Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization

Enzymes play a vital role in various industrial sectors and are essential components of many products. Hybrid enzyme-polymeric capsules were developed using polysulfone-activated carbon capsules as scaffolds. The polysulfone-activated carbon capsules with an average diameter of 2.55 mm were fabricat...

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Main Authors: Magdalena Olkiewicz, Josep M. Montornes, Ricard Garcia-Valls, Iwona Gulaczyk, Bartosz Tylkowski
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:C
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Online Access:https://www.mdpi.com/2311-5629/11/1/14
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author Magdalena Olkiewicz
Josep M. Montornes
Ricard Garcia-Valls
Iwona Gulaczyk
Bartosz Tylkowski
author_facet Magdalena Olkiewicz
Josep M. Montornes
Ricard Garcia-Valls
Iwona Gulaczyk
Bartosz Tylkowski
author_sort Magdalena Olkiewicz
collection DOAJ
description Enzymes play a vital role in various industrial sectors and are essential components of many products. Hybrid enzyme-polymeric capsules were developed using polysulfone-activated carbon capsules as scaffolds. The polysulfone-activated carbon capsules with an average diameter of 2.55 mm were fabricated by applying a phase inversion precipitation method. An increase in the amount of immobilized enzymes was observed with growth of activated carbon amount in polysulfone matrix. Enzyme immobilization was confirmed by the Bradford method, while Viscozyme<sup>®</sup> L activity in carboxymethyl cellulose hydrolysis to glucose was measured by the Reducing Sugar DNS method. The recycling of the hybrid Viscozyme<sup>®</sup> L-polysulfone/activated carbon capsules, and their reuse for subsequent cellulose hydrolysis was investigated and demonstrated repeatability of results.
format Article
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institution DOAJ
issn 2311-5629
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publishDate 2025-02-01
publisher MDPI AG
record_format Article
series C
spelling doaj-art-4afcdc0c93ff4f6b8a2d0f2d6a32da5a2025-08-20T02:42:39ZengMDPI AGC2311-56292025-02-011111410.3390/c11010014Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme ImmobilizationMagdalena Olkiewicz0Josep M. Montornes1Ricard Garcia-Valls2Iwona Gulaczyk3Bartosz Tylkowski4Eurecat, Centre Tecnològic de Catalunya, Chemical Technologies Unit, Marcel·lí Domingo 2, 43007 Tarragona, SpainEurecat, Centre Tecnològic de Catalunya, Chemical Technologies Unit, Marcel·lí Domingo 2, 43007 Tarragona, SpainEurecat, Centre Tecnològic de Catalunya, Chemical Technologies Unit, Marcel·lí Domingo 2, 43007 Tarragona, SpainFaculty of Chemistry, Adam Mickiewicz University in Poznań, ul. Uniwersytetu Poznańskiego 8, 61-614 Poznań, PolandEurecat, Centre Tecnològic de Catalunya, Chemical Technologies Unit, Marcel·lí Domingo 2, 43007 Tarragona, SpainEnzymes play a vital role in various industrial sectors and are essential components of many products. Hybrid enzyme-polymeric capsules were developed using polysulfone-activated carbon capsules as scaffolds. The polysulfone-activated carbon capsules with an average diameter of 2.55 mm were fabricated by applying a phase inversion precipitation method. An increase in the amount of immobilized enzymes was observed with growth of activated carbon amount in polysulfone matrix. Enzyme immobilization was confirmed by the Bradford method, while Viscozyme<sup>®</sup> L activity in carboxymethyl cellulose hydrolysis to glucose was measured by the Reducing Sugar DNS method. The recycling of the hybrid Viscozyme<sup>®</sup> L-polysulfone/activated carbon capsules, and their reuse for subsequent cellulose hydrolysis was investigated and demonstrated repeatability of results.https://www.mdpi.com/2311-5629/11/1/14enzyme immobilizationpolysulfone-activated carbon capsulesphase inversion precipitationpolisulfoneViscozyme<sup>®</sup> L
spellingShingle Magdalena Olkiewicz
Josep M. Montornes
Ricard Garcia-Valls
Iwona Gulaczyk
Bartosz Tylkowski
Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
C
enzyme immobilization
polysulfone-activated carbon capsules
phase inversion precipitation
polisulfone
Viscozyme<sup>®</sup> L
title Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
title_full Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
title_fullStr Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
title_full_unstemmed Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
title_short Porous Polysulfone/Activated Carbon Capsules as Scaffolds for Enzyme Immobilization
title_sort porous polysulfone activated carbon capsules as scaffolds for enzyme immobilization
topic enzyme immobilization
polysulfone-activated carbon capsules
phase inversion precipitation
polisulfone
Viscozyme<sup>®</sup> L
url https://www.mdpi.com/2311-5629/11/1/14
work_keys_str_mv AT magdalenaolkiewicz porouspolysulfoneactivatedcarboncapsulesasscaffoldsforenzymeimmobilization
AT josepmmontornes porouspolysulfoneactivatedcarboncapsulesasscaffoldsforenzymeimmobilization
AT ricardgarciavalls porouspolysulfoneactivatedcarboncapsulesasscaffoldsforenzymeimmobilization
AT iwonagulaczyk porouspolysulfoneactivatedcarboncapsulesasscaffoldsforenzymeimmobilization
AT bartosztylkowski porouspolysulfoneactivatedcarboncapsulesasscaffoldsforenzymeimmobilization