An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan
Host genetic factors play a role in determining susceptibility to infectious diseases ofhumans. Polymorphisms in Interleukin 4 (IL-4), an anti-inflammatory cytokine thatregulates the balance between T helper (Th1) and Th2 immune responses, has beenreported to affect the risk of infectious and autoi...
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| Language: | English |
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Elsevier
2016-02-01
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| Series: | Kuwait Journal of Science |
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| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/59 |
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| author | Manal A. Fadl Mawada A. Aydarous Canguan Mao Afshan Yasmeen |
| author_facet | Manal A. Fadl Mawada A. Aydarous Canguan Mao Afshan Yasmeen |
| author_sort | Manal A. Fadl |
| collection | DOAJ |
| description | Host genetic factors play a role in determining susceptibility to infectious diseases ofhumans. Polymorphisms in Interleukin 4 (IL-4), an anti-inflammatory cytokine thatregulates the balance between T helper (Th1) and Th2 immune responses, has beenreported to affect the risk of infectious and autoimmune diseases.
In this study we aimed to investigate the possible association of variable numberof tandem repeat (VNTR) polymorphism in intron3 of IL-4 with susceptibility totyphoid fever in Sudanese patients. We analyzed the 70 bp intron-3 VNTR repeatpolymorphism in 200 DNA samples obtained from patients with blood cultureconfirmedtyphoid fever and 200 samples from randomly selected healthy controlsfrom the same city area. The intronic VNTR polymorphism was genotyped using PCR,followed by agarose gel elec trophoresis. The result showed that the genotypes of thetarget VNTR polymorphism in both cases and controls were not deviated from Hardy-Weinberg equilibrium (X 2, df=3=6.468, P˃ 0.05 and X 2, df=1=0.07, P˃ 0.5 respectively).The observed difference of the R3R3 & R2R2 genotypes among cases and controls isnot statistically significant (P= 0.09 and P=0.46 respectively). However, a significantincrease in the 2R3R genotype frequency among cases compared to the controls wasobserved (P˂0.05), indicating that the heterozygote 2R3R of VNTR polymorphism ofIL4 might affect individual susceptibility to typhoid fever in our population.
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| format | Article |
| id | doaj-art-4b0f8e9e06e64e3daeeb489ddaa0be95 |
| institution | OA Journals |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kuwait Journal of Science |
| spelling | doaj-art-4b0f8e9e06e64e3daeeb489ddaa0be952025-08-20T02:03:39ZengElsevierKuwait Journal of Science2307-41082307-41162016-02-0143119An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, SudanManal A. Fadl0Mawada A. Aydarous1Canguan MaoAfshan YasmeenFaculty of Science and Technology,Alneelain University/ sudanAlneelain University/ sudan Host genetic factors play a role in determining susceptibility to infectious diseases ofhumans. Polymorphisms in Interleukin 4 (IL-4), an anti-inflammatory cytokine thatregulates the balance between T helper (Th1) and Th2 immune responses, has beenreported to affect the risk of infectious and autoimmune diseases. In this study we aimed to investigate the possible association of variable numberof tandem repeat (VNTR) polymorphism in intron3 of IL-4 with susceptibility totyphoid fever in Sudanese patients. We analyzed the 70 bp intron-3 VNTR repeatpolymorphism in 200 DNA samples obtained from patients with blood cultureconfirmedtyphoid fever and 200 samples from randomly selected healthy controlsfrom the same city area. The intronic VNTR polymorphism was genotyped using PCR,followed by agarose gel elec trophoresis. The result showed that the genotypes of thetarget VNTR polymorphism in both cases and controls were not deviated from Hardy-Weinberg equilibrium (X 2, df=3=6.468, P˃ 0.05 and X 2, df=1=0.07, P˃ 0.5 respectively).The observed difference of the R3R3 & R2R2 genotypes among cases and controls isnot statistically significant (P= 0.09 and P=0.46 respectively). However, a significantincrease in the 2R3R genotype frequency among cases compared to the controls wasobserved (P˂0.05), indicating that the heterozygote 2R3R of VNTR polymorphism ofIL4 might affect individual susceptibility to typhoid fever in our population. https://journalskuwait.org/kjs/index.php/KJS/article/view/59Gene polymorphismKhartoumSudantyphoid feverVNTRIL-4 gene. |
| spellingShingle | Manal A. Fadl Mawada A. Aydarous Canguan Mao Afshan Yasmeen An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan Kuwait Journal of Science Gene polymorphism Khartoum Sudan typhoid fever VNTR IL-4 gene. |
| title | An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan |
| title_full | An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan |
| title_fullStr | An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan |
| title_full_unstemmed | An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan |
| title_short | An association of VNTR polymorphism in intron3 of IL-4 gene with susceptibility to typhoid fever in Khartoum State, Sudan |
| title_sort | association of vntr polymorphism in intron3 of il 4 gene with susceptibility to typhoid fever in khartoum state sudan |
| topic | Gene polymorphism Khartoum Sudan typhoid fever VNTR IL-4 gene. |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/59 |
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