Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome
Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In t...
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MDPI AG
2025-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/3/241 |
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| author | Zhifei Zhang Xin Su Dun Shuo Dawei Yan Xue Pan Bangfeng Xu Minghao Yan Shuxuan Ren Qinfang Liu Chunxiu Yuan Qiaoyang Teng Zejun Li |
| author_facet | Zhifei Zhang Xin Su Dun Shuo Dawei Yan Xue Pan Bangfeng Xu Minghao Yan Shuxuan Ren Qinfang Liu Chunxiu Yuan Qiaoyang Teng Zejun Li |
| author_sort | Zhifei Zhang |
| collection | DOAJ |
| description | Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In this study, we developed a quantitative reverse transcription PCR (RT-qPCR) assay for detecting DERSV. Specific primers and a probe were designed to target a conserved region of the 3D gene. The assay demonstrated high specificity, with no cross-reactivity to other non-target duck viruses. It had a detection limit of 10<sup>2</sup> copies and a linear range from 10<sup>2</sup> to 10<sup>9</sup> copies per reaction. The assay’s efficiency was 92.59%, with a regression coefficient (R<sup>2</sup>) of 0.999. The coefficient of variation for both intra-and inter-assays was less than 2.00%. Among the 153 clinical samples collected from 2016 to 2023, the RT-qPCR detected a DERSV positive ratio of 47.06% (72/153). In conclusion, the utilization of the real-time RT-qPCR assay holds potential for the detection of DERSV in epidemiological and pathogenesis studies. |
| format | Article |
| id | doaj-art-4a503e58de9c4050be9f9be99e66d951 |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj-art-4a503e58de9c4050be9f9be99e66d9512025-08-20T02:10:25ZengMDPI AGVeterinary Sciences2306-73812025-03-0112324110.3390/vetsci12030241Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing SyndromeZhifei Zhang0Xin Su1Dun Shuo2Dawei Yan3Xue Pan4Bangfeng Xu5Minghao Yan6Shuxuan Ren7Qinfang Liu8Chunxiu Yuan9Qiaoyang Teng10Zejun Li11Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDepartment of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaDuck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In this study, we developed a quantitative reverse transcription PCR (RT-qPCR) assay for detecting DERSV. Specific primers and a probe were designed to target a conserved region of the 3D gene. The assay demonstrated high specificity, with no cross-reactivity to other non-target duck viruses. It had a detection limit of 10<sup>2</sup> copies and a linear range from 10<sup>2</sup> to 10<sup>9</sup> copies per reaction. The assay’s efficiency was 92.59%, with a regression coefficient (R<sup>2</sup>) of 0.999. The coefficient of variation for both intra-and inter-assays was less than 2.00%. Among the 153 clinical samples collected from 2016 to 2023, the RT-qPCR detected a DERSV positive ratio of 47.06% (72/153). In conclusion, the utilization of the real-time RT-qPCR assay holds potential for the detection of DERSV in epidemiological and pathogenesis studies.https://www.mdpi.com/2306-7381/12/3/241DERSVRT-qPCRTaqManspecificitysensitivity |
| spellingShingle | Zhifei Zhang Xin Su Dun Shuo Dawei Yan Xue Pan Bangfeng Xu Minghao Yan Shuxuan Ren Qinfang Liu Chunxiu Yuan Qiaoyang Teng Zejun Li Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome Veterinary Sciences DERSV RT-qPCR TaqMan specificity sensitivity |
| title | Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome |
| title_full | Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome |
| title_fullStr | Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome |
| title_full_unstemmed | Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome |
| title_short | Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome |
| title_sort | development of an rt qpcr assay for the detection of an emerging duck egg reducing syndrome |
| topic | DERSV RT-qPCR TaqMan specificity sensitivity |
| url | https://www.mdpi.com/2306-7381/12/3/241 |
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