The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis
Introduction: Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is one of the leading causes of death in the world. The resource constraints make it difficult to diagnose and monitor the cases of MDR-TB. GeneXpert is a recognized tool used to diagnose the patients of pulmonary tuberculosis in...
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The Journal of Infection in Developing Countries
2022-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/14990 |
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| author | Azhar Iqbal Muhammad Shafique Muhammad Asif Zahoor Saima Muzammil Zeeshan Nawaz Abdul Jabbar Mohsin Khurshid Riaz Hussain Md. Akhttarul Islam Ahmad Almatroudi Khaled Saleh Allemailem Muhammad Hidayat Rasool Bilal Aslam |
| author_facet | Azhar Iqbal Muhammad Shafique Muhammad Asif Zahoor Saima Muzammil Zeeshan Nawaz Abdul Jabbar Mohsin Khurshid Riaz Hussain Md. Akhttarul Islam Ahmad Almatroudi Khaled Saleh Allemailem Muhammad Hidayat Rasool Bilal Aslam |
| author_sort | Azhar Iqbal |
| collection | DOAJ |
| description |
Introduction: Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is one of the leading causes of death in the world. The resource constraints make it difficult to diagnose and monitor the cases of MDR-TB. GeneXpert is a recognized tool used to diagnose the patients of pulmonary tuberculosis in clinical settings across the globe.
Methodology: The present one-year cross-sectional study was conducted to estimate the occurrence of MDR-TB in patients with pulmonary TB. A total of 1000 patients suspected of pulmonary tuberculosis were included in this study. A random convenient sampling technique was done to collect the sputum samples (twice) from the patients. Samples were processed for the detection of Mycobacterium tuberculosis using conventional detection methods like the Ziehl Nelson staining method and fluorescent microscopy. Additionally, Cepheid GeneXpert was used for molecular detection of MDR-TB in smear-positive samples of pulmonary tuberculosis by amplifying the rifampicin resistance determining region (RRDR; rpoB gene). All the tests were performed in the biosafety level III lab of District Headquarters Hospital Nankana Sahib.
Results: It was observed that 103 (10.3%) individuals were diagnosed as positive for tuberculosis among 1000 patients. Among these 103 TB positive cases, there were 11 (10.7%) patients diagnosed with rifampicin resistance gene (RR-Gene) of Mycobacterium tuberculosis.
Conclusions: Overall findings of the study showed that MDR-TB is prevalent in pulmonary TB patients and GeneXpert is the most sensitive technique for early diagnosis of the disease, which may be very helpful in the treatment and control of this public health menace in low and middle-income countries.
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| format | Article |
| id | doaj-art-4a963ffd02a94dfd878d9bb40a24b8f8 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-4a963ffd02a94dfd878d9bb40a24b8f82025-08-20T02:14:11ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-04-01160410.3855/jidc.14990The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosisAzhar Iqbal0Muhammad Shafique1Muhammad Asif Zahoor2Saima Muzammil3Zeeshan Nawaz4Abdul Jabbar5Mohsin Khurshid6Riaz Hussain7Md. Akhttarul Islam8Ahmad Almatroudi9Khaled Saleh Allemailem10Muhammad Hidayat Rasool11Bilal Aslam12Department of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Clinical Medicine, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Punjab, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanUniversity College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, PakistanStatistics Discipline, Science, Engineering and Technology School, Khulna University Khulna, BangladeshDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi ArabiaDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, Pakistan Introduction: Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is one of the leading causes of death in the world. The resource constraints make it difficult to diagnose and monitor the cases of MDR-TB. GeneXpert is a recognized tool used to diagnose the patients of pulmonary tuberculosis in clinical settings across the globe. Methodology: The present one-year cross-sectional study was conducted to estimate the occurrence of MDR-TB in patients with pulmonary TB. A total of 1000 patients suspected of pulmonary tuberculosis were included in this study. A random convenient sampling technique was done to collect the sputum samples (twice) from the patients. Samples were processed for the detection of Mycobacterium tuberculosis using conventional detection methods like the Ziehl Nelson staining method and fluorescent microscopy. Additionally, Cepheid GeneXpert was used for molecular detection of MDR-TB in smear-positive samples of pulmonary tuberculosis by amplifying the rifampicin resistance determining region (RRDR; rpoB gene). All the tests were performed in the biosafety level III lab of District Headquarters Hospital Nankana Sahib. Results: It was observed that 103 (10.3%) individuals were diagnosed as positive for tuberculosis among 1000 patients. Among these 103 TB positive cases, there were 11 (10.7%) patients diagnosed with rifampicin resistance gene (RR-Gene) of Mycobacterium tuberculosis. Conclusions: Overall findings of the study showed that MDR-TB is prevalent in pulmonary TB patients and GeneXpert is the most sensitive technique for early diagnosis of the disease, which may be very helpful in the treatment and control of this public health menace in low and middle-income countries. https://jidc.org/index.php/journal/article/view/14990TuberculosisMDRTBrifampicinfluorescent microscopyPakistan |
| spellingShingle | Azhar Iqbal Muhammad Shafique Muhammad Asif Zahoor Saima Muzammil Zeeshan Nawaz Abdul Jabbar Mohsin Khurshid Riaz Hussain Md. Akhttarul Islam Ahmad Almatroudi Khaled Saleh Allemailem Muhammad Hidayat Rasool Bilal Aslam The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis Journal of Infection in Developing Countries Tuberculosis MDR TB rifampicin fluorescent microscopy Pakistan |
| title | The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| title_full | The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| title_fullStr | The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| title_full_unstemmed | The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| title_short | The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| title_sort | occurrence of multidrug resistant mycobacterium tuberculosis from patients of pulmonary tuberculosis |
| topic | Tuberculosis MDR TB rifampicin fluorescent microscopy Pakistan |
| url | https://jidc.org/index.php/journal/article/view/14990 |
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