines utilized, compound ACS08 exhibited greater efficacy against the MDA-MB-231 breast cancer cell line, as evidenced by an IC50 estimation of 55.89 μM.https://www.maxapress.com/article/doi/10.48130/mpb-0024-0021acorus calamuscompoundsanticancermtt assaymda-mb-231
spellingShingle Sabiyah Akhter
Malik Muzafar Manzoor
Sameer Ahmad Mir
Tahirah Khaliq
Phalisteen Sultan
Qazi Parvaiz Hassan
Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
Medicinal Plant Biology
acorus calamus
compounds
anticancer
mtt assay
mda-mb-231
title Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
title_full Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
title_fullStr Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
title_full_unstemmed Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
title_short Extracts and isolated compounds from the Acorus calamus L. (sweet flag) rhizome showed distinct antimetastatic activity against MDA-MB-231 breast cancer cells
title_sort extracts and isolated compounds from the acorus calamus l sweet flag rhizome showed distinct antimetastatic activity against mda mb 231 breast cancer cells
topic acorus calamus
compounds
anticancer
mtt assay
mda-mb-231
url https://www.maxapress.com/article/doi/10.48130/mpb-0024-0021
work_keys_str_mv AT sabiyahakhter extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells
AT malikmuzafarmanzoor extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells
AT sameerahmadmir extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells
AT tahirahkhaliq extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells
AT phalisteensultan extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells
AT qaziparvaizhassan extractsandisolatedcompoundsfromtheacoruscalamuslsweetflagrhizomeshoweddistinctantimetastaticactivityagainstmdamb231breastcancercells