The association of HIF1A gene polymorphism and its expression with ischemic stroke
Abstract Hypoxia-inducible factor 1 A (HIF1A) is considered as a potential marker gene for ischemic stroke (IS), its gene polymorphism may affect IS risk and may be related to the IS pathological process. In this study, a total of 159 IS patients and 141 healthy controls were enrolled by case-contro...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-99418-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850172973276725248 |
|---|---|
| author | Fengjiao Yang Yun Gu Ya Yan Pengyu Wang Min Wang Jianjie Chen Xiaoshan Du Guangming Wang |
| author_facet | Fengjiao Yang Yun Gu Ya Yan Pengyu Wang Min Wang Jianjie Chen Xiaoshan Du Guangming Wang |
| author_sort | Fengjiao Yang |
| collection | DOAJ |
| description | Abstract Hypoxia-inducible factor 1 A (HIF1A) is considered as a potential marker gene for ischemic stroke (IS), its gene polymorphism may affect IS risk and may be related to the IS pathological process. In this study, a total of 159 IS patients and 141 healthy controls were enrolled by case-control study method. HIF1A three single nucleotide polymorphisms (SNPs: rs10873142, rs11549465, rs11549467) were genotyped by SNaPshot method and HIF1A protein levels was detected by enzyme-linked immunosorbent assay (ELISA), then the correlation between HIF-1 A SNPs and IS was analyzed by statistical analysis method. The genotype of HIF1A SNPs can affect the expression levels of clinical and laboratory parameters in IS patients, such as high-density lipoproteincholesterol (HDL-C), apolipoprotein A1, reactive oxygen species (ROS). HIF1A TCG haplotype is a protective factor for IS, HIF1A CCG haplotype is a risk factor for IS. However, the association between HIF1A rs10873142, rs11549465, rs11549467 genotypes and the IS risk was not statistically significant. ELISA analysis showed that the HIF1A expression of IS patients is higher than the healthy controls. Therefore, the gene polymorphism and expression of HIF1A may be related to IS pathological process, but the relationship between HIF1A gene polymorphism and the IS risk still needs further study. |
| format | Article |
| id | doaj-art-4ad20f60f0574e2dbf39d8d287254304 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4ad20f60f0574e2dbf39d8d2872543042025-08-20T02:19:57ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-99418-6The association of HIF1A gene polymorphism and its expression with ischemic strokeFengjiao Yang0Yun Gu1Ya Yan2Pengyu Wang3Min Wang4Jianjie Chen5Xiaoshan Du6Guangming Wang7School of Clinical Medicine, Dali UniversityDepartment of Pharmacy, The First Affiliated Hospital of Dali UniversityCollege of Pharmacy, Dali UniversitySchool of Clinical Medicine, Dali UniversitySchool of Clinical Medicine, Dali UniversitySchool of Clinical Medicine, Dali UniversityDepartment of Geriatrics, South District of Hefei First People’s HospitalSchool of Clinical Medicine, Dali UniversityAbstract Hypoxia-inducible factor 1 A (HIF1A) is considered as a potential marker gene for ischemic stroke (IS), its gene polymorphism may affect IS risk and may be related to the IS pathological process. In this study, a total of 159 IS patients and 141 healthy controls were enrolled by case-control study method. HIF1A three single nucleotide polymorphisms (SNPs: rs10873142, rs11549465, rs11549467) were genotyped by SNaPshot method and HIF1A protein levels was detected by enzyme-linked immunosorbent assay (ELISA), then the correlation between HIF-1 A SNPs and IS was analyzed by statistical analysis method. The genotype of HIF1A SNPs can affect the expression levels of clinical and laboratory parameters in IS patients, such as high-density lipoproteincholesterol (HDL-C), apolipoprotein A1, reactive oxygen species (ROS). HIF1A TCG haplotype is a protective factor for IS, HIF1A CCG haplotype is a risk factor for IS. However, the association between HIF1A rs10873142, rs11549465, rs11549467 genotypes and the IS risk was not statistically significant. ELISA analysis showed that the HIF1A expression of IS patients is higher than the healthy controls. Therefore, the gene polymorphism and expression of HIF1A may be related to IS pathological process, but the relationship between HIF1A gene polymorphism and the IS risk still needs further study.https://doi.org/10.1038/s41598-025-99418-6HIF1AIschemic strokeSNPsGene polymorphism |
| spellingShingle | Fengjiao Yang Yun Gu Ya Yan Pengyu Wang Min Wang Jianjie Chen Xiaoshan Du Guangming Wang The association of HIF1A gene polymorphism and its expression with ischemic stroke Scientific Reports HIF1A Ischemic stroke SNPs Gene polymorphism |
| title | The association of HIF1A gene polymorphism and its expression with ischemic stroke |
| title_full | The association of HIF1A gene polymorphism and its expression with ischemic stroke |
| title_fullStr | The association of HIF1A gene polymorphism and its expression with ischemic stroke |
| title_full_unstemmed | The association of HIF1A gene polymorphism and its expression with ischemic stroke |
| title_short | The association of HIF1A gene polymorphism and its expression with ischemic stroke |
| title_sort | association of hif1a gene polymorphism and its expression with ischemic stroke |
| topic | HIF1A Ischemic stroke SNPs Gene polymorphism |
| url | https://doi.org/10.1038/s41598-025-99418-6 |
| work_keys_str_mv | AT fengjiaoyang theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT yungu theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT yayan theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT pengyuwang theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT minwang theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT jianjiechen theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT xiaoshandu theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT guangmingwang theassociationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT fengjiaoyang associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT yungu associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT yayan associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT pengyuwang associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT minwang associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT jianjiechen associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT xiaoshandu associationofhif1agenepolymorphismanditsexpressionwithischemicstroke AT guangmingwang associationofhif1agenepolymorphismanditsexpressionwithischemicstroke |