Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high thr...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-05-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001344&type=printable |
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| author | Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik |
| author_facet | Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik |
| author_sort | Nicholas Renzette |
| collection | DOAJ |
| description | Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus. |
| format | Article |
| id | doaj-art-4a88997ffaad481f8da0b957136ece39 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2011-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-4a88997ffaad481f8da0b957136ece392025-08-20T03:10:20ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-05-0175e100134410.1371/journal.ppat.1001344Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.Nicholas RenzetteBornali BhattacharjeeJeffrey D JensenLaura GibsonTimothy F KowalikResearch has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001344&type=printable |
| spellingShingle | Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. PLoS Pathogens |
| title | Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. |
| title_full | Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. |
| title_fullStr | Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. |
| title_full_unstemmed | Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. |
| title_short | Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. |
| title_sort | extensive genome wide variability of human cytomegalovirus in congenitally infected infants |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001344&type=printable |
| work_keys_str_mv | AT nicholasrenzette extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT bornalibhattacharjee extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT jeffreydjensen extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT lauragibson extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT timothyfkowalik extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants |