A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees
Abstract Exclusion is a keystone of integrated pest management to prevent the introduction of pathogens. U.S. plant quarantine programs employ PCR and high-throughput sequencing (HTS) to test imported plants for viruses and viroids of concern. Achieving a low limit of detection in any HTS protocol c...
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Main Authors: | Francisco Mosquera-Yuqui, Daniel Ramos-Lopez, Xiaojun Hu, Yu Yang, Joshua L. Mendoza, Emmanuel Asare, Joshua Habiger, Oscar P. Hurtado-Gonzales, Andres S. Espindola |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2025-01-01
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Series: | Scientific Reports |
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-025-86065-0 |
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