Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts
The application of biomolecules extracted from plants in food and pharmaceutical industry is of great interest. In the light of that fact, our interest has been aroused to study the efficiency of chlorophormic and ethyl acetat solvents for the extraction of the main molecules from Astragalus membran...
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| Format: | Article |
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Taylor & Francis Group
2025-12-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2025.2509616 |
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| author | Mohamed El Amine Smaali Fadila Khaldi Maya Bouafia Fatima Kerroum Mohamed Abidi Mohamed Lahmadi Nassim Madi Barbara Sawicka Sheikh F. Ahmad Sabry M. Attia Wafa Zahnit Mohammed Messaoudi |
| author_facet | Mohamed El Amine Smaali Fadila Khaldi Maya Bouafia Fatima Kerroum Mohamed Abidi Mohamed Lahmadi Nassim Madi Barbara Sawicka Sheikh F. Ahmad Sabry M. Attia Wafa Zahnit Mohammed Messaoudi |
| author_sort | Mohamed El Amine Smaali |
| collection | DOAJ |
| description | The application of biomolecules extracted from plants in food and pharmaceutical industry is of great interest. In the light of that fact, our interest has been aroused to study the efficiency of chlorophormic and ethyl acetat solvents for the extraction of the main molecules from Astragalus membranaceus aerial parts (AMA) in order to evaluate the antioxidant, inhibition of α-amylase and Angiotensin-Converting Enzyme (ACE), antibacterial, cytotoxic activies and in vitro DNA-protective capacity of these extracts. Gas chromatography-mass spectrometry (GC-MS) analysis showed the presence of methyl petroselinate acid as the main compound (45, 87%) in the ethyl acetate extract (EA-AMA) and the main compound in the chloroform extract (C-AMA) was linolenic acid (16.95%). The ethyl acetate extract exhibited strong antioxidant activity with interesting IC50 values not exceeding 40 μg/mL in almost all activities. The highest in vitro activity against α-amylase and ACE activity was obtained from chloroform extract with an IC50 of 20.36 and 20.77 µg/ml, respectively. The resazurin microtiter assay was carried out to analyze the extract potential against multi drug resistant strains of clinical bacteria origin of which the extracts had powerful antibacterial activity with minimum inhibitory concentration against Pseudomonas aeruginosa (0.039 ± 0 mg/ml) and Escherichia coli (0.039 ± 0 mg/ml). The extracts had a strong protective effect of Fenton-induced DNA damage. The XTT test revealed that the extracts had weaker cytotoxicity profile against EMT6 cell line. These findings suggest that this plant has a wide range of potential applications as a food supplement, a preventative and medicinal agent. |
| format | Article |
| id | doaj-art-4a3fb1fccd3b456cbe3afe0463eb4b3a |
| institution | DOAJ |
| issn | 1094-2912 1532-2386 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj-art-4a3fb1fccd3b456cbe3afe0463eb4b3a2025-08-20T03:20:10ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862025-12-0128110.1080/10942912.2025.2509616Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal partsMohamed El Amine Smaali0Fadila Khaldi1Maya Bouafia2Fatima Kerroum3Mohamed Abidi4Mohamed Lahmadi5Nassim Madi6Barbara Sawicka7Sheikh F. Ahmad8Sabry M. Attia9Wafa Zahnit10Mohammed Messaoudi11Laboratory of Sciences and Technology of Water and Environment, University of Mohamed Cherif Messaadia, Souk-Ahras, AlgeriaLaboratory of Sciences and Technology of Water and Environment, University of Mohamed Cherif Messaadia, Souk-Ahras, AlgeriaDépartement de biologie animale, faculté de sciences de la nature et de la vie, Université Mentouri Constantine1, AlgeriaNational Center of Biotechnology Research Constantine (CRBt), Constantine, AlgeriaDépartement de biologie,Faculté des sciences de la nature et de la vie, Université de Blida 01. Laboratoire de Biologie des Systèmes Microbiens (LBSM) Ecole normale supérieur de Kouba National Center of Biotechnology Research Constantine (CRBt), Constantine, AlgeriaNational Center of Biotechnology Research Constantine (CRBt), Constantine, AlgeriaCentre de recherche en technologies agroalimentaires CRTAA, Route de targa ouzemmour campus universitaire, Bejaia, AlgeriaDepartment of Plant Production Technology and Commodities Science, University of Life Sciences in Lublin, Lublin, PolandDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Chemistry, Faculty of Sciences, University of Ferhat ABBAS Setif 1, Setif, AlgeriaNuclear Research Centre of Birine, Ain Oussera, Djelfa, AlgeriaThe application of biomolecules extracted from plants in food and pharmaceutical industry is of great interest. In the light of that fact, our interest has been aroused to study the efficiency of chlorophormic and ethyl acetat solvents for the extraction of the main molecules from Astragalus membranaceus aerial parts (AMA) in order to evaluate the antioxidant, inhibition of α-amylase and Angiotensin-Converting Enzyme (ACE), antibacterial, cytotoxic activies and in vitro DNA-protective capacity of these extracts. Gas chromatography-mass spectrometry (GC-MS) analysis showed the presence of methyl petroselinate acid as the main compound (45, 87%) in the ethyl acetate extract (EA-AMA) and the main compound in the chloroform extract (C-AMA) was linolenic acid (16.95%). The ethyl acetate extract exhibited strong antioxidant activity with interesting IC50 values not exceeding 40 μg/mL in almost all activities. The highest in vitro activity against α-amylase and ACE activity was obtained from chloroform extract with an IC50 of 20.36 and 20.77 µg/ml, respectively. The resazurin microtiter assay was carried out to analyze the extract potential against multi drug resistant strains of clinical bacteria origin of which the extracts had powerful antibacterial activity with minimum inhibitory concentration against Pseudomonas aeruginosa (0.039 ± 0 mg/ml) and Escherichia coli (0.039 ± 0 mg/ml). The extracts had a strong protective effect of Fenton-induced DNA damage. The XTT test revealed that the extracts had weaker cytotoxicity profile against EMT6 cell line. These findings suggest that this plant has a wide range of potential applications as a food supplement, a preventative and medicinal agent.https://www.tandfonline.com/doi/10.1080/10942912.2025.2509616Astragalus membranaceusaerial partschemical compositionbiological virtues |
| spellingShingle | Mohamed El Amine Smaali Fadila Khaldi Maya Bouafia Fatima Kerroum Mohamed Abidi Mohamed Lahmadi Nassim Madi Barbara Sawicka Sheikh F. Ahmad Sabry M. Attia Wafa Zahnit Mohammed Messaoudi Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts International Journal of Food Properties Astragalus membranaceus aerial parts chemical composition biological virtues |
| title | Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts |
| title_full | Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts |
| title_fullStr | Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts |
| title_full_unstemmed | Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts |
| title_short | Phytochemical composition and biological activities of extracts from Astragalus membranaceus arieal parts |
| title_sort | phytochemical composition and biological activities of extracts from astragalus membranaceus arieal parts |
| topic | Astragalus membranaceus aerial parts chemical composition biological virtues |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2025.2509616 |
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