Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness
Introduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy. Methodology: Two hundred and nine undiagnosed cryoprese...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2016-08-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/7573 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850147947690328064 |
|---|---|
| author | Ageng Wiyatno Ungke Antonjaya Chairin Nisa Ma'roef Silvita Fitri Riswari Hofiya Djauhari I Made Artika Corina Monagin Bradley S. Schneider Khin Saw Myint Bachti Alisjahbana Dodi Safari Herman Kosasih |
| author_facet | Ageng Wiyatno Ungke Antonjaya Chairin Nisa Ma'roef Silvita Fitri Riswari Hofiya Djauhari I Made Artika Corina Monagin Bradley S. Schneider Khin Saw Myint Bachti Alisjahbana Dodi Safari Herman Kosasih |
| author_sort | Ageng Wiyatno |
| collection | DOAJ |
| description | Introduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy.
Methodology: Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using Enterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells.
Results: CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation.
Conclusions: We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia.
|
| format | Article |
| id | doaj-art-4ae7aaeb3cd14c40a042a5b4b5f7091c |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-4ae7aaeb3cd14c40a042a5b4b5f7091c2025-08-20T02:27:23ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-08-01100810.3855/jidc.7573Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illnessAgeng Wiyatno0Ungke Antonjaya1Chairin Nisa Ma'roef2Silvita Fitri Riswari3Hofiya Djauhari4I Made Artika5Corina Monagin6Bradley S. Schneider7Khin Saw Myint8Bachti Alisjahbana9Dodi Safari10Herman Kosasih11Eijkman Institute for Molecular Biology, Jakarta, IndonesiaEijkman-Oxford Clinical Research Unit, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaUniversitas Padjadjaran, Bandung, IndonesiaUniversitas Padjadjaran, Bandung, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaMetabiota, Incorporation, San Francisco, United StatesMetabiota, Incorporation, San Francisco, United StatesEijkman Institute for Molecular Biology, Jakarta, IndonesiaUniversitas Padjadjaran, Bandung, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaUniversitas Padjadjaran, Bandung, IndonesiaIntroduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy. Methodology: Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using Enterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells. Results: CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation. Conclusions: We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia. https://jidc.org/index.php/journal/article/view/7573CoxsackievirusIndonesiafebrile illness |
| spellingShingle | Ageng Wiyatno Ungke Antonjaya Chairin Nisa Ma'roef Silvita Fitri Riswari Hofiya Djauhari I Made Artika Corina Monagin Bradley S. Schneider Khin Saw Myint Bachti Alisjahbana Dodi Safari Herman Kosasih Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness Journal of Infection in Developing Countries Coxsackievirus Indonesia febrile illness |
| title | Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness |
| title_full | Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness |
| title_fullStr | Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness |
| title_full_unstemmed | Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness |
| title_short | Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness |
| title_sort | detection and identification of coxsackievirus b3 from sera of an indonesian patient with undifferentiated febrile illness |
| topic | Coxsackievirus Indonesia febrile illness |
| url | https://jidc.org/index.php/journal/article/view/7573 |
| work_keys_str_mv | AT agengwiyatno detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT ungkeantonjaya detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT chairinnisamaroef detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT silvitafitririswari detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT hofiyadjauhari detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT imadeartika detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT corinamonagin detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT bradleysschneider detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT khinsawmyint detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT bachtialisjahbana detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT dodisafari detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness AT hermankosasih detectionandidentificationofcoxsackievirusb3fromseraofanindonesianpatientwithundifferentiatedfebrileillness |