Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities
This study aims to investigate the phytoconstituents of the chloroform fraction of three Cystoseira spp. namely C. myrica, C. trinodis, and C. tamariscifolia using UPLC/ESI/MS technique. The results revealed the identification of 19, 20 and 11 metabolites in C. myrica, C. trinodis, and C. tamariscif...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14756366.2023.2292482 |
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| author | Shaza H. Aly Ahmed M. Elissawy Mahmoud A. El Hassab Taghreed A. Majrashi Fatma E. Hassan Eslam B. Elkaeed Wagdy M. Eldehna Abdel Nasser B. Singab |
| author_facet | Shaza H. Aly Ahmed M. Elissawy Mahmoud A. El Hassab Taghreed A. Majrashi Fatma E. Hassan Eslam B. Elkaeed Wagdy M. Eldehna Abdel Nasser B. Singab |
| author_sort | Shaza H. Aly |
| collection | DOAJ |
| description | This study aims to investigate the phytoconstituents of the chloroform fraction of three Cystoseira spp. namely C. myrica, C. trinodis, and C. tamariscifolia using UPLC/ESI/MS technique. The results revealed the identification of 19, 20 and 11 metabolites in C. myrica, C. trinodis, and C. tamariscifolia, respectively mainly terpenoids, flavonoids, phenolic acids and fatty acids. Also, an in vitro antioxidant study using FRAP and DPPH assays was conducted where the chloroform fraction of C. trinodis displayed the highest antioxidant activity in both assays, which would be attributed to its highest total phenolics and total flavonoids. Besides, the investigation of COX-1, α-glucosidase and α-amylase inhibitory activities were performed. Regarding C. trinodis, it showed the strongest inhibitory activity towards COX-1. Moreover, it showed potent inhibitory activity towards α-glucosidase and α-amylase enzymes. According to the molecular docking studies, the major compounds characterised showed efficient binding to the active sites of the target enzymes. |
| format | Article |
| id | doaj-art-4ae60fd81e38457ebec5e168c4931d67 |
| institution | OA Journals |
| issn | 1475-6366 1475-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj-art-4ae60fd81e38457ebec5e168c4931d672025-08-20T01:57:45ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742024-12-0139110.1080/14756366.2023.2292482Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activitiesShaza H. Aly0Ahmed M. Elissawy1Mahmoud A. El Hassab2Taghreed A. Majrashi3Fatma E. Hassan4Eslam B. Elkaeed5Wagdy M. Eldehna6Abdel Nasser B. Singab7Department of Pharmacognosy, Badr University in Cairo (BUC), Badr City, EgyptDepartment of Pharmacognosy, Ain-Shams University, Cairo, EgyptDepartment of Medicinal Chemistry, King Salman International University (KSIU), South Sinai, EgyptDepartment of Pharmacognosy, College of Pharmacy, King Khalid University, Asir, Saudi ArabiaDepartment of Physiology, General Medicine Practice Program, Batterjee Medical College, Jeddah, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Pharmacognosy, Ain-Shams University, Cairo, EgyptThis study aims to investigate the phytoconstituents of the chloroform fraction of three Cystoseira spp. namely C. myrica, C. trinodis, and C. tamariscifolia using UPLC/ESI/MS technique. The results revealed the identification of 19, 20 and 11 metabolites in C. myrica, C. trinodis, and C. tamariscifolia, respectively mainly terpenoids, flavonoids, phenolic acids and fatty acids. Also, an in vitro antioxidant study using FRAP and DPPH assays was conducted where the chloroform fraction of C. trinodis displayed the highest antioxidant activity in both assays, which would be attributed to its highest total phenolics and total flavonoids. Besides, the investigation of COX-1, α-glucosidase and α-amylase inhibitory activities were performed. Regarding C. trinodis, it showed the strongest inhibitory activity towards COX-1. Moreover, it showed potent inhibitory activity towards α-glucosidase and α-amylase enzymes. According to the molecular docking studies, the major compounds characterised showed efficient binding to the active sites of the target enzymes.https://www.tandfonline.com/doi/10.1080/14756366.2023.2292482Antioxidantsanti-inflammatoryantihyperglycemicbrown algaemolecular docking |
| spellingShingle | Shaza H. Aly Ahmed M. Elissawy Mahmoud A. El Hassab Taghreed A. Majrashi Fatma E. Hassan Eslam B. Elkaeed Wagdy M. Eldehna Abdel Nasser B. Singab Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities Journal of Enzyme Inhibition and Medicinal Chemistry Antioxidants anti-inflammatory antihyperglycemic brown algae molecular docking |
| title | Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities |
| title_full | Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities |
| title_fullStr | Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities |
| title_full_unstemmed | Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities |
| title_short | Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities |
| title_sort | comparative metabolic study of the chloroform fraction of three cystoseira species based on uplc esi ms analysis and biological activities |
| topic | Antioxidants anti-inflammatory antihyperglycemic brown algae molecular docking |
| url | https://www.tandfonline.com/doi/10.1080/14756366.2023.2292482 |
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