Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants
Influenza A viruses (IAV) cause annual epidemics and occasional pandemics in humans. The most recent pandemic outbreak occurred in 2009 with H1N1pdm09. This virus, which most likely reassorted in swine before its transmission to humans, was reintroduced into the swine population and continues circul...
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2212809 |
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| author | Verónica A. Ferrando Marcel E. Friedrich Shrey Gandhi Alexander Mellmann Dörthe Masemann Anmari Christersson Darisuren Anhlan Linda Brunotte Monika Stoll Timm Harder Martin Beer Yvonne Boergeling Stephan Ludwig |
| author_facet | Verónica A. Ferrando Marcel E. Friedrich Shrey Gandhi Alexander Mellmann Dörthe Masemann Anmari Christersson Darisuren Anhlan Linda Brunotte Monika Stoll Timm Harder Martin Beer Yvonne Boergeling Stephan Ludwig |
| author_sort | Verónica A. Ferrando |
| collection | DOAJ |
| description | Influenza A viruses (IAV) cause annual epidemics and occasional pandemics in humans. The most recent pandemic outbreak occurred in 2009 with H1N1pdm09. This virus, which most likely reassorted in swine before its transmission to humans, was reintroduced into the swine population and continues circulating ever since. In order to assess its potential to cause reassortants on a cellular level, human origin H1N1pdm09 and a recent Eurasian avian-like H1N1 swine IAV were (co-)passaged in the newly generated swine lung cell line C22. Co-infection with both viruses gave rise to numerous reassortants that additionally carry different mutations which can partially be found in nature as well. Reassortment most frequently affected the PB1, PA and NA segments with the swine IAV as recipient. These reassortants reached higher titers in swine lung cells and were able to replicate in genuine human lung tissue explants ex vivo, suggesting a possible zoonotic potential. Interestingly, reassortment and mutations in the viral ribonucleoprotein complex influence the viral polymerase activity in a cell type and species-specific manner. In summary, we demonstrate reassortment promiscuity of these viruses in a novel swine lung cell model and indicate a possible zoonotic potential of the reassortants. |
| format | Article |
| id | doaj-art-4a0d20eb3cce4efea4caca01d4f9af78 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-4a0d20eb3cce4efea4caca01d4f9af782025-08-20T01:49:16ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2212809Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variantsVerónica A. Ferrando0Marcel E. Friedrich1Shrey Gandhi2Alexander Mellmann3Dörthe Masemann4Anmari Christersson5Darisuren Anhlan6Linda Brunotte7Monika Stoll8Timm Harder9Martin Beer10Yvonne Boergeling11Stephan Ludwig12Institute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyDepartment of Genetic Epidemiology, Institute of Human Genetics, Westfälische Wilhelms-University, Münster, GermanyInstitute of Hygiene Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyDepartment of Genetic Epidemiology, Institute of Human Genetics, Westfälische Wilhelms-University, Münster, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institute, Greifswald, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institute, Greifswald, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInstitute of Virology Münster, Westfälische Wilhelms-University, Münster, GermanyInfluenza A viruses (IAV) cause annual epidemics and occasional pandemics in humans. The most recent pandemic outbreak occurred in 2009 with H1N1pdm09. This virus, which most likely reassorted in swine before its transmission to humans, was reintroduced into the swine population and continues circulating ever since. In order to assess its potential to cause reassortants on a cellular level, human origin H1N1pdm09 and a recent Eurasian avian-like H1N1 swine IAV were (co-)passaged in the newly generated swine lung cell line C22. Co-infection with both viruses gave rise to numerous reassortants that additionally carry different mutations which can partially be found in nature as well. Reassortment most frequently affected the PB1, PA and NA segments with the swine IAV as recipient. These reassortants reached higher titers in swine lung cells and were able to replicate in genuine human lung tissue explants ex vivo, suggesting a possible zoonotic potential. Interestingly, reassortment and mutations in the viral ribonucleoprotein complex influence the viral polymerase activity in a cell type and species-specific manner. In summary, we demonstrate reassortment promiscuity of these viruses in a novel swine lung cell model and indicate a possible zoonotic potential of the reassortants.https://www.tandfonline.com/doi/10.1080/22221751.2023.2212809Zoonosisswineinfluenza virusreassortmentevolution |
| spellingShingle | Verónica A. Ferrando Marcel E. Friedrich Shrey Gandhi Alexander Mellmann Dörthe Masemann Anmari Christersson Darisuren Anhlan Linda Brunotte Monika Stoll Timm Harder Martin Beer Yvonne Boergeling Stephan Ludwig Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants Emerging Microbes and Infections Zoonosis swine influenza virus reassortment evolution |
| title | Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants |
| title_full | Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants |
| title_fullStr | Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants |
| title_full_unstemmed | Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants |
| title_short | Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants |
| title_sort | cell intrinsic genomic reassortment of pandemic h1n1 2009 and eurasian avian like swine influenza viruses results in potentially zoonotic variants |
| topic | Zoonosis swine influenza virus reassortment evolution |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2212809 |
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