Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells
Heavy metals have serious negative effects on various aquatic organisms, and therefore rapid and accurate ecotoxicological assessments of heavy metals are necessary. Fish-derived cells sensitive to heavy metals have been used as valuable tools for ecotoxicological assessments. However, this method r...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/14/9/1119 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850260516578000896 |
|---|---|
| author | Yun Haeng Lee Myeong Uk Kuk Ji Ho Park Hojun Lee Haneur Lee Moon Kyoung So Jee Hee Yoon Yoo Jin Lee Duyeol Kim Byeonghyeon So Minseon Kim Jihae Park Taejun Han Joon Tae Park |
| author_facet | Yun Haeng Lee Myeong Uk Kuk Ji Ho Park Hojun Lee Haneur Lee Moon Kyoung So Jee Hee Yoon Yoo Jin Lee Duyeol Kim Byeonghyeon So Minseon Kim Jihae Park Taejun Han Joon Tae Park |
| author_sort | Yun Haeng Lee |
| collection | DOAJ |
| description | Heavy metals have serious negative effects on various aquatic organisms, and therefore rapid and accurate ecotoxicological assessments of heavy metals are necessary. Fish-derived cells sensitive to heavy metals have been used as valuable tools for ecotoxicological assessments. However, this method requires a minimum toxicity treatment time of 96 h, which limits its use when rapid ecotoxicological assessments are required or ecotoxicological assessments of a large number of toxicants are performed. In this study, these limitations were overcome by adjusting parameters including the concentration of fetal bovine serum (FBS) in the medium and the treatment time of the toxicant. Specifically, we found that the maximum time for fish cells to remain unstarved was 6 h when using a medium containing 1% FBS. We applied both parameters to the ecotoxicological assessment (using a medium containing 1% FBS for the toxicity assessment and treating the toxicant for only 6 h). Surprisingly, these adjusted parameters allowed us to obtain faster and more accurate data than the traditional assessment. This improvement was due to the new assessment conditions that minimized the possibility that the growth-inducing effects of nutrients present in excess in the medium could interfere with the cellular response to the toxicant. The accuracy of this assessment was not limited to measuring the toxicity of heavy metals. In conclusion, we have established an ecotoxicity assessment that can generate rapid and accurate data on heavy metals. This new platform will become the cornerstone of rapid and accurate ecotoxicity assessments of heavy metals. |
| format | Article |
| id | doaj-art-4aa5812b16664ea1b98ac2a0d91b04cd |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-4aa5812b16664ea1b98ac2a0d91b04cd2025-08-20T01:55:37ZengMDPI AGLife2075-17292024-09-01149111910.3390/life14091119Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> CellsYun Haeng Lee0Myeong Uk Kuk1Ji Ho Park2Hojun Lee3Haneur Lee4Moon Kyoung So5Jee Hee Yoon6Yoo Jin Lee7Duyeol Kim8Byeonghyeon So9Minseon Kim10Jihae Park11Taejun Han12Joon Tae Park13Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaBio Environmental Science and Technology (BEST) Lab, Ghent University Global Campus, 119-5, Songdomunhwa-ro, Incheon 21985, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaCenter for Environmental and Energy Research, Ghent University Global Campus, 119-5, Songdomunhwa-ro, Incheon 21985, Republic of KoreaBio Environmental Science and Technology (BEST) Lab, Ghent University Global Campus, 119-5, Songdomunhwa-ro, Incheon 21985, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaHeavy metals have serious negative effects on various aquatic organisms, and therefore rapid and accurate ecotoxicological assessments of heavy metals are necessary. Fish-derived cells sensitive to heavy metals have been used as valuable tools for ecotoxicological assessments. However, this method requires a minimum toxicity treatment time of 96 h, which limits its use when rapid ecotoxicological assessments are required or ecotoxicological assessments of a large number of toxicants are performed. In this study, these limitations were overcome by adjusting parameters including the concentration of fetal bovine serum (FBS) in the medium and the treatment time of the toxicant. Specifically, we found that the maximum time for fish cells to remain unstarved was 6 h when using a medium containing 1% FBS. We applied both parameters to the ecotoxicological assessment (using a medium containing 1% FBS for the toxicity assessment and treating the toxicant for only 6 h). Surprisingly, these adjusted parameters allowed us to obtain faster and more accurate data than the traditional assessment. This improvement was due to the new assessment conditions that minimized the possibility that the growth-inducing effects of nutrients present in excess in the medium could interfere with the cellular response to the toxicant. The accuracy of this assessment was not limited to measuring the toxicity of heavy metals. In conclusion, we have established an ecotoxicity assessment that can generate rapid and accurate data on heavy metals. This new platform will become the cornerstone of rapid and accurate ecotoxicity assessments of heavy metals.https://www.mdpi.com/2075-1729/14/9/1119rapid ecotoxicological assessmentaccurate ecotoxicological assessment<i>Cyprinus carpio</i> |
| spellingShingle | Yun Haeng Lee Myeong Uk Kuk Ji Ho Park Hojun Lee Haneur Lee Moon Kyoung So Jee Hee Yoon Yoo Jin Lee Duyeol Kim Byeonghyeon So Minseon Kim Jihae Park Taejun Han Joon Tae Park Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells Life rapid ecotoxicological assessment accurate ecotoxicological assessment <i>Cyprinus carpio</i> |
| title | Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells |
| title_full | Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells |
| title_fullStr | Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells |
| title_full_unstemmed | Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells |
| title_short | Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using <i>Cyprinus carpio</i> Cells |
| title_sort | rapid and accurate ecotoxicological assessment of heavy metals using i cyprinus carpio i cells |
| topic | rapid ecotoxicological assessment accurate ecotoxicological assessment <i>Cyprinus carpio</i> |
| url | https://www.mdpi.com/2075-1729/14/9/1119 |
| work_keys_str_mv | AT yunhaenglee rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT myeongukkuk rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT jihopark rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT hojunlee rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT haneurlee rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT moonkyoungso rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT jeeheeyoon rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT yoojinlee rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT duyeolkim rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT byeonghyeonso rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT minseonkim rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT jihaepark rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT taejunhan rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells AT joontaepark rapidandaccurateecotoxicologicalassessmentofheavymetalsusingicyprinuscarpioicells |