Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations
Two chemometrics methods—principal component regression and partial least squares—were developed for simultaneous spectrophotometric estimation of ciprofloxacin and doxycycline hyclate in pharmaceutical dosage forms without any pretreatment. The UV spectra of both drugs were recorded at concentratio...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/9538435 |
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| author | Tadele Eticha Getu Kahsay Fitsum Asefa Teklebrhan Hailu Hailekiros Gebretsadik Tesfamichael Gebretsadikan Boovizhikannan Thangabalan |
| author_facet | Tadele Eticha Getu Kahsay Fitsum Asefa Teklebrhan Hailu Hailekiros Gebretsadik Tesfamichael Gebretsadikan Boovizhikannan Thangabalan |
| author_sort | Tadele Eticha |
| collection | DOAJ |
| description | Two chemometrics methods—principal component regression and partial least squares—were developed for simultaneous spectrophotometric estimation of ciprofloxacin and doxycycline hyclate in pharmaceutical dosage forms without any pretreatment. The UV spectra of both drugs were recorded at concentrations within their linear ranges between 200 and 400 nm with the intervals λ = 2 nm at 100 wavelengths in distilled water. Beer’s law was obeyed for both drugs in the concentration ranges of 1–10 μg/mL for ciprofloxacin and 5–25 μg/mL for doxycycline hyclate. Two sets of standard mixtures, 25 as a calibration set and 9 as a validation set, were prepared. The calibration models were evaluated by cross-validation and external validation over synthetic mixtures. The optimized models were successfully applied for chemometric analysis of ciprofloxacin and doxycycline hyclate in synthetic and pharmaceutical mixtures with satisfactory accuracy (recovery values from 97.50% to 101.87%) and precision (RSD < 2%). |
| format | Article |
| id | doaj-art-4a377a6aaa1d4a888f6e0107c13e1f43 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-4a377a6aaa1d4a888f6e0107c13e1f432025-02-03T01:23:19ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/95384359538435Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical FormulationsTadele Eticha0Getu Kahsay1Fitsum Asefa2Teklebrhan Hailu3Hailekiros Gebretsadik4Tesfamichael Gebretsadikan5Boovizhikannan Thangabalan6School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaTwo chemometrics methods—principal component regression and partial least squares—were developed for simultaneous spectrophotometric estimation of ciprofloxacin and doxycycline hyclate in pharmaceutical dosage forms without any pretreatment. The UV spectra of both drugs were recorded at concentrations within their linear ranges between 200 and 400 nm with the intervals λ = 2 nm at 100 wavelengths in distilled water. Beer’s law was obeyed for both drugs in the concentration ranges of 1–10 μg/mL for ciprofloxacin and 5–25 μg/mL for doxycycline hyclate. Two sets of standard mixtures, 25 as a calibration set and 9 as a validation set, were prepared. The calibration models were evaluated by cross-validation and external validation over synthetic mixtures. The optimized models were successfully applied for chemometric analysis of ciprofloxacin and doxycycline hyclate in synthetic and pharmaceutical mixtures with satisfactory accuracy (recovery values from 97.50% to 101.87%) and precision (RSD < 2%).http://dx.doi.org/10.1155/2018/9538435 |
| spellingShingle | Tadele Eticha Getu Kahsay Fitsum Asefa Teklebrhan Hailu Hailekiros Gebretsadik Tesfamichael Gebretsadikan Boovizhikannan Thangabalan Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations Journal of Analytical Methods in Chemistry |
| title | Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations |
| title_full | Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations |
| title_fullStr | Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations |
| title_full_unstemmed | Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations |
| title_short | Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations |
| title_sort | chemometric assisted spectrophotometric method for the simultaneous determination of ciprofloxacin and doxycycline hyclate in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2018/9538435 |
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