Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>]
Transition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle...
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2025-01-01
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| Series: | Inorganics |
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| author | Vanja Ralić Maja D. Nešić Tanja Dučić Milutin Stepić Lela Korićanac Katarina Davalieva Marijana Petković |
| author_facet | Vanja Ralić Maja D. Nešić Tanja Dučić Milutin Stepić Lela Korićanac Katarina Davalieva Marijana Petković |
| author_sort | Vanja Ralić |
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| description | Transition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle changes caused by transition metal ions ingested by the cells. The impact of dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl<sub>2</sub>], was studied by synchrotron radiation-based Fourier transform infrared (SR FTIR) spectroscopy, a powerful tool for studying alterations in cellular components’ biochemical composition and biomolecular secondary structure on a single-cell level. A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. Although a certain level of lipid peroxidation was detectable by SR FTIR spectroscopy and confirmed by an analysis of cellular lipids by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry, the oxidative stress is not a significant mechanism by which Pd(II) expresses the effect on the HeLa cells. |
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| institution | Kabale University |
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| language | English |
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| publisher | MDPI AG |
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| spelling | doaj-art-4a83b5d8465a4c27bbf1d5b88f57c60e2025-01-24T13:35:30ZengMDPI AGInorganics2304-67402025-01-011312010.3390/inorganics13010020Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>]Vanja Ralić0Maja D. Nešić1Tanja Dučić2Milutin Stepić3Lela Korićanac4Katarina Davalieva5Marijana Petković6Center for Light-Based Research and Technologies COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaCenter for Light-Based Research and Technologies COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaALBA-CELLS Synchrotron, Cerdanyola del Vallès, 08290 Barcelona, SpainCenter for Light-Based Research and Technologies COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaResearch Centre for Genetic Engineering and Biotechnology “Georgi D Efremov”, Macedonian Academy of Sciences and Arts, 1000 Skopje, North MacedoniaCenter for Light-Based Research and Technologies COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaTransition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle changes caused by transition metal ions ingested by the cells. The impact of dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl<sub>2</sub>], was studied by synchrotron radiation-based Fourier transform infrared (SR FTIR) spectroscopy, a powerful tool for studying alterations in cellular components’ biochemical composition and biomolecular secondary structure on a single-cell level. A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. Although a certain level of lipid peroxidation was detectable by SR FTIR spectroscopy and confirmed by an analysis of cellular lipids by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry, the oxidative stress is not a significant mechanism by which Pd(II) expresses the effect on the HeLa cells.https://www.mdpi.com/2304-6740/13/1/20palladiummetallodrugstransition metal complexsynchrotron radiation Fourier transform infrared spectroscopy |
| spellingShingle | Vanja Ralić Maja D. Nešić Tanja Dučić Milutin Stepić Lela Korićanac Katarina Davalieva Marijana Petković Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] Inorganics palladium metallodrugs transition metal complex synchrotron radiation Fourier transform infrared spectroscopy |
| title | Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] |
| title_full | Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] |
| title_fullStr | Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] |
| title_full_unstemmed | Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] |
| title_short | Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl<sub>2</sub>] |
| title_sort | analysis of biomolecular changes in hela cervical cancer cell line induced by interaction with pd dach cl sub 2 sub |
| topic | palladium metallodrugs transition metal complex synchrotron radiation Fourier transform infrared spectroscopy |
| url | https://www.mdpi.com/2304-6740/13/1/20 |
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