Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC
Fosravuconazole, a newly developed oral antifungal medication by Eisai, has been utilized since 2018 for the treatment of tinea unguium. Its notable characteristics include high oral absorption and systemic bioavailability, which allow for a brief treatment duration of 3 months. Its mild inhibition...
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Elsevier
2025-03-01
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| Series: | Green Analytical Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772577425000126 |
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| author | Jeswin Maliyakal Mital Patel |
| author_facet | Jeswin Maliyakal Mital Patel |
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| collection | DOAJ |
| description | Fosravuconazole, a newly developed oral antifungal medication by Eisai, has been utilized since 2018 for the treatment of tinea unguium. Its notable characteristics include high oral absorption and systemic bioavailability, which allow for a brief treatment duration of 3 months. Its mild inhibition of the cytochrome P450 enzyme, which plays a role in adverse effects during polypharmacy, allows for the safe use of fosravuconazole alongside other medications, making it suitable for elderly patients. This study addresses the need for a precise quantitative method to determine “Fosravuconazole” using UV spectrophotometry and High-Performance Liquid Chromatography (HPLC). The HPLC method used an isocratic approach with a reversed-phase CHROMASIL C18 column (4.6 mm × 250 mm, 5 µm), a flow rate of 0.9 mL/min and detection at a wavelength of 287 nm. The mobile phase was a mixture of Acetonitrile and a 10 mM Ammonium Acetate buffer at pH 4.5, with the pH adjusted using acetic acid. Both methods were rigorously validated according to ICH Q2(R1) guidelines, demonstrating their suitability for the assessment of individual substances in various mixtures. To evaluate their environmental impact, AGREE, GAPI, and BAGI were used to evaluate the methods highlighting their sustainability in terms of solvent consumption, chemical and energy use, waste generation. BAGI showed that both methods possess scores above the recommended threshold score of 60, qualifying them for industrial applications. The UV method demonstrated a greener profile compared to the HPLC method. |
| format | Article |
| id | doaj-art-4ad6e4d421ad41939728f0a7ba052e7e |
| institution | Kabale University |
| issn | 2772-5774 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Green Analytical Chemistry |
| spelling | doaj-art-4ad6e4d421ad41939728f0a7ba052e7e2025-02-05T04:32:51ZengElsevierGreen Analytical Chemistry2772-57742025-03-0112100215Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLCJeswin Maliyakal0Mital Patel1Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKMs NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai, Maharashtra 400056, IndiaCorresponding author.; Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKMs NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai, Maharashtra 400056, IndiaFosravuconazole, a newly developed oral antifungal medication by Eisai, has been utilized since 2018 for the treatment of tinea unguium. Its notable characteristics include high oral absorption and systemic bioavailability, which allow for a brief treatment duration of 3 months. Its mild inhibition of the cytochrome P450 enzyme, which plays a role in adverse effects during polypharmacy, allows for the safe use of fosravuconazole alongside other medications, making it suitable for elderly patients. This study addresses the need for a precise quantitative method to determine “Fosravuconazole” using UV spectrophotometry and High-Performance Liquid Chromatography (HPLC). The HPLC method used an isocratic approach with a reversed-phase CHROMASIL C18 column (4.6 mm × 250 mm, 5 µm), a flow rate of 0.9 mL/min and detection at a wavelength of 287 nm. The mobile phase was a mixture of Acetonitrile and a 10 mM Ammonium Acetate buffer at pH 4.5, with the pH adjusted using acetic acid. Both methods were rigorously validated according to ICH Q2(R1) guidelines, demonstrating their suitability for the assessment of individual substances in various mixtures. To evaluate their environmental impact, AGREE, GAPI, and BAGI were used to evaluate the methods highlighting their sustainability in terms of solvent consumption, chemical and energy use, waste generation. BAGI showed that both methods possess scores above the recommended threshold score of 60, qualifying them for industrial applications. The UV method demonstrated a greener profile compared to the HPLC method.http://www.sciencedirect.com/science/article/pii/S2772577425000126SustainabilityBluenessFosravuconazoleAGREEComplex GAPIGreen chemistry |
| spellingShingle | Jeswin Maliyakal Mital Patel Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC Green Analytical Chemistry Sustainability Blueness Fosravuconazole AGREE Complex GAPI Green chemistry |
| title | Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC |
| title_full | Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC |
| title_fullStr | Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC |
| title_full_unstemmed | Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC |
| title_short | Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC |
| title_sort | green chemistry approaches in the analytical validation of fosravuconazole using uv spectrophotometry and hplc |
| topic | Sustainability Blueness Fosravuconazole AGREE Complex GAPI Green chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2772577425000126 |
| work_keys_str_mv | AT jeswinmaliyakal greenchemistryapproachesintheanalyticalvalidationoffosravuconazoleusinguvspectrophotometryandhplc AT mitalpatel greenchemistryapproachesintheanalyticalvalidationoffosravuconazoleusinguvspectrophotometryandhplc |