Formulation of synbiotic buttermilk powder with higher viability of probiotic cells

The present study focused on the use of trehalose (TRE), whey protein concentrate (WPC), pullulan (PUL), and calcium lactate gluconate (CAL) as a protectant in the formulation of synbiotic buttermilk powder by application of spray drying. The spray drying of the solution mixture was carried out at a...

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Main Authors: Anu Ahlawat, Harsh B. Jadhav, Laxmi Ananthanarayan
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Food Chemistry Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772753X24002223
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author Anu Ahlawat
Harsh B. Jadhav
Laxmi Ananthanarayan
author_facet Anu Ahlawat
Harsh B. Jadhav
Laxmi Ananthanarayan
author_sort Anu Ahlawat
collection DOAJ
description The present study focused on the use of trehalose (TRE), whey protein concentrate (WPC), pullulan (PUL), and calcium lactate gluconate (CAL) as a protectant in the formulation of synbiotic buttermilk powder by application of spray drying. The spray drying of the solution mixture was carried out at an inlet temperature of 150 °C, a feed flow rate of 15mL/min, and compressed air at 3 kg/cm2 while keeping the outlet temperature constant at 70 ± 5 °C. TRE4 + CAL0.5 showed good results with the viability of Bifidobacterium bifidum as 6.04 ± 0.13 after 16 weeks followed by TRE2 + CAL0.5, TRE4, CAL0.5, WPC6, PUL4, and Control. The addition of protectants and Fructooligosaccharide (FOS) as a prebiotic did not affect the powder properties. The moisture content, water activity, bulk density, hygroscopicity, solubility, particle size, and scanning electron microscopy (SEM) of SBMP were better than the control sample for 17 weeks. SEM results showed spherical shapes without any cracks for SBMP containing TRE4 + CAL0.5. The present work demonstrated that the formulation containing TRE4 + CAL0.5 showed excellent results in terms of the viability of probiotic cells and also physicochemical properties of powder during storage for 16 weeks at 4 ± 1 °C.
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spelling doaj-art-4af2ce1204704d2b810c16c32093f7922025-08-20T01:59:34ZengElsevierFood Chemistry Advances2772-753X2024-12-01510082710.1016/j.focha.2024.100827Formulation of synbiotic buttermilk powder with higher viability of probiotic cellsAnu Ahlawat0Harsh B. Jadhav1Laxmi Ananthanarayan2Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, 400019, IndiaDepartment of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, 400019, India; Amity Institute of Biotechnology, Amity University Jaipur, Rajasthan, India; Corresponding authors.Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, 400019, India; Corresponding authors.The present study focused on the use of trehalose (TRE), whey protein concentrate (WPC), pullulan (PUL), and calcium lactate gluconate (CAL) as a protectant in the formulation of synbiotic buttermilk powder by application of spray drying. The spray drying of the solution mixture was carried out at an inlet temperature of 150 °C, a feed flow rate of 15mL/min, and compressed air at 3 kg/cm2 while keeping the outlet temperature constant at 70 ± 5 °C. TRE4 + CAL0.5 showed good results with the viability of Bifidobacterium bifidum as 6.04 ± 0.13 after 16 weeks followed by TRE2 + CAL0.5, TRE4, CAL0.5, WPC6, PUL4, and Control. The addition of protectants and Fructooligosaccharide (FOS) as a prebiotic did not affect the powder properties. The moisture content, water activity, bulk density, hygroscopicity, solubility, particle size, and scanning electron microscopy (SEM) of SBMP were better than the control sample for 17 weeks. SEM results showed spherical shapes without any cracks for SBMP containing TRE4 + CAL0.5. The present work demonstrated that the formulation containing TRE4 + CAL0.5 showed excellent results in terms of the viability of probiotic cells and also physicochemical properties of powder during storage for 16 weeks at 4 ± 1 °C.http://www.sciencedirect.com/science/article/pii/S2772753X24002223ButtermilkSpray dryingTrehalosePullulanProbiotic cellsProtectants
spellingShingle Anu Ahlawat
Harsh B. Jadhav
Laxmi Ananthanarayan
Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
Food Chemistry Advances
Buttermilk
Spray drying
Trehalose
Pullulan
Probiotic cells
Protectants
title Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
title_full Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
title_fullStr Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
title_full_unstemmed Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
title_short Formulation of synbiotic buttermilk powder with higher viability of probiotic cells
title_sort formulation of synbiotic buttermilk powder with higher viability of probiotic cells
topic Buttermilk
Spray drying
Trehalose
Pullulan
Probiotic cells
Protectants
url http://www.sciencedirect.com/science/article/pii/S2772753X24002223
work_keys_str_mv AT anuahlawat formulationofsynbioticbuttermilkpowderwithhigherviabilityofprobioticcells
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AT laxmiananthanarayan formulationofsynbioticbuttermilkpowderwithhigherviabilityofprobioticcells