Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor
Background: PGF2α hormone administration can improve semen quality through increased contractility of smooth muscle cells in testicular tissue and increased testosterone hormone. Immunohistochemically, the increase in contractility of smooth muscle cells and testosterone hormone can be measured by...
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| Format: | Article |
| Language: | English |
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Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=223112 |
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| author | Teuku Armansyah Husnurrizal Husnurrizal Sri Wahyuni Hafizuddin Hafizuddin Tongku Nizwan Siregar Amalia Sutriana Arman Sayuti Adinda Tri Syahrani Muhammad Bintang Pariansyah |
| author_facet | Teuku Armansyah Husnurrizal Husnurrizal Sri Wahyuni Hafizuddin Hafizuddin Tongku Nizwan Siregar Amalia Sutriana Arman Sayuti Adinda Tri Syahrani Muhammad Bintang Pariansyah |
| author_sort | Teuku Armansyah |
| collection | DOAJ |
| description | Background:
PGF2α hormone administration can improve semen quality through increased contractility of smooth muscle cells in testicular tissue and increased testosterone hormone. Immunohistochemically, the increase in contractility of smooth muscle cells and testosterone hormone can be measured by an increase in the expression of alpha-smooth muscle actin (α-SMA) and androgen receptor (AR).
Aim:
This study aims to determine the distribution and increased α-SMA expression and AR in the testis of Wistar rats after administration of PGF2α which detected using the immunohistochemistry (IHC) method.
Methods:
A total of 15 male Wistar rats (Rattus norvegicus) with body weight 200-250 g and 8-10 weeks old were used in this study. All rats were acclimated for two weeks. The rats were divided into 5 treatment groups (n=3). The rat in the control group (P0), testicular collection was carried out 30 minutes after injection of 0.5 ml NaCl. In group P1, P2, P3, and P4, the rats were intraperitoneal injected with 2.5 mg/kg BW of PGF2α (Lutalyze, Zoetis, USA), and the testis collection was performed 30 minutes, 60 minutes , 90 minutes , and 120 minutes after PGF2α injection, respectively. were processed into histology preparations and stained with immunohistochemistry staining using avidin-biotin complex peroxidase method (ABC method). The distribution of α-SMA and AR was analyzed descriptively, while the score difference of α-SMA and AR expression was analyzed using the Kruskal-Wallis test and the Mann-Whitney U Test.
Results:
The results showed that α-SMA was positively detected in peritubular myoid cells on the basal membrane of seminiferous tubules, blood vessel walls, and connective tissue. Statistical analysis showed that α-SMA expression in peritubular myoid cells and testicular connective tissue in P2 (60-minute interval) was significantly different than other treatment groups (P0, P1, P3, and P4) (P0.05). AR was positively detected in connective tissue, peritubular myoid cells, Leydig cells, Sertoli cells, and blood vessels. Statistical analysis showed significant differences in AR expression in peritubular myoid cells and Sertoli cells (P [Open Vet J 2024; 14(12.000): 3474-3486] |
| format | Article |
| id | doaj-art-4ad0c6e8679d48629538a3b4a8e7b1ea |
| institution | DOAJ |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-4ad0c6e8679d48629538a3b4a8e7b1ea2025-08-20T03:09:13ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123474348610.5455/OVJ.2024.v14.i12.31223112Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptorTeuku Armansyah0Husnurrizal Husnurrizal1Sri Wahyuni2Hafizuddin Hafizuddin3Tongku Nizwan Siregar4Amalia Sutriana5Arman SayutiAdinda Tri SyahraniMuhammad Bintang PariansyahDarussalam Darussalam Darussalam Darussalam Darussalam DarussalamBackground: PGF2α hormone administration can improve semen quality through increased contractility of smooth muscle cells in testicular tissue and increased testosterone hormone. Immunohistochemically, the increase in contractility of smooth muscle cells and testosterone hormone can be measured by an increase in the expression of alpha-smooth muscle actin (α-SMA) and androgen receptor (AR). Aim: This study aims to determine the distribution and increased α-SMA expression and AR in the testis of Wistar rats after administration of PGF2α which detected using the immunohistochemistry (IHC) method. Methods: A total of 15 male Wistar rats (Rattus norvegicus) with body weight 200-250 g and 8-10 weeks old were used in this study. All rats were acclimated for two weeks. The rats were divided into 5 treatment groups (n=3). The rat in the control group (P0), testicular collection was carried out 30 minutes after injection of 0.5 ml NaCl. In group P1, P2, P3, and P4, the rats were intraperitoneal injected with 2.5 mg/kg BW of PGF2α (Lutalyze, Zoetis, USA), and the testis collection was performed 30 minutes, 60 minutes , 90 minutes , and 120 minutes after PGF2α injection, respectively. were processed into histology preparations and stained with immunohistochemistry staining using avidin-biotin complex peroxidase method (ABC method). The distribution of α-SMA and AR was analyzed descriptively, while the score difference of α-SMA and AR expression was analyzed using the Kruskal-Wallis test and the Mann-Whitney U Test. Results: The results showed that α-SMA was positively detected in peritubular myoid cells on the basal membrane of seminiferous tubules, blood vessel walls, and connective tissue. Statistical analysis showed that α-SMA expression in peritubular myoid cells and testicular connective tissue in P2 (60-minute interval) was significantly different than other treatment groups (P0, P1, P3, and P4) (P0.05). AR was positively detected in connective tissue, peritubular myoid cells, Leydig cells, Sertoli cells, and blood vessels. Statistical analysis showed significant differences in AR expression in peritubular myoid cells and Sertoli cells (P [Open Vet J 2024; 14(12.000): 3474-3486]http://www.ejmanager.com/fulltextpdf.php?mno=223112androgen receptorimmunohistochemistrypgf2αwistar ratsα-sma |
| spellingShingle | Teuku Armansyah Husnurrizal Husnurrizal Sri Wahyuni Hafizuddin Hafizuddin Tongku Nizwan Siregar Amalia Sutriana Arman Sayuti Adinda Tri Syahrani Muhammad Bintang Pariansyah Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor Open Veterinary Journal androgen receptor immunohistochemistry pgf2α wistar rats α-sma |
| title | Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor |
| title_full | Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor |
| title_fullStr | Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor |
| title_full_unstemmed | Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor |
| title_short | Direct and indirect effects of PGF2α administration in male wistar rats based on increased expression of α-SMA and androgen receptor |
| title_sort | direct and indirect effects of pgf2α administration in male wistar rats based on increased expression of α sma and androgen receptor |
| topic | androgen receptor immunohistochemistry pgf2α wistar rats α-sma |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=223112 |
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