A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies
A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylch...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/232659 |
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| author | Sofija Andjelic Xhevat Lumi Zoltán Veréb Natasha Josifovska Andrea Facskó Marko Hawlina Goran Petrovski |
| author_facet | Sofija Andjelic Xhevat Lumi Zoltán Veréb Natasha Josifovska Andrea Facskó Marko Hawlina Goran Petrovski |
| author_sort | Sofija Andjelic |
| collection | DOAJ |
| description | A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment. |
| format | Article |
| id | doaj-art-4b27bc0669ad49e5aae682b6d3597ab5 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4b27bc0669ad49e5aae682b6d3597ab52025-02-03T07:26:10ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/232659232659A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory StudiesSofija Andjelic0Xhevat Lumi1Zoltán Veréb2Natasha Josifovska3Andrea Facskó4Marko Hawlina5Goran Petrovski6Eye Hospital, University Medical Centre, 1000 Ljubljana, SloveniaEye Hospital, University Medical Centre, 1000 Ljubljana, SloveniaDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, HungaryDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, HungaryDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, HungaryEye Hospital, University Medical Centre, 1000 Ljubljana, SloveniaDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, HungaryA novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.http://dx.doi.org/10.1155/2014/232659 |
| spellingShingle | Sofija Andjelic Xhevat Lumi Zoltán Veréb Natasha Josifovska Andrea Facskó Marko Hawlina Goran Petrovski A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies Journal of Immunology Research |
| title | A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies |
| title_full | A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies |
| title_fullStr | A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies |
| title_full_unstemmed | A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies |
| title_short | A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies |
| title_sort | simple method for establishing adherent ex vivo explant cultures from human eye pathologies for use in subsequent calcium imaging and inflammatory studies |
| url | http://dx.doi.org/10.1155/2014/232659 |
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