Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2
To examine the gene expression of ghrelin, a growth hormone releasing and appetite stimulating hormone from stomach, we constructed human ghrelin promoter-reporter vectors and analyzed the promoter activity. The ghrelin promoter activity was high when cultured cells that express ghrelin mRNA endogen...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/580908 |
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| _version_ | 1850107450059915264 |
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| author | Yuki Shiimura Hideko Ohgusu Takahiro Sato Masayasu Kojima |
| author_facet | Yuki Shiimura Hideko Ohgusu Takahiro Sato Masayasu Kojima |
| author_sort | Yuki Shiimura |
| collection | DOAJ |
| description | To examine the gene expression of ghrelin, a growth hormone releasing and appetite stimulating hormone from stomach, we constructed human ghrelin promoter-reporter vectors and analyzed the promoter activity. The ghrelin promoter activity was high when cultured cells that express ghrelin mRNA endogenously like TT or ECC10 cells were used, indicating that these cells contain factors necessary for full expression of the human ghrelin gene. The human ghrelin promoter contains both positive and negative regulatory regions. A transient decrease of the promoter activity was found when the reporter vector with the −1600 fragment of the human ghrelin promoter was transfected into cultured cells. We then examined the effect of several transcription factors on the ghrelin promoter activity and found that NF-κB suppressed and that Nkx2.2, a homeodomain-containing transcription factor that is important for ghrelin cell development in pancreas, activates the promoter activity. These transcription factors may be possible targets for the control of ghrelin gene expression. |
| format | Article |
| id | doaj-art-4af0cda12b1f4e1c92ce0c58fd45f1dc |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-4af0cda12b1f4e1c92ce0c58fd45f1dc2025-08-20T02:38:35ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/580908580908Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2Yuki Shiimura0Hideko Ohgusu1Takahiro Sato2Masayasu Kojima3Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0864, JapanMolecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0864, JapanMolecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0864, JapanMolecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0864, JapanTo examine the gene expression of ghrelin, a growth hormone releasing and appetite stimulating hormone from stomach, we constructed human ghrelin promoter-reporter vectors and analyzed the promoter activity. The ghrelin promoter activity was high when cultured cells that express ghrelin mRNA endogenously like TT or ECC10 cells were used, indicating that these cells contain factors necessary for full expression of the human ghrelin gene. The human ghrelin promoter contains both positive and negative regulatory regions. A transient decrease of the promoter activity was found when the reporter vector with the −1600 fragment of the human ghrelin promoter was transfected into cultured cells. We then examined the effect of several transcription factors on the ghrelin promoter activity and found that NF-κB suppressed and that Nkx2.2, a homeodomain-containing transcription factor that is important for ghrelin cell development in pancreas, activates the promoter activity. These transcription factors may be possible targets for the control of ghrelin gene expression.http://dx.doi.org/10.1155/2015/580908 |
| spellingShingle | Yuki Shiimura Hideko Ohgusu Takahiro Sato Masayasu Kojima Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 International Journal of Endocrinology |
| title | Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 |
| title_full | Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 |
| title_fullStr | Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 |
| title_full_unstemmed | Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 |
| title_short | Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2 |
| title_sort | regulation of the human ghrelin promoter activity by transcription factors nf κb and nkx2 2 |
| url | http://dx.doi.org/10.1155/2015/580908 |
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