Biological Control of Black Spot Disease in Cherry Tomato Caused by <i>Alternaria alternata</i> with <i>Bacillus velezensis</i> T3
Black spot is a major postharvest disease of cherry tomatoes, caused by <i>Alternaria alternata</i>. This causes economic losses and storage challenges, so researchers are exploring alternative methods. The biological control of fruits and vegetables using antagonistic bacteria and yeast...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/10/1700 |
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| Summary: | Black spot is a major postharvest disease of cherry tomatoes, caused by <i>Alternaria alternata</i>. This causes economic losses and storage challenges, so researchers are exploring alternative methods. The biological control of fruits and vegetables using antagonistic bacteria and yeasts is currently a research hotspot. Initially, the biological control impact of <i>Bacillus velezensis</i> T3 on cherry tomato black spot was investigated. Disease defense, scavenging reactive oxygen species, and antioxidant-related enzymes were determined during different storage periods. The relative gene expressions of these enzymes were also confirmed using RT-qPCR. The results showed that <i>B. velezensis</i> T3 reduced the incidence of black spot disease in cherry tomatoes. The growth of <i>A. alternata</i> was suppressed by <i>B. velezensis</i> T3 cell-free filtrate both <i>in vitro</i> and <i>in vivo</i>. In addition, <i>B. velezensis</i> T3 induced the activities of disease resistance-related enzymes such as polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), β-1,3-glucanase (GLU), and chitinase (CHI), and the activities of the ROS-related enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), and reduced the rate of O<sub>2</sub><sup>−</sup> production and H<sub>2</sub>O<sub>2</sub>, and MDA content of cherry tomatoes. This approach offers a promising alternative for extending shelf life, though further studies are needed to fully characterize its effects on fruit quality. |
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| ISSN: | 2304-8158 |