A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees

A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees

Abstract Exclusion is a keystone of integrated pest management to prevent the introduction of pathogens. U.S. plant quarantine programs employ PCR and high-throughput sequencing (HTS) to test imported plants for viruses and viroids of concern. Achieving a low limit of detection in any HTS protocol c...

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Bibliographic Details
Main Authors: Francisco Mosquera-Yuqui, Daniel Ramos-Lopez, Xiaojun Hu, Yu Yang, Joshua L. Mendoza, Emmanuel Asare, Joshua Habiger, Oscar P. Hurtado-Gonzales, Andres S. Espindola
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
High throughput sequencing
Nanopore
Illumina
RNA viruses
Online Access:https://doi.org/10.1038/s41598-025-86065-0
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