Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429

Two new C<sub>23</sub>-steroids derivatives, cyclocitrinoic acid A (<b>1</b>) and cyclocitrinoic acid B (<b>2</b>), and a new isocoumarin metabolite, (3<i>R</i>,4<i>S</i>)-6,8-dihydroxy-3,4,5-trimethyl-7-carboxamidelisocoumarin (<b>10...

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Main Authors: Lishan Huang, Chunmei Chen, Jian Cai, Yixin Chen, Yongyan Zhu, Bin Yang, Xuefeng Zhou, Yonghong Liu, Huaming Tao
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Marine Drugs
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Online Access:https://www.mdpi.com/1660-3397/22/9/393
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author Lishan Huang
Chunmei Chen
Jian Cai
Yixin Chen
Yongyan Zhu
Bin Yang
Xuefeng Zhou
Yonghong Liu
Huaming Tao
author_facet Lishan Huang
Chunmei Chen
Jian Cai
Yixin Chen
Yongyan Zhu
Bin Yang
Xuefeng Zhou
Yonghong Liu
Huaming Tao
author_sort Lishan Huang
collection DOAJ
description Two new C<sub>23</sub>-steroids derivatives, cyclocitrinoic acid A (<b>1</b>) and cyclocitrinoic acid B (<b>2</b>), and a new isocoumarin metabolite, (3<i>R</i>,4<i>S</i>)-6,8-dihydroxy-3,4,5-trimethyl-7-carboxamidelisocoumarin (<b>10</b>), together with 12 known compounds (<b>3</b>–<b>9</b>, <b>11</b>–<b>15</b>) were isolated from the mangrove-sediment fungus <i>Penicillium</i> sp. SCSIO 41429. The structures of the new compounds were comprehensively characterized by 1D and 2D NMR, HRESIMS and ECD calculation. All isolates were evaluated for pancreatic lipase (PL) inhibitory and antioxidant activities. The biological evaluation results revealed that compounds <b>2</b>, <b>14</b> and <b>15</b> displayed weak or moderate inhibition against PL, with IC<sub>50</sub> values of 32.77, 5.15 and 2.42 µM, respectively. In addition, compounds <b>7</b>, <b>12</b> and <b>13</b> showed radical scavenging activities against DPPH, with IC<sub>50</sub> values of 64.70, 48.13, and 75.54 µM, respectively. In addition, molecular docking results indicated that these compounds had potential for PL inhibitory and antioxidant activities, which provided screening candidates for antioxidants and a reduction in obesity.
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spelling doaj-art-4a4839d04f7c41edb1d1c6d2d607d9b32025-08-20T01:55:38ZengMDPI AGMarine Drugs1660-33972024-08-0122939310.3390/md22090393Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429Lishan Huang0Chunmei Chen1Jian Cai2Yixin Chen3Yongyan Zhu4Bin Yang5Xuefeng Zhou6Yonghong Liu7Huaming Tao8Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaGuangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaGuangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaTwo new C<sub>23</sub>-steroids derivatives, cyclocitrinoic acid A (<b>1</b>) and cyclocitrinoic acid B (<b>2</b>), and a new isocoumarin metabolite, (3<i>R</i>,4<i>S</i>)-6,8-dihydroxy-3,4,5-trimethyl-7-carboxamidelisocoumarin (<b>10</b>), together with 12 known compounds (<b>3</b>–<b>9</b>, <b>11</b>–<b>15</b>) were isolated from the mangrove-sediment fungus <i>Penicillium</i> sp. SCSIO 41429. The structures of the new compounds were comprehensively characterized by 1D and 2D NMR, HRESIMS and ECD calculation. All isolates were evaluated for pancreatic lipase (PL) inhibitory and antioxidant activities. The biological evaluation results revealed that compounds <b>2</b>, <b>14</b> and <b>15</b> displayed weak or moderate inhibition against PL, with IC<sub>50</sub> values of 32.77, 5.15 and 2.42 µM, respectively. In addition, compounds <b>7</b>, <b>12</b> and <b>13</b> showed radical scavenging activities against DPPH, with IC<sub>50</sub> values of 64.70, 48.13, and 75.54 µM, respectively. In addition, molecular docking results indicated that these compounds had potential for PL inhibitory and antioxidant activities, which provided screening candidates for antioxidants and a reduction in obesity.https://www.mdpi.com/1660-3397/22/9/393C<sub>23</sub>-steroidisocoumarin<i>Penicillium</i>mangrove-sediment fungusantioxidantpancreatic lipase (PL)
spellingShingle Lishan Huang
Chunmei Chen
Jian Cai
Yixin Chen
Yongyan Zhu
Bin Yang
Xuefeng Zhou
Yonghong Liu
Huaming Tao
Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
Marine Drugs
C<sub>23</sub>-steroid
isocoumarin
<i>Penicillium</i>
mangrove-sediment fungus
antioxidant
pancreatic lipase (PL)
title Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
title_full Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
title_fullStr Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
title_full_unstemmed Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
title_short Two C<sub>23</sub>-Steroids and a New Isocoumarin Metabolite from Mangrove Sediment-Derived Fungus <i>Penicillium</i> sp. SCSIO 41429
title_sort two c sub 23 sub steroids and a new isocoumarin metabolite from mangrove sediment derived fungus i penicillium i sp scsio 41429
topic C<sub>23</sub>-steroid
isocoumarin
<i>Penicillium</i>
mangrove-sediment fungus
antioxidant
pancreatic lipase (PL)
url https://www.mdpi.com/1660-3397/22/9/393
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