Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.)
Cinnamoyl-CoA reductase (CCR), a key rate-limiting enzyme in plant lignin biosynthesis, critically regulates plant growth, development, and abiotic stress responses. As one of the world’s four major staple crops, potato (Solanum tuberosum L.) is extensively cultivated due to its nutritional value an...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1638073/full |
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| author | Chong Du Yunzhu Che Zengli Zhang Fumeng He Xinqi Zhang Yujie Han Zelin Yang Jiaqi Wang Tianshuai Qi Ying Lan Yingnan Wang Fenglan Li |
| author_facet | Chong Du Yunzhu Che Zengli Zhang Fumeng He Xinqi Zhang Yujie Han Zelin Yang Jiaqi Wang Tianshuai Qi Ying Lan Yingnan Wang Fenglan Li |
| author_sort | Chong Du |
| collection | DOAJ |
| description | Cinnamoyl-CoA reductase (CCR), a key rate-limiting enzyme in plant lignin biosynthesis, critically regulates plant growth, development, and abiotic stress responses. As one of the world’s four major staple crops, potato (Solanum tuberosum L.) is extensively cultivated due to its nutritional value and versatile applications, underscoring the importance of investigating the StCCR gene family and its expression patterns under abiotic stress. In this study, we identified 10 CCR genes from the Atlantic potato genome and conducted comprehensive analyses of their phylogenetic relationships, gene structures, species collinearity, cis-regulatory elements, and expression specificity. Virus-induced gene silencing (VIGS) was used to silence StCCR6, resulting in altered lignin content and enhanced susceptibility to bacterial infection. Results revealed structural and functional divergences among StCCRs members. Tissue-specific expression profiling demonstrated higher transcript abundance in stems and leaves compared to roots. Notably, StCCRs exhibited differential expression patterns across multiple stress conditions, with Subfamily I genes showing consistent upregulation under various treatments, suggesting their potential as core candidates mediating stress-responsive lignification. Silencing of StCCR6 altered lignin content and cell wall structure in potato, and the oxidative damage was more serious after bacterial infection. These findings establish a foundation for elucidating the functional roles of StCCRs in potato growth regulation and stress adaptation mechanisms. |
| format | Article |
| id | doaj-art-4b2b6949addb46c5a35722cf26e189c2 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-4b2b6949addb46c5a35722cf26e189c22025-08-20T03:39:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.16380731638073Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.)Chong Du0Yunzhu Che1Zengli Zhang2Fumeng He3Xinqi Zhang4Yujie Han5Zelin Yang6Jiaqi Wang7Tianshuai Qi8Ying Lan9Yingnan Wang10Fenglan Li11College of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaQiqihar Branch, Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, ChinaCinnamoyl-CoA reductase (CCR), a key rate-limiting enzyme in plant lignin biosynthesis, critically regulates plant growth, development, and abiotic stress responses. As one of the world’s four major staple crops, potato (Solanum tuberosum L.) is extensively cultivated due to its nutritional value and versatile applications, underscoring the importance of investigating the StCCR gene family and its expression patterns under abiotic stress. In this study, we identified 10 CCR genes from the Atlantic potato genome and conducted comprehensive analyses of their phylogenetic relationships, gene structures, species collinearity, cis-regulatory elements, and expression specificity. Virus-induced gene silencing (VIGS) was used to silence StCCR6, resulting in altered lignin content and enhanced susceptibility to bacterial infection. Results revealed structural and functional divergences among StCCRs members. Tissue-specific expression profiling demonstrated higher transcript abundance in stems and leaves compared to roots. Notably, StCCRs exhibited differential expression patterns across multiple stress conditions, with Subfamily I genes showing consistent upregulation under various treatments, suggesting their potential as core candidates mediating stress-responsive lignification. Silencing of StCCR6 altered lignin content and cell wall structure in potato, and the oxidative damage was more serious after bacterial infection. These findings establish a foundation for elucidating the functional roles of StCCRs in potato growth regulation and stress adaptation mechanisms.https://www.frontiersin.org/articles/10.3389/fpls.2025.1638073/fullpotatogene familyCCRabiotic stressgene expressionVIGS |
| spellingShingle | Chong Du Yunzhu Che Zengli Zhang Fumeng He Xinqi Zhang Yujie Han Zelin Yang Jiaqi Wang Tianshuai Qi Ying Lan Yingnan Wang Fenglan Li Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) Frontiers in Plant Science potato gene family CCR abiotic stress gene expression VIGS |
| title | Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) |
| title_full | Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) |
| title_fullStr | Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) |
| title_full_unstemmed | Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) |
| title_short | Identification and expression analysis of cinnamyl CoA reductase gene family and function of StCCR6 in potato (Solanum tuberosum L.) |
| title_sort | identification and expression analysis of cinnamyl coa reductase gene family and function of stccr6 in potato solanum tuberosum l |
| topic | potato gene family CCR abiotic stress gene expression VIGS |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1638073/full |
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