Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis

Schisandra chinensis fruit is a famous tonic traditional Chinese medicine and healthy food. The chemical components of dried fruits may vary with the initial processing methods of fresh fruits. Therefore, in this study, the influence of drying was evaluated based on targeted metabolomics analysis. T...

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Main Authors: Yan-hua Sheng, Rui Wang, Yi-kai Wang, Meng-meng Li, Fang-xin Liu, Xin Huang, Chang-bao Chen
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2022/2616122
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author Yan-hua Sheng
Rui Wang
Yi-kai Wang
Meng-meng Li
Fang-xin Liu
Xin Huang
Chang-bao Chen
author_facet Yan-hua Sheng
Rui Wang
Yi-kai Wang
Meng-meng Li
Fang-xin Liu
Xin Huang
Chang-bao Chen
author_sort Yan-hua Sheng
collection DOAJ
description Schisandra chinensis fruit is a famous tonic traditional Chinese medicine and healthy food. The chemical components of dried fruits may vary with the initial processing methods of fresh fruits. Therefore, in this study, the influence of drying was evaluated based on targeted metabolomics analysis. The ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS) was applied in determination of multicomponent including lignans, organic acids, and oligosaccharides. The fresh fruits of Schisandra chinensis were processed by natural drying in the sun, hot air drying and vacuum drying at different temperatures, and vacuum freeze drying, respectively. The contents of lignans, organic acids, and oligosaccharides in different fruit samples were quantified and statistically analyzed by partial least squares discriminant analysis (PLS-DA). The variation of totally 10 lignans, 5 organic acids, and 9 oligosaccharides with different drying process were compared. The results showed that the drying time of natural drying method was much longer than the other methods using instrument. For hot air and vacuum drying samples, most of lignans, organic acids, and oligosaccharides gradually decreased with the increase of temperature. And the appearance of fruit drying at higher temperature was dark red. At the same processing temperature, vacuum drying takes longer time than hot air drying. In addition, organic acid and oligosaccharide contents of vacuum freeze drying treated samples were higher than the other processed samples significantly. According to the targeted metabolomics analysis, the lignans, organic acids, and oligosaccharides markers were screened out for holistic quality evaluation of Schisandra chinensis fruits. In comprehensive consideration of multicomponent, drying time, fruit appearance and storage, and production feasibility, hot air drying at 50°C is more suitable for the process of Schisandra chinensis. The result is expected to provide a scientific basis for the selection of proper method for drying process of fresh Schisandra chinensis fruit.
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institution Kabale University
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language English
publishDate 2022-01-01
publisher Wiley
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spelling doaj-art-4acdc176d02d4aaa94436e1b8f4a0f8d2025-08-20T03:36:35ZengWileyJournal of Chemistry2090-90712022-01-01202210.1155/2022/2616122Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics AnalysisYan-hua Sheng0Rui Wang1Yi-kai Wang2Meng-meng Li3Fang-xin Liu4Xin Huang5Chang-bao Chen6Jilin Ginseng AcademyJilin Ginseng AcademyJilin Ginseng AcademyJilin Ginseng AcademyJilin Ginseng AcademyJilin Ginseng AcademyJilin Ginseng AcademySchisandra chinensis fruit is a famous tonic traditional Chinese medicine and healthy food. The chemical components of dried fruits may vary with the initial processing methods of fresh fruits. Therefore, in this study, the influence of drying was evaluated based on targeted metabolomics analysis. The ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS) was applied in determination of multicomponent including lignans, organic acids, and oligosaccharides. The fresh fruits of Schisandra chinensis were processed by natural drying in the sun, hot air drying and vacuum drying at different temperatures, and vacuum freeze drying, respectively. The contents of lignans, organic acids, and oligosaccharides in different fruit samples were quantified and statistically analyzed by partial least squares discriminant analysis (PLS-DA). The variation of totally 10 lignans, 5 organic acids, and 9 oligosaccharides with different drying process were compared. The results showed that the drying time of natural drying method was much longer than the other methods using instrument. For hot air and vacuum drying samples, most of lignans, organic acids, and oligosaccharides gradually decreased with the increase of temperature. And the appearance of fruit drying at higher temperature was dark red. At the same processing temperature, vacuum drying takes longer time than hot air drying. In addition, organic acid and oligosaccharide contents of vacuum freeze drying treated samples were higher than the other processed samples significantly. According to the targeted metabolomics analysis, the lignans, organic acids, and oligosaccharides markers were screened out for holistic quality evaluation of Schisandra chinensis fruits. In comprehensive consideration of multicomponent, drying time, fruit appearance and storage, and production feasibility, hot air drying at 50°C is more suitable for the process of Schisandra chinensis. The result is expected to provide a scientific basis for the selection of proper method for drying process of fresh Schisandra chinensis fruit.http://dx.doi.org/10.1155/2022/2616122
spellingShingle Yan-hua Sheng
Rui Wang
Yi-kai Wang
Meng-meng Li
Fang-xin Liu
Xin Huang
Chang-bao Chen
Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
Journal of Chemistry
title Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
title_full Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
title_fullStr Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
title_full_unstemmed Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
title_short Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
title_sort evaluation of multicomponent changes of schisandra chinensis fruits with different drying process by uplc qqq ms based targeted metabolomics analysis
url http://dx.doi.org/10.1155/2022/2616122
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