Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI
Abstract Duchenne muscular dystrophy (DMD) is a genetic muscular disease and is the most common type of muscular dystrophy in Japan. Noninvasive magnetic resonance imaging (MRI) can be used for follow-up evaluation of myositis and muscular dystrophy, including DMD and inflammation is evaluated based...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | npj Imaging |
| Online Access: | https://doi.org/10.1038/s44303-024-00058-8 |
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| author | Hinako Eto Masaharu Murata Takahito Kawano Yoko Tachibana Abdelazim Elsayed Elhelaly Yoshifumi Noda Hiroki Kato Masayuki Matsuo Fuminori Hyodo |
| author_facet | Hinako Eto Masaharu Murata Takahito Kawano Yoko Tachibana Abdelazim Elsayed Elhelaly Yoshifumi Noda Hiroki Kato Masayuki Matsuo Fuminori Hyodo |
| author_sort | Hinako Eto |
| collection | DOAJ |
| description | Abstract Duchenne muscular dystrophy (DMD) is a genetic muscular disease and is the most common type of muscular dystrophy in Japan. Noninvasive magnetic resonance imaging (MRI) can be used for follow-up evaluation of myositis and muscular dystrophy, including DMD and inflammation is evaluated based on the increased muscle water as evaluated by T2-weighted MR images. However, in MDM, the redox status has not been evaluated non-invasively during the disease progression. We assessed the inflammation via the redox status in experimental animal disease models using in vivo dynamic nuclear polarization MRI (DNP-MRI) with a redox probe. The current study aimed to evaluate the skeletal muscle of mdx mice, a DMD model, in which muscle fiber necrosis, inflammation, and muscle regeneration were chronically repeated. Results showed that the reduction rate of Carbamoyl-PROXYL (CmP), one of the redox probes, radicals in mdx mice increased compared with that in normal mice. In vitro, more mitochondria or macrophages enhanced the radical form decay reaction by reducing CmP. Due to muscle fiber damage, the mdx mice had a lower mitochondrial concentration in the gastrocnemius muscle than the normal mice. However, the in vivo DNP-MRI results strongly reflected the increased reduction of CmP radicals by macrophages. In conclusion, in vivo DNP-MRI, a noninvasive imaging method is useful for locally evaluating skeletal muscle inflammation. |
| format | Article |
| id | doaj-art-4a84bbf92b0e4277b76daca51cf33645 |
| institution | OA Journals |
| issn | 2948-197X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| series | npj Imaging |
| spelling | doaj-art-4a84bbf92b0e4277b76daca51cf336452025-08-20T02:20:41ZengNature Portfolionpj Imaging2948-197X2024-12-012111010.1038/s44303-024-00058-8Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRIHinako Eto0Masaharu Murata1Takahito Kawano2Yoko Tachibana3Abdelazim Elsayed Elhelaly4Yoshifumi Noda5Hiroki Kato6Masayuki Matsuo7Fuminori Hyodo8Center for Advanced Medical Open Innovation, Kyushu UniversityCenter for Advanced Medical Open Innovation, Kyushu UniversityCenter for Advanced Medical Open Innovation, Kyushu UniversityCenter for Advanced Medical Open Innovation, Kyushu UniversityDepartment of Radiology, Gifu UniversityDepartment of Radiology, Gifu UniversityDepartment of Radiology, Gifu UniversityDepartment of Radiology, Gifu UniversityInnovation Research Center for Quantum Medicine, Graduate School of Medicine, Gifu UniversityAbstract Duchenne muscular dystrophy (DMD) is a genetic muscular disease and is the most common type of muscular dystrophy in Japan. Noninvasive magnetic resonance imaging (MRI) can be used for follow-up evaluation of myositis and muscular dystrophy, including DMD and inflammation is evaluated based on the increased muscle water as evaluated by T2-weighted MR images. However, in MDM, the redox status has not been evaluated non-invasively during the disease progression. We assessed the inflammation via the redox status in experimental animal disease models using in vivo dynamic nuclear polarization MRI (DNP-MRI) with a redox probe. The current study aimed to evaluate the skeletal muscle of mdx mice, a DMD model, in which muscle fiber necrosis, inflammation, and muscle regeneration were chronically repeated. Results showed that the reduction rate of Carbamoyl-PROXYL (CmP), one of the redox probes, radicals in mdx mice increased compared with that in normal mice. In vitro, more mitochondria or macrophages enhanced the radical form decay reaction by reducing CmP. Due to muscle fiber damage, the mdx mice had a lower mitochondrial concentration in the gastrocnemius muscle than the normal mice. However, the in vivo DNP-MRI results strongly reflected the increased reduction of CmP radicals by macrophages. In conclusion, in vivo DNP-MRI, a noninvasive imaging method is useful for locally evaluating skeletal muscle inflammation.https://doi.org/10.1038/s44303-024-00058-8 |
| spellingShingle | Hinako Eto Masaharu Murata Takahito Kawano Yoko Tachibana Abdelazim Elsayed Elhelaly Yoshifumi Noda Hiroki Kato Masayuki Matsuo Fuminori Hyodo Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI npj Imaging |
| title | Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI |
| title_full | Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI |
| title_fullStr | Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI |
| title_full_unstemmed | Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI |
| title_short | Evaluation of the redox alteration in Duchenne muscular dystrophy model mice using in vivo DNP-MRI |
| title_sort | evaluation of the redox alteration in duchenne muscular dystrophy model mice using in vivo dnp mri |
| url | https://doi.org/10.1038/s44303-024-00058-8 |
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