Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection
Abstract Microtus fortis (M. fortis) is the only mammal known in China that is intrinsically resistant to Schistosoma japonicum (S. japonicum) infection. Nevertheless, the underlying resistance mechanism of M. fortis against schistosomes are still unclear. In this study, we detected and compared col...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-91164-z |
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| author | Tianqiong He Du Zhang Yixin Wen Qian Liu Junkang Zhou Wenling Zhi Lingxuan OuYang Yushan Qi Zikang Zhou Xin Gao Fan Li Zhijie Su Jia Shen Zhijun Zhou |
| author_facet | Tianqiong He Du Zhang Yixin Wen Qian Liu Junkang Zhou Wenling Zhi Lingxuan OuYang Yushan Qi Zikang Zhou Xin Gao Fan Li Zhijie Su Jia Shen Zhijun Zhou |
| author_sort | Tianqiong He |
| collection | DOAJ |
| description | Abstract Microtus fortis (M. fortis) is the only mammal known in China that is intrinsically resistant to Schistosoma japonicum (S. japonicum) infection. Nevertheless, the underlying resistance mechanism of M. fortis against schistosomes are still unclear. In this study, we detected and compared colon aqueous extracts and serum metabolic profiles between M. fortis and ICR mice before and after S. japonicum infection using liquid chromatography–mass spectrometry (LC–MS). We identified 232 specific colon aqueous extract metabolites and 79 specific serum metabolites of M. fortis infected with or without S. japonicum at two weeks compared with those of ICR mice, which might be closely correlated with the time-course of schistosomiasis progression and could also be used as indicators for the M. fortis against S. japonicum, for example, nonadecanoic acid, hesperetin, glycocholic acid, 2-Aminobenzoic acid, 6-hydroxydaidzein and spermidine. And the enriched pathways were further identified, our findings revealed that S. japonicum infection induced the metabolic changes involved in a variety of metabolic pathways including amino acid metabolism, lipid metabolism, ABC transporters, central carbon metabolism in cancer and bile secretion. These results indicated that the colon aqueous extracts and serum metabolic profiles were significantly different between M. fortis and ICR mice before and after S. japonicum infection and will provide new insights into the underlying resistance mechanism of M. fortis against S. japonicum infection and identify promising candidates for the use of drugs against schistosomes. |
| format | Article |
| id | doaj-art-4b3d694a58774e10a860f8a9cbed129c |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4b3d694a58774e10a860f8a9cbed129c2025-08-20T03:04:27ZengNature PortfolioScientific Reports2045-23222025-02-0115111510.1038/s41598-025-91164-zMetabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infectionTianqiong He0Du Zhang1Yixin Wen2Qian Liu3Junkang Zhou4Wenling Zhi5Lingxuan OuYang6Yushan Qi7Zikang Zhou8Xin Gao9Fan Li10Zhijie Su11Jia Shen12Zhijun Zhou13Department of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Medical Genetics, The Second Xiangya Hospital, Central South UniversityDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Laboratory Animal Science, Xiangya Medical CollegeDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen UniversityDepartment of Laboratory Animal Science, Xiangya Medical CollegeAbstract Microtus fortis (M. fortis) is the only mammal known in China that is intrinsically resistant to Schistosoma japonicum (S. japonicum) infection. Nevertheless, the underlying resistance mechanism of M. fortis against schistosomes are still unclear. In this study, we detected and compared colon aqueous extracts and serum metabolic profiles between M. fortis and ICR mice before and after S. japonicum infection using liquid chromatography–mass spectrometry (LC–MS). We identified 232 specific colon aqueous extract metabolites and 79 specific serum metabolites of M. fortis infected with or without S. japonicum at two weeks compared with those of ICR mice, which might be closely correlated with the time-course of schistosomiasis progression and could also be used as indicators for the M. fortis against S. japonicum, for example, nonadecanoic acid, hesperetin, glycocholic acid, 2-Aminobenzoic acid, 6-hydroxydaidzein and spermidine. And the enriched pathways were further identified, our findings revealed that S. japonicum infection induced the metabolic changes involved in a variety of metabolic pathways including amino acid metabolism, lipid metabolism, ABC transporters, central carbon metabolism in cancer and bile secretion. These results indicated that the colon aqueous extracts and serum metabolic profiles were significantly different between M. fortis and ICR mice before and after S. japonicum infection and will provide new insights into the underlying resistance mechanism of M. fortis against S. japonicum infection and identify promising candidates for the use of drugs against schistosomes.https://doi.org/10.1038/s41598-025-91164-zMetabolomicsSerumColon aqueous extractsSchistosoma japonicumM. fortisICR mice |
| spellingShingle | Tianqiong He Du Zhang Yixin Wen Qian Liu Junkang Zhou Wenling Zhi Lingxuan OuYang Yushan Qi Zikang Zhou Xin Gao Fan Li Zhijie Su Jia Shen Zhijun Zhou Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection Scientific Reports Metabolomics Serum Colon aqueous extracts Schistosoma japonicum M. fortis ICR mice |
| title | Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection |
| title_full | Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection |
| title_fullStr | Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection |
| title_full_unstemmed | Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection |
| title_short | Metabolomic analysis of the intrinsic resistance mechanisms of Microtus fortis against Schistosoma japonicum infection |
| title_sort | metabolomic analysis of the intrinsic resistance mechanisms of microtus fortis against schistosoma japonicum infection |
| topic | Metabolomics Serum Colon aqueous extracts Schistosoma japonicum M. fortis ICR mice |
| url | https://doi.org/10.1038/s41598-025-91164-z |
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