Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams

The interaction of radiation with biological tissues may cause some damage. To quantify it, studying cells in vitro is one methodology for analyzing dose deposition in biological tissues because once exposed to radiation, different methods can quantify the biological damage. However, biological tiss...

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Main Authors: Rafael Guimarães Malanga, Thatiane Alves Pianoschi, Cassiana Viccari, Henrique Trombini, Franciele Faccio Busatto, Dinara Jaqueline Moura, Mirko Salomon Alva-Sánchez
Format: Article
Language:English
Published: Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR) 2023-04-01
Series:Brazilian Journal of Radiation Sciences
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Online Access:https://bjrs.org.br/revista/index.php/REVISTA/article/view/2210
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author Rafael Guimarães Malanga
Thatiane Alves Pianoschi
Cassiana Viccari
Henrique Trombini
Franciele Faccio Busatto
Dinara Jaqueline Moura
Mirko Salomon Alva-Sánchez
author_facet Rafael Guimarães Malanga
Thatiane Alves Pianoschi
Cassiana Viccari
Henrique Trombini
Franciele Faccio Busatto
Dinara Jaqueline Moura
Mirko Salomon Alva-Sánchez
author_sort Rafael Guimarães Malanga
collection DOAJ
description The interaction of radiation with biological tissues may cause some damage. To quantify it, studying cells in vitro is one methodology for analyzing dose deposition in biological tissues because once exposed to radiation, different methods can quantify the biological damage. However, biological tissue culture exposure forms mostly employ high-energy beams (MeV). Thus, this study aims to characterize the radiation field from X-ray equipment using thermoluminescent dosimeters (TLD), to establish an in vitro irradiation protocol of breast cancer and glioblastoma cells for low energies. First, the central axis alignment test was performed to ensure the equipment followed the Normativa IN 90. Then the variation of radiation intensity was analyzed for a 5 x 5 cm² field at distances between 30 and 90 cm from the focal point to the detector. Subsequently, TLD immersed in breast cancer and glioblastoma cellular media were irradiated in a 106 kV and 71 mAs beam to evaluate the dose in cellular media. Simulations were performed with the PENELOPE code to compare with experimental results. The result of the central axis alignment showed that the equipment complies with the current Normativa. The dose distributions for the evaluated distances were more homogeneous for the 40 cm distance, with a standard deviation of 1.7% and 0.9% of the distributions obtained with the TLD and simulation, respectively. Thus, the irradiation field for low energy beams was characterized for a 5 x 5 cm² field for 106 kV and 71 mAs beams at a DFS of 40 cm.
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issn 2319-0612
language English
publishDate 2023-04-01
publisher Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)
record_format Article
series Brazilian Journal of Radiation Sciences
spelling doaj-art-4aaa1258ab5d4dac8eb1881afdcea99d2025-08-20T03:30:25ZengBrazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)Brazilian Journal of Radiation Sciences2319-06122023-04-01111A (Suppl.)01161828Radiation Field Characterization for Cellular Irradiation: Application for Low Energy BeamsRafael Guimarães Malanga0Thatiane Alves Pianoschi1Cassiana Viccari2https://orcid.org/0000-0003-1690-3493Henrique Trombini3Franciele Faccio Busatto4Dinara Jaqueline Moura5Mirko Salomon Alva-Sánchez6https://orcid.org/0000-0003-3936-3629Universidade Federal de Ciências de Porto Alegre (UFCSPA)Federal University of Health Sciences of Porto AlegreUniversity of São PauloFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreThe interaction of radiation with biological tissues may cause some damage. To quantify it, studying cells in vitro is one methodology for analyzing dose deposition in biological tissues because once exposed to radiation, different methods can quantify the biological damage. However, biological tissue culture exposure forms mostly employ high-energy beams (MeV). Thus, this study aims to characterize the radiation field from X-ray equipment using thermoluminescent dosimeters (TLD), to establish an in vitro irradiation protocol of breast cancer and glioblastoma cells for low energies. First, the central axis alignment test was performed to ensure the equipment followed the Normativa IN 90. Then the variation of radiation intensity was analyzed for a 5 x 5 cm² field at distances between 30 and 90 cm from the focal point to the detector. Subsequently, TLD immersed in breast cancer and glioblastoma cellular media were irradiated in a 106 kV and 71 mAs beam to evaluate the dose in cellular media. Simulations were performed with the PENELOPE code to compare with experimental results. The result of the central axis alignment showed that the equipment complies with the current Normativa. The dose distributions for the evaluated distances were more homogeneous for the 40 cm distance, with a standard deviation of 1.7% and 0.9% of the distributions obtained with the TLD and simulation, respectively. Thus, the irradiation field for low energy beams was characterized for a 5 x 5 cm² field for 106 kV and 71 mAs beams at a DFS of 40 cm.https://bjrs.org.br/revista/index.php/REVISTA/article/view/2210irradiation fieldlow energy beamsin vitro cells
spellingShingle Rafael Guimarães Malanga
Thatiane Alves Pianoschi
Cassiana Viccari
Henrique Trombini
Franciele Faccio Busatto
Dinara Jaqueline Moura
Mirko Salomon Alva-Sánchez
Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
Brazilian Journal of Radiation Sciences
irradiation field
low energy beams
in vitro cells
title Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
title_full Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
title_fullStr Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
title_full_unstemmed Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
title_short Radiation Field Characterization for Cellular Irradiation: Application for Low Energy Beams
title_sort radiation field characterization for cellular irradiation application for low energy beams
topic irradiation field
low energy beams
in vitro cells
url https://bjrs.org.br/revista/index.php/REVISTA/article/view/2210
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AT henriquetrombini radiationfieldcharacterizationforcellularirradiationapplicationforlowenergybeams
AT francielefacciobusatto radiationfieldcharacterizationforcellularirradiationapplicationforlowenergybeams
AT dinarajaquelinemoura radiationfieldcharacterizationforcellularirradiationapplicationforlowenergybeams
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