Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice.
Congenital hydrocephalus (CH) is a life-threatening medical condition in which excessive accumulation of CSF leads to ventricular expansion and increased intracranial pressure. Stenosis (blockage) of the Sylvian aqueduct (Aq; the narrow passageway that connects the third and fourth ventricles) is a...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029041&type=printable |
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| author | Kristie Lee Jacqueline Tan Michael B Morris Karine Rizzoti James Hughes Pike See Cheah Fernando Felquer Xuan Liu Sandra Piltz Robin Lovell-Badge Paul Q Thomas |
| author_facet | Kristie Lee Jacqueline Tan Michael B Morris Karine Rizzoti James Hughes Pike See Cheah Fernando Felquer Xuan Liu Sandra Piltz Robin Lovell-Badge Paul Q Thomas |
| author_sort | Kristie Lee |
| collection | DOAJ |
| description | Congenital hydrocephalus (CH) is a life-threatening medical condition in which excessive accumulation of CSF leads to ventricular expansion and increased intracranial pressure. Stenosis (blockage) of the Sylvian aqueduct (Aq; the narrow passageway that connects the third and fourth ventricles) is a common form of CH in humans, although the genetic basis of this condition is unknown. Mouse models of CH indicate that Aq stenosis is associated with abnormal development of the subcommmissural organ (SCO) a small secretory organ located at the dorsal midline of the caudal diencephalon. Glycoproteins secreted by the SCO generate Reissner's fibre (RF), a thread-like structure that descends into the Aq and is thought to maintain its patency. However, despite the importance of SCO function in CSF homeostasis, the genetic program that controls SCO development is poorly understood. Here, we show that the X-linked transcription factor SOX3 is expressed in the murine SCO throughout its development and in the mature organ. Importantly, overexpression of Sox3 in the dorsal diencephalic midline of transgenic mice induces CH via a dose-dependent mechanism. Histological, gene expression and cellular proliferation studies indicate that Sox3 overexpression disrupts the development of the SCO primordium through inhibition of diencephalic roof plate identity without inducing programmed cell death. This study provides further evidence that SCO function is essential for the prevention of hydrocephalus and indicates that overexpression of Sox3 in the dorsal midline alters progenitor cell differentiation in a dose-dependent manner. |
| format | Article |
| id | doaj-art-4ab0c3c13cf749cf9ccda5094c4b1a50 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4ab0c3c13cf749cf9ccda5094c4b1a502025-08-20T02:05:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2904110.1371/journal.pone.0029041Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice.Kristie LeeJacqueline TanMichael B MorrisKarine RizzotiJames HughesPike See CheahFernando FelquerXuan LiuSandra PiltzRobin Lovell-BadgePaul Q ThomasCongenital hydrocephalus (CH) is a life-threatening medical condition in which excessive accumulation of CSF leads to ventricular expansion and increased intracranial pressure. Stenosis (blockage) of the Sylvian aqueduct (Aq; the narrow passageway that connects the third and fourth ventricles) is a common form of CH in humans, although the genetic basis of this condition is unknown. Mouse models of CH indicate that Aq stenosis is associated with abnormal development of the subcommmissural organ (SCO) a small secretory organ located at the dorsal midline of the caudal diencephalon. Glycoproteins secreted by the SCO generate Reissner's fibre (RF), a thread-like structure that descends into the Aq and is thought to maintain its patency. However, despite the importance of SCO function in CSF homeostasis, the genetic program that controls SCO development is poorly understood. Here, we show that the X-linked transcription factor SOX3 is expressed in the murine SCO throughout its development and in the mature organ. Importantly, overexpression of Sox3 in the dorsal diencephalic midline of transgenic mice induces CH via a dose-dependent mechanism. Histological, gene expression and cellular proliferation studies indicate that Sox3 overexpression disrupts the development of the SCO primordium through inhibition of diencephalic roof plate identity without inducing programmed cell death. This study provides further evidence that SCO function is essential for the prevention of hydrocephalus and indicates that overexpression of Sox3 in the dorsal midline alters progenitor cell differentiation in a dose-dependent manner.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029041&type=printable |
| spellingShingle | Kristie Lee Jacqueline Tan Michael B Morris Karine Rizzoti James Hughes Pike See Cheah Fernando Felquer Xuan Liu Sandra Piltz Robin Lovell-Badge Paul Q Thomas Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. PLoS ONE |
| title | Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. |
| title_full | Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. |
| title_fullStr | Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. |
| title_full_unstemmed | Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. |
| title_short | Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice. |
| title_sort | congenital hydrocephalus and abnormal subcommissural organ development in sox3 transgenic mice |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029041&type=printable |
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