Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation
ABSTRACT B cell maturation is crucial for effective adaptive immunity. It requires a complex signalling network to mediate antibody diversification through mutagenesis. B cells also rely on queues from other cells within the germinal centre. Recently, a novel class of intercellular signals mediated...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Extracellular Vesicles |
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| Online Access: | https://doi.org/10.1002/jev2.70040 |
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| author | Kevin Ho Wai Yim Ala'a Al Hrout Richard Chahwan |
| author_facet | Kevin Ho Wai Yim Ala'a Al Hrout Richard Chahwan |
| author_sort | Kevin Ho Wai Yim |
| collection | DOAJ |
| description | ABSTRACT B cell maturation is crucial for effective adaptive immunity. It requires a complex signalling network to mediate antibody diversification through mutagenesis. B cells also rely on queues from other cells within the germinal centre. Recently, a novel class of intercellular signals mediated by extracellular vesicles (EVs) has emerged. Studies have shown that B cell EV‐mediated signalling is involved in immune response regulation and tumorigenesis. However, the mechanistic role of B cell EVs is not yet established. We herein study the biological properties and physiological function of B cell EVs during B cell maturation. We use emerging technologies to profile B cell EV surface marker signatures at the single particle level, molecular cargo and physiological roles in B cell maturation. EV ncRNA cargo, characterised by RNA‐seq, identified an EV‐mediated novel non‐coding RNA (ncRNA) regulatory network for B cell maturation. We show that a previously uncharacterised micro‐RNA (miR‐5099) in combination with a set of long ncRNA are carried within B cell EVs and could contribute to antibody diversification. The physiological role of EVs in B cell maturation is investigated using EV blockade assays and complementation studies using diverse EV sources further confirmed the physiological role and mode of action of EVs in B cell maturation. |
| format | Article |
| id | doaj-art-4ad717de87a14dd59bb15450a0bdec31 |
| institution | DOAJ |
| issn | 2001-3078 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj-art-4ad717de87a14dd59bb15450a0bdec312025-08-20T02:41:52ZengWileyJournal of Extracellular Vesicles2001-30782025-01-01141n/an/a10.1002/jev2.70040Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell MaturationKevin Ho Wai Yim0Ala'a Al Hrout1Richard Chahwan2Institute of Experimental Immunology University of Zurich Zurich SwitzerlandInstitute of Experimental Immunology University of Zurich Zurich SwitzerlandInstitute of Experimental Immunology University of Zurich Zurich SwitzerlandABSTRACT B cell maturation is crucial for effective adaptive immunity. It requires a complex signalling network to mediate antibody diversification through mutagenesis. B cells also rely on queues from other cells within the germinal centre. Recently, a novel class of intercellular signals mediated by extracellular vesicles (EVs) has emerged. Studies have shown that B cell EV‐mediated signalling is involved in immune response regulation and tumorigenesis. However, the mechanistic role of B cell EVs is not yet established. We herein study the biological properties and physiological function of B cell EVs during B cell maturation. We use emerging technologies to profile B cell EV surface marker signatures at the single particle level, molecular cargo and physiological roles in B cell maturation. EV ncRNA cargo, characterised by RNA‐seq, identified an EV‐mediated novel non‐coding RNA (ncRNA) regulatory network for B cell maturation. We show that a previously uncharacterised micro‐RNA (miR‐5099) in combination with a set of long ncRNA are carried within B cell EVs and could contribute to antibody diversification. The physiological role of EVs in B cell maturation is investigated using EV blockade assays and complementation studies using diverse EV sources further confirmed the physiological role and mode of action of EVs in B cell maturation.https://doi.org/10.1002/jev2.70040antibody maturationextracellular vesiclesintercellular epigenomic signallingnon‐coding RNAsingle particle analysis |
| spellingShingle | Kevin Ho Wai Yim Ala'a Al Hrout Richard Chahwan Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation Journal of Extracellular Vesicles antibody maturation extracellular vesicles intercellular epigenomic signalling non‐coding RNA single particle analysis |
| title | Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation |
| title_full | Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation |
| title_fullStr | Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation |
| title_full_unstemmed | Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation |
| title_short | Intercellular Epigenomic Signalling via Extracellular Vesicles During B Cell Maturation |
| title_sort | intercellular epigenomic signalling via extracellular vesicles during b cell maturation |
| topic | antibody maturation extracellular vesicles intercellular epigenomic signalling non‐coding RNA single particle analysis |
| url | https://doi.org/10.1002/jev2.70040 |
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