MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria
Abstract Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we demonstrate that MamF-like proteins are crucial for the formation and functionality of bacterial magnetosome o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55121-0 |
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| author | Anja Paulus Frederik Ahrens Annika Schraut Hannah Hofmann Tim Schiller Thomas Sura Dörte Becher René Uebe |
| author_facet | Anja Paulus Frederik Ahrens Annika Schraut Hannah Hofmann Tim Schiller Thomas Sura Dörte Becher René Uebe |
| author_sort | Anja Paulus |
| collection | DOAJ |
| description | Abstract Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we demonstrate that MamF-like proteins are crucial for the formation and functionality of bacterial magnetosome organelles. Deletion of mamF-like genes in the Alphaproteobacterium Magnetospirillum gryphiswaldense results in severe defects in organelle positioning, biomineralization, and magnetic navigation. These phenotypic defects result from the disrupted targeting of a subset of magnetosomal proteins that contain C-terminal glycine-rich integral membrane domains. Phylogenetic analyses reveal an ancient evolutionary link between MamF-like proteins and plastidial Tic20. Our findings redefine the molecular roles of MamF-like proteins and suggest that organelle-specific protein targeting systems also play a role in bacterial organelle formation. |
| format | Article |
| id | doaj-art-4ae46ec83bf241b3b8bad17ed2e7db8d |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-4ae46ec83bf241b3b8bad17ed2e7db8d2025-08-20T02:35:51ZengNature PortfolioNature Communications2041-17232024-12-0115111610.1038/s41467-024-55121-0MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteriaAnja Paulus0Frederik Ahrens1Annika Schraut2Hannah Hofmann3Tim Schiller4Thomas Sura5Dörte Becher6René Uebe7Department of Microbiology, University of BayreuthDepartment of Microbiology, University of BayreuthDepartment of Microbiology, University of BayreuthDepartment of Microbiology, University of BayreuthDepartment of Microbiology, University of BayreuthMicrobial Proteomics, Institute of Microbiology, University of GreifswaldMicrobial Proteomics, Institute of Microbiology, University of GreifswaldDepartment of Microbiology, University of BayreuthAbstract Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we demonstrate that MamF-like proteins are crucial for the formation and functionality of bacterial magnetosome organelles. Deletion of mamF-like genes in the Alphaproteobacterium Magnetospirillum gryphiswaldense results in severe defects in organelle positioning, biomineralization, and magnetic navigation. These phenotypic defects result from the disrupted targeting of a subset of magnetosomal proteins that contain C-terminal glycine-rich integral membrane domains. Phylogenetic analyses reveal an ancient evolutionary link between MamF-like proteins and plastidial Tic20. Our findings redefine the molecular roles of MamF-like proteins and suggest that organelle-specific protein targeting systems also play a role in bacterial organelle formation.https://doi.org/10.1038/s41467-024-55121-0 |
| spellingShingle | Anja Paulus Frederik Ahrens Annika Schraut Hannah Hofmann Tim Schiller Thomas Sura Dörte Becher René Uebe MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria Nature Communications |
| title | MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria |
| title_full | MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria |
| title_fullStr | MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria |
| title_full_unstemmed | MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria |
| title_short | MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria |
| title_sort | mamf like proteins are distant tic20 homologs involved in organelle assembly in bacteria |
| url | https://doi.org/10.1038/s41467-024-55121-0 |
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