Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats
This study aimed to determine whether C. mannii seed oil (CMSO) could shield rats from cyclophosphamide-induced hepatorenal toxicity. The focus was on its antioxidant and anti-inflammatory properties. We randomly assigned thirty-six (36) male Wistar rats into six groups of six rats each. Groups A an...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025000039 |
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| author | Amaka Cecilia Agbara Ejike Daniel Eze Christian Emeka Offor Adam Moyosore Afodun Ezebuilo Ugbala Ekpono Peter Chinedu Agu Chinyere Aloke Nkeiru Nwaamaka Ezeani Emmanuel Orire Ikuomola Ekom Monday Etukudo Ilemobayo Victor Fasogbon Angela Mumbua Musyoka Patrick Maduabuchi Aja |
| author_facet | Amaka Cecilia Agbara Ejike Daniel Eze Christian Emeka Offor Adam Moyosore Afodun Ezebuilo Ugbala Ekpono Peter Chinedu Agu Chinyere Aloke Nkeiru Nwaamaka Ezeani Emmanuel Orire Ikuomola Ekom Monday Etukudo Ilemobayo Victor Fasogbon Angela Mumbua Musyoka Patrick Maduabuchi Aja |
| author_sort | Amaka Cecilia Agbara |
| collection | DOAJ |
| description | This study aimed to determine whether C. mannii seed oil (CMSO) could shield rats from cyclophosphamide-induced hepatorenal toxicity. The focus was on its antioxidant and anti-inflammatory properties. We randomly assigned thirty-six (36) male Wistar rats into six groups of six rats each. Groups A and B served as normal and negative controls, respectively. Group C, the standard control, was administered 300 mg/kg body weight (bw) of Omega 3 oil for 27 days, followed by 100 mg/kg bw of cyclophosphamide on day 28. Group D, E, and F received 5, 2.5, and 1.5 ml/kg b.w. of CMSO for 27 days, then 100 ml/kg b.w. of cyclophosphamide on day 28. We measured the body weights of the experimental rats every week. Rats of all groups were sacrificed on day 30 and collected blood for biochemical analysis using standard methods. The phytochemical constituents were determined by the spectrophotometric method. The phytochemical study of CMSO indicated the relative composition of constituents(mg/100g) as phenols (30 %), tannins (20 %), flavonoids (18 %), terpenoids (15 %), glycosides (10 %), alkaloids (5 %), and HCN (2 %) in Cucumeropsis mannii seed oil. When cyclophosphamide was given to Wistar albino rats, it greatly decreased the activities of catalase (CAT) and superoxide dismutase (SOD), but it increased the activities of iNOS and the levels of MDA and IL-1β. Cyclophosphamide increased ALT (52.3 U/L), AST (48.7 U/L), ALP (46.5 U/L), total bilirubin (1.4 mg/dL), conjugated bilirubin (0.8 mg/dL), creatinine (0.8 mg/dL), urea (45.6 mg/dL), and BUN (15.7 mg/dL), with a reduction in the albumin (4.8 g/dL) level. Cyclophosphamide administration also caused hepatocellular necrosis, inflammatory leukocyte infiltration, tubular necrosis, and proteinaceous deposits in Bowman's space in the kidney. However, combining CMSO and Omega 3 oils significantly restored the altered histology architecture, antioxidant, inflammatory markers, and liver and kidney function parameters to levels comparable to the normal group, thereby reducing the harmful effects of cyclophosphamide. This effect did not depend on the dose. These results suggest that CMSO, being an antioxidant and an anti-inflammatory, could potentially aid in preventing and treating hepatorenal toxicity resulting from cyclophosphamide. |
| format | Article |
| id | doaj-art-4ae42c82d0364bb39391fbdea1548637 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-4ae42c82d0364bb39391fbdea15486372025-02-02T05:27:54ZengElsevierHeliyon2405-84402025-01-01112e41624Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in ratsAmaka Cecilia Agbara0Ejike Daniel Eze1Christian Emeka Offor2Adam Moyosore Afodun3Ezebuilo Ugbala Ekpono4Peter Chinedu Agu5Chinyere Aloke6Nkeiru Nwaamaka Ezeani7Emmanuel Orire Ikuomola8Ekom Monday Etukudo9Ilemobayo Victor Fasogbon10Angela Mumbua Musyoka11Patrick Maduabuchi Aja12Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, NigeriaDepartment of Physiology, School of Medicine, Kabale University, Kabale, UgandaDepartment of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, NigeriaDepartment of Anatomy and Cell Biology, Faculty of Health Sciences, Busitema University, UgandaDepartment of Science Laboratory Technology, Biochemistry Option, Federal Polytechnic Oko, Anambra State, NigeriaDepartment of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, NigeriaDepartment of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria; Protein Structure-Function and Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Braamfontein, 2050, Johannesburg, South AfricaDepartment of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, NigeriaDepartment of Physiology, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, UgandaDepartment of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria; Department of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Uganda; Corresponding author. Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.This study aimed to determine whether C. mannii seed oil (CMSO) could shield rats from cyclophosphamide-induced hepatorenal toxicity. The focus was on its antioxidant and anti-inflammatory properties. We randomly assigned thirty-six (36) male Wistar rats into six groups of six rats each. Groups A and B served as normal and negative controls, respectively. Group C, the standard control, was administered 300 mg/kg body weight (bw) of Omega 3 oil for 27 days, followed by 100 mg/kg bw of cyclophosphamide on day 28. Group D, E, and F received 5, 2.5, and 1.5 ml/kg b.w. of CMSO for 27 days, then 100 ml/kg b.w. of cyclophosphamide on day 28. We measured the body weights of the experimental rats every week. Rats of all groups were sacrificed on day 30 and collected blood for biochemical analysis using standard methods. The phytochemical constituents were determined by the spectrophotometric method. The phytochemical study of CMSO indicated the relative composition of constituents(mg/100g) as phenols (30 %), tannins (20 %), flavonoids (18 %), terpenoids (15 %), glycosides (10 %), alkaloids (5 %), and HCN (2 %) in Cucumeropsis mannii seed oil. When cyclophosphamide was given to Wistar albino rats, it greatly decreased the activities of catalase (CAT) and superoxide dismutase (SOD), but it increased the activities of iNOS and the levels of MDA and IL-1β. Cyclophosphamide increased ALT (52.3 U/L), AST (48.7 U/L), ALP (46.5 U/L), total bilirubin (1.4 mg/dL), conjugated bilirubin (0.8 mg/dL), creatinine (0.8 mg/dL), urea (45.6 mg/dL), and BUN (15.7 mg/dL), with a reduction in the albumin (4.8 g/dL) level. Cyclophosphamide administration also caused hepatocellular necrosis, inflammatory leukocyte infiltration, tubular necrosis, and proteinaceous deposits in Bowman's space in the kidney. However, combining CMSO and Omega 3 oils significantly restored the altered histology architecture, antioxidant, inflammatory markers, and liver and kidney function parameters to levels comparable to the normal group, thereby reducing the harmful effects of cyclophosphamide. This effect did not depend on the dose. These results suggest that CMSO, being an antioxidant and an anti-inflammatory, could potentially aid in preventing and treating hepatorenal toxicity resulting from cyclophosphamide.http://www.sciencedirect.com/science/article/pii/S2405844025000039Prophylactic efficacyC. mannii seed oilcyclophosphamideLiver and kidney toxicity |
| spellingShingle | Amaka Cecilia Agbara Ejike Daniel Eze Christian Emeka Offor Adam Moyosore Afodun Ezebuilo Ugbala Ekpono Peter Chinedu Agu Chinyere Aloke Nkeiru Nwaamaka Ezeani Emmanuel Orire Ikuomola Ekom Monday Etukudo Ilemobayo Victor Fasogbon Angela Mumbua Musyoka Patrick Maduabuchi Aja Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats Heliyon Prophylactic efficacy C. mannii seed oil cyclophosphamide Liver and kidney toxicity |
| title | Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats |
| title_full | Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats |
| title_fullStr | Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats |
| title_full_unstemmed | Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats |
| title_short | Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats |
| title_sort | cucumeropsis mannii seed oil shields against cyclophosphamide induced hepatorenal toxicity by modulating redox imbalance and inos il β1 signalling in rats |
| topic | Prophylactic efficacy C. mannii seed oil cyclophosphamide Liver and kidney toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025000039 |
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