Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material
The use of fish rest raw material for the production of fish protein hydrolysates (FPH) through enzymatic hydrolysis has received significant interest in recent decades. Peptides derived from fish proteins are known for their enhanced bioactivity which is mainly influenced by their molecular weight....
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2024-12-01
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| author | Elissavet Kotsoni Egidijus Daukšas Grete Hansen Aas Turid Rustad Brijesh K. Tiwari Carmen Lammi Carlotta Bollati Melissa Fanzaga Lorenza d’Adduzio Janne Kristin Stangeland Janna Cropotova |
| author_facet | Elissavet Kotsoni Egidijus Daukšas Grete Hansen Aas Turid Rustad Brijesh K. Tiwari Carmen Lammi Carlotta Bollati Melissa Fanzaga Lorenza d’Adduzio Janne Kristin Stangeland Janna Cropotova |
| author_sort | Elissavet Kotsoni |
| collection | DOAJ |
| description | The use of fish rest raw material for the production of fish protein hydrolysates (FPH) through enzymatic hydrolysis has received significant interest in recent decades. Peptides derived from fish proteins are known for their enhanced bioactivity which is mainly influenced by their molecular weight. Studies have shown that novel technologies, such as high-pressure processing (HPP), can effectively modify protein structures leading to increased biological activity. This study investigated the effect of various HPP conditions on the molecular weight distribution, antioxidant activity, and dipeptidyl-peptidase IV (DPP-IV) inhibitory effect of FPH derived from a mixture of rainbow trout (<i>Oncorhynchus mykiss</i>) and Atlantic salmon (<i>Salmo salar</i>) rest raw material. Six different treatments were applied to the samples before enzymatic hydrolysis; 200 MPa × 4 min, 200 MPa × 8 min, 400 MPa × 4 min, 400 MPa × 8 min, 600 MPa × 4 min, and 600 MPa × 8 min. The antioxidant and DPP-IV inhibitory effects of the extracted FPH were measured both in vitro and at cellular level utilizing human intestinal Caco-2 cells. The results indicated that low and moderate pressures (200 and 400 MPa) increased the proportion of larger peptides (2–5 kDa) in the obtained FPH, while treatment at 600 MPa × 4 min resulted in a higher proportion of smaller peptides (1–2 kDa). Furthermore, HPP led to the formation of peptides that demonstrated increased antioxidant activity in Caco-2 cells compared to the control, whereas their potential antidiabetic activity remained unaffected. |
| format | Article |
| id | doaj-art-4abb048cf8e54c81a545a2d6904ff126 |
| institution | DOAJ |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-4abb048cf8e54c81a545a2d6904ff1262025-08-20T02:57:26ZengMDPI AGMarine Drugs1660-33972024-12-01221256810.3390/md22120568Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw MaterialElissavet Kotsoni0Egidijus Daukšas1Grete Hansen Aas2Turid Rustad3Brijesh K. Tiwari4Carmen Lammi5Carlotta Bollati6Melissa Fanzaga7Lorenza d’Adduzio8Janne Kristin Stangeland9Janna Cropotova10Department of Biological Sciences Ålesund, Norwegian University of Science and Technology, 6009 Ålesund, NorwayDepartment of Biological Sciences Ålesund, Norwegian University of Science and Technology, 6009 Ålesund, NorwayDepartment of Biological Sciences Ålesund, Norwegian University of Science and Technology, 6009 Ålesund, NorwayDepartment of Biotechnology and Food Science, Norwegian University of Science and Technology, 7034 Trondheim, NorwayFood Chemistry and Technology Department, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, IrelandDepartment of Pharmaceutical Sciences, Università degli Studi di Milano, Via Luigi Mangiagalli 25, 20133 Milano, ItalyDepartment of Pharmaceutical Sciences, Università degli Studi di Milano, Via Luigi Mangiagalli 25, 20133 Milano, ItalyDepartment of Pharmaceutical Sciences, Università degli Studi di Milano, Via Luigi Mangiagalli 25, 20133 Milano, ItalyDepartment of Pharmaceutical Sciences, Università degli Studi di Milano, Via Luigi Mangiagalli 25, 20133 Milano, ItalyMøreforsking AS, Borgundvegen 340, 6009 Ålesund, NorwayDepartment of Biological Sciences Ålesund, Norwegian University of Science and Technology, 6009 Ålesund, NorwayThe use of fish rest raw material for the production of fish protein hydrolysates (FPH) through enzymatic hydrolysis has received significant interest in recent decades. Peptides derived from fish proteins are known for their enhanced bioactivity which is mainly influenced by their molecular weight. Studies have shown that novel technologies, such as high-pressure processing (HPP), can effectively modify protein structures leading to increased biological activity. This study investigated the effect of various HPP conditions on the molecular weight distribution, antioxidant activity, and dipeptidyl-peptidase IV (DPP-IV) inhibitory effect of FPH derived from a mixture of rainbow trout (<i>Oncorhynchus mykiss</i>) and Atlantic salmon (<i>Salmo salar</i>) rest raw material. Six different treatments were applied to the samples before enzymatic hydrolysis; 200 MPa × 4 min, 200 MPa × 8 min, 400 MPa × 4 min, 400 MPa × 8 min, 600 MPa × 4 min, and 600 MPa × 8 min. The antioxidant and DPP-IV inhibitory effects of the extracted FPH were measured both in vitro and at cellular level utilizing human intestinal Caco-2 cells. The results indicated that low and moderate pressures (200 and 400 MPa) increased the proportion of larger peptides (2–5 kDa) in the obtained FPH, while treatment at 600 MPa × 4 min resulted in a higher proportion of smaller peptides (1–2 kDa). Furthermore, HPP led to the formation of peptides that demonstrated increased antioxidant activity in Caco-2 cells compared to the control, whereas their potential antidiabetic activity remained unaffected.https://www.mdpi.com/1660-3397/22/12/568innovative technologiesfish side streamsmolecular weight distributionbioactive peptidesCaco-2 cells |
| spellingShingle | Elissavet Kotsoni Egidijus Daukšas Grete Hansen Aas Turid Rustad Brijesh K. Tiwari Carmen Lammi Carlotta Bollati Melissa Fanzaga Lorenza d’Adduzio Janne Kristin Stangeland Janna Cropotova Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material Marine Drugs innovative technologies fish side streams molecular weight distribution bioactive peptides Caco-2 cells |
| title | Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material |
| title_full | Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material |
| title_fullStr | Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material |
| title_full_unstemmed | Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material |
| title_short | Antioxidant Activity and DPP-IV Inhibitory Effect of Fish Protein Hydrolysates Obtained from High-Pressure Pretreated Mixture of Rainbow Trout (<i>Oncorhynchus mykiss</i>) and Atlantic Salmon (<i>Salmo salar</i>) Rest Raw Material |
| title_sort | antioxidant activity and dpp iv inhibitory effect of fish protein hydrolysates obtained from high pressure pretreated mixture of rainbow trout i oncorhynchus mykiss i and atlantic salmon i salmo salar i rest raw material |
| topic | innovative technologies fish side streams molecular weight distribution bioactive peptides Caco-2 cells |
| url | https://www.mdpi.com/1660-3397/22/12/568 |
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