Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli
Escherichia coli is considered one of the uropathogenic bacteria with different infection symptoms representing mild illness to acute sepsis. This study aims to detect E. coli in patients with urinary infection and investigate quorum sensing genes (lux S and motA) in multi-drug resistant isolates o...
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Mustansiriyah University
2024-03-01
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| Series: | Al-Mustansiriyah Journal of Science |
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| Online Access: | https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1429 |
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| author | Hanan M. Abbas Radhouane Gdoura Mohammed F. Al-Marjani |
| author_facet | Hanan M. Abbas Radhouane Gdoura Mohammed F. Al-Marjani |
| author_sort | Hanan M. Abbas |
| collection | DOAJ |
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Escherichia coli is considered one of the uropathogenic bacteria with different infection symptoms representing mild illness to acute sepsis. This study aims to detect E. coli in patients with urinary infection and investigate quorum sensing genes (lux S and motA) in multi-drug resistant isolates of E. coli. 200 urine samples were collected from patients with urinary tract infections from several hospitals in Baghdad. The antibiotics sensitivity test showed high resistance of isolates for Ampicillin (100%), Cefazolin (97%), Trimethoprim/ Sulfamethoxazole (83%), Ceftriaxone (77%), Ceftazidime and Ciprofloxacin (70% each of them), and moderate resistance of isolates for Levofloxacin (50%), Gentamicin (47%), Cefepime (40%), while low resistance Piperacillin/ Tazobactam (33%), Cefoxitin (30%), Nitrofurantoin (17%), Imipenem (10%), Ertapenem and Amikacin (7% each of them), and Tigecycline (3%). The results showed an increase percentage of infection in females was 30% in the ages 30-44 years, whereas in ages 15-29 and more than 45 years was 17%. There was a high percentage (57.11%) of resistant isolates in females which are ages 30-44 years. While the ages more than 45 years were 66.4% and ages 15-29 were 34%. While, in males, the percentage was high in ages more than 45 years (35.25%) followed by age groups 30-44 years (31.5%) and 15-29 years (31%). The prevalence percentage for luxS and motA genes in E. coli was 100%. In conclusion, E. coli isolates were multi-drug resistant due to all isolates had quorum sensing genes. Moreover, uropathogenic of E. coli in females was more frequent than in males due to the resistance of bacteria to antibiotics.
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| format | Article |
| id | doaj-art-4a26033138a44c9da3b838be62a36a63 |
| institution | OA Journals |
| issn | 1814-635X 2521-3520 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Mustansiriyah University |
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| series | Al-Mustansiriyah Journal of Science |
| spelling | doaj-art-4a26033138a44c9da3b838be62a36a632025-08-20T02:22:49ZengMustansiriyah UniversityAl-Mustansiriyah Journal of Science1814-635X2521-35202024-03-0135110.23851/mjs.v35i1.1429Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coliHanan M. Abbas0https://orcid.org/0009-0007-8838-2136Radhouane Gdoura1https://orcid.org/0000-0003-1140-3780Mohammed F. Al-Marjani2Department of Biology, Faculty of Sciences, Sfax University, Sfax, TUNISIA.Research Laboratory of Toxicology Microbiology Environmental and Health, Sfax University, Faculty of Sciences, BP 1171, 3000, TUNISIA.Department of Biology, College of Science, Al-Mustansiriyah University, 10052 Baghdad, IRAQ. Escherichia coli is considered one of the uropathogenic bacteria with different infection symptoms representing mild illness to acute sepsis. This study aims to detect E. coli in patients with urinary infection and investigate quorum sensing genes (lux S and motA) in multi-drug resistant isolates of E. coli. 200 urine samples were collected from patients with urinary tract infections from several hospitals in Baghdad. The antibiotics sensitivity test showed high resistance of isolates for Ampicillin (100%), Cefazolin (97%), Trimethoprim/ Sulfamethoxazole (83%), Ceftriaxone (77%), Ceftazidime and Ciprofloxacin (70% each of them), and moderate resistance of isolates for Levofloxacin (50%), Gentamicin (47%), Cefepime (40%), while low resistance Piperacillin/ Tazobactam (33%), Cefoxitin (30%), Nitrofurantoin (17%), Imipenem (10%), Ertapenem and Amikacin (7% each of them), and Tigecycline (3%). The results showed an increase percentage of infection in females was 30% in the ages 30-44 years, whereas in ages 15-29 and more than 45 years was 17%. There was a high percentage (57.11%) of resistant isolates in females which are ages 30-44 years. While the ages more than 45 years were 66.4% and ages 15-29 were 34%. While, in males, the percentage was high in ages more than 45 years (35.25%) followed by age groups 30-44 years (31.5%) and 15-29 years (31%). The prevalence percentage for luxS and motA genes in E. coli was 100%. In conclusion, E. coli isolates were multi-drug resistant due to all isolates had quorum sensing genes. Moreover, uropathogenic of E. coli in females was more frequent than in males due to the resistance of bacteria to antibiotics. https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1429motAE. coliQuorum SensingluxS |
| spellingShingle | Hanan M. Abbas Radhouane Gdoura Mohammed F. Al-Marjani Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli Al-Mustansiriyah Journal of Science motA E. coli Quorum Sensing luxS |
| title | Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli |
| title_full | Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli |
| title_fullStr | Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli |
| title_full_unstemmed | Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli |
| title_short | Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli |
| title_sort | unveiling the high prevalence of antibiotic resistance and quorum sensing genes in uropathogenic escherichia coli |
| topic | motA E. coli Quorum Sensing luxS |
| url | https://mjs.uomustansiriyah.edu.iq/index.php/MJS/article/view/1429 |
| work_keys_str_mv | AT hananmabbas unveilingthehighprevalenceofantibioticresistanceandquorumsensinggenesinuropathogenicescherichiacoli AT radhouanegdoura unveilingthehighprevalenceofantibioticresistanceandquorumsensinggenesinuropathogenicescherichiacoli AT mohammedfalmarjani unveilingthehighprevalenceofantibioticresistanceandquorumsensinggenesinuropathogenicescherichiacoli |