Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells
Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that a...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2018/1754085 |
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| author | Alexander Glassmann Carmen Carrillo Garcia Viktor Janzen Dominik Kraus Nadine Veit Jochen Winter Rainer Probstmeier |
| author_facet | Alexander Glassmann Carmen Carrillo Garcia Viktor Janzen Dominik Kraus Nadine Veit Jochen Winter Rainer Probstmeier |
| author_sort | Alexander Glassmann |
| collection | DOAJ |
| description | Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis. |
| format | Article |
| id | doaj-art-4ad512c3ed5440cbaa626bc996b2d9c1 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-4ad512c3ed5440cbaa626bc996b2d9c12025-08-20T03:36:26ZengWileyAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/17540851754085Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 CellsAlexander Glassmann0Carmen Carrillo Garcia1Viktor Janzen2Dominik Kraus3Nadine Veit4Jochen Winter5Rainer Probstmeier6Life Science Inkubator, Bonn, GermanyDepartment of Internal Medicine III, Division of Hematology and Oncology, University of Bonn, Bonn, GermanyDepartment of Internal Medicine III, Division of Hematology and Oncology, University of Bonn, Bonn, GermanyDepartment of Prosthodontics, Preclinical Education, and Material Science, University of Bonn, Bonn, GermanyNeuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University of Bonn, Bonn, GermanyOral Cell Biology Group, Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, GermanyNeuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University of Bonn, Bonn, GermanyCultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis.http://dx.doi.org/10.1155/2018/1754085 |
| spellingShingle | Alexander Glassmann Carmen Carrillo Garcia Viktor Janzen Dominik Kraus Nadine Veit Jochen Winter Rainer Probstmeier Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells Analytical Cellular Pathology |
| title | Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells |
| title_full | Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells |
| title_fullStr | Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells |
| title_full_unstemmed | Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells |
| title_short | Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells |
| title_sort | staurosporine induces the generation of polyploid giant cancer cells in non small cell lung carcinoma a549 cells |
| url | http://dx.doi.org/10.1155/2018/1754085 |
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