The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing
Manual processing of lipoaspirate can enhance stem cell concentration, thereby improving the take rate, which still represents a major challenge in autologous fat transfer. However, since the preparation consists of many manual steps that are difficult to standardize, we investigated the influence o...
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2025-04-01
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| author | Andreas Eigenberger Oliver Felthaus Alexander Bartsch Tom Schimanski Kirsten Utpatel Lukas Prantl |
| author_facet | Andreas Eigenberger Oliver Felthaus Alexander Bartsch Tom Schimanski Kirsten Utpatel Lukas Prantl |
| author_sort | Andreas Eigenberger |
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| description | Manual processing of lipoaspirate can enhance stem cell concentration, thereby improving the take rate, which still represents a major challenge in autologous fat transfer. However, since the preparation consists of many manual steps that are difficult to standardize, we investigated the influence of residual tumescent solution on the macroscopic and microscopic outcome of the mechanically processed lipoaspirate. Additionally, we investigated whether sedimentation followed by vacuum filtration of the aqueous phase could accelerate processing by replacing the initial centrifugation step. Samples with more than 5% remaining aqueous phase show no clearly defined oil phase, preventing any volume reduction. In contrast, all centrifuged samples produced a clear oil phase. The remaining tissue, as confirmed by both histology and viability assays, was superior to nanofat. Although sedimentation and filtration in the LipoCollector did not sufficiently separate enough aqueous phase from the lipoaspirate, tissue viability was significantly higher compared to our control container. Our findings indicate that centrifugation remains essential for effective aqueous phase separation and further mechanical processing, while the automatic filtration may enhance processing efficiency. These results indicate that further work is needed to simplify mechanical processing, as the outcome can be significantly influenced by parameters such as tumescent impurities. |
| format | Article |
| id | doaj-art-4addc1aa4daf45c29cf75aa084becd46 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-4addc1aa4daf45c29cf75aa084becd462025-08-20T03:14:20ZengMDPI AGCells2073-44092025-04-0114860110.3390/cells14080601The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical ProcessingAndreas Eigenberger0Oliver Felthaus1Alexander Bartsch2Tom Schimanski3Kirsten Utpatel4Lukas Prantl5Department of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyMedical Device Lab, Regensburg Center of Biomedical Engineering (RCBE), Faculty of Mechanical Engineering, Ostbayerische Technische Hochschule Regensburg, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyInstitute of Pathology, University of Regensburg, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyManual processing of lipoaspirate can enhance stem cell concentration, thereby improving the take rate, which still represents a major challenge in autologous fat transfer. However, since the preparation consists of many manual steps that are difficult to standardize, we investigated the influence of residual tumescent solution on the macroscopic and microscopic outcome of the mechanically processed lipoaspirate. Additionally, we investigated whether sedimentation followed by vacuum filtration of the aqueous phase could accelerate processing by replacing the initial centrifugation step. Samples with more than 5% remaining aqueous phase show no clearly defined oil phase, preventing any volume reduction. In contrast, all centrifuged samples produced a clear oil phase. The remaining tissue, as confirmed by both histology and viability assays, was superior to nanofat. Although sedimentation and filtration in the LipoCollector did not sufficiently separate enough aqueous phase from the lipoaspirate, tissue viability was significantly higher compared to our control container. Our findings indicate that centrifugation remains essential for effective aqueous phase separation and further mechanical processing, while the automatic filtration may enhance processing efficiency. These results indicate that further work is needed to simplify mechanical processing, as the outcome can be significantly influenced by parameters such as tumescent impurities.https://www.mdpi.com/2073-4409/14/8/601autologous fat transferlipofillingintersyringe processingmechanical processingfat graftcell-enriched lipotransfer |
| spellingShingle | Andreas Eigenberger Oliver Felthaus Alexander Bartsch Tom Schimanski Kirsten Utpatel Lukas Prantl The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing Cells autologous fat transfer lipofilling intersyringe processing mechanical processing fat graft cell-enriched lipotransfer |
| title | The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing |
| title_full | The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing |
| title_fullStr | The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing |
| title_full_unstemmed | The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing |
| title_short | The Influence of Sedimentation on the Composition of the Lipoaspirate and the Effects on Further Mechanical Processing |
| title_sort | influence of sedimentation on the composition of the lipoaspirate and the effects on further mechanical processing |
| topic | autologous fat transfer lipofilling intersyringe processing mechanical processing fat graft cell-enriched lipotransfer |
| url | https://www.mdpi.com/2073-4409/14/8/601 |
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