Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish
In this investigation, collagen was successfully extracted from the skin and fins of the orange-spotted grouper, <i>Epinephelus coioides</i>, with recovery rates of 4.45% and 23.65% (dry weight basis), respectively. UV–Vis spectrophotometric analysis demonstrated distinct absorbance peak...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/10/1/13 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588477242277888 |
|---|---|
| author | Shao-Yang Hu Chen-Han Tsou Chung-Chih Tseng Tai-Wei Han Chun-Hung Liu |
| author_facet | Shao-Yang Hu Chen-Han Tsou Chung-Chih Tseng Tai-Wei Han Chun-Hung Liu |
| author_sort | Shao-Yang Hu |
| collection | DOAJ |
| description | In this investigation, collagen was successfully extracted from the skin and fins of the orange-spotted grouper, <i>Epinephelus coioides</i>, with recovery rates of 4.45% and 23.65% (dry weight basis), respectively. UV–Vis spectrophotometric analysis demonstrated distinct absorbance peaks at 222 nm and 217 nm for collagen isolated from the skin and fins, correspondingly. Both collagen sources were confirmed to be type I, characterized by the presence of two α-chains (α1 and α2), with glycine as the predominant amino acid, an absence of tryptophan, and a notable content of proline and hydroxyproline. The enzymatic hydrolysis of fin-derived collagen using pepsin yielded low-molecular-weight peptides (PHC), which were subsequently incorporated into the diet of 1.5-year-old zebrafish, either alone or in combination with glucosamine (GC), to assess their biological effects. After eight weeks of dietary supplementation, zebrafish fed PHC or the combined PHC + GC diets exhibited a significant upregulation of <i>sox9a</i> (<i>jef</i>) expression in spinal tissues, accompanied by a marked downregulation of <i>runx2</i>. Although the differences in swimming performance among the groups were not statistically significant, zebrafish that received PHC or the combined supplements demonstrated enhanced endurance compared to the control group. These results suggest that collagen sourced from grouper may have advantageous effects in supporting cartilage health in aged zebrafish. Furthermore, utilizing fish by-products for collagen extraction enhances resource efficiency and aligns with circular economy principles. |
| format | Article |
| id | doaj-art-4a086a5f254f41b99498d972537c3688 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-4a086a5f254f41b99498d972537c36882025-01-24T13:32:26ZengMDPI AGFishes2410-38882024-12-011011310.3390/fishes10010013Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged ZebrafishShao-Yang Hu0Chen-Han Tsou1Chung-Chih Tseng2Tai-Wei Han3Chun-Hung Liu4Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912301, TaiwanDepartment of Dentistry, Zuoying Armed Forces General Hospital, Kaohsiung 813204, TaiwanZuoying Armed Forces General Hospital, Kaohsiung 81342, TaiwanDepartment of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung 202301, TaiwanDepartment of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912301, TaiwanIn this investigation, collagen was successfully extracted from the skin and fins of the orange-spotted grouper, <i>Epinephelus coioides</i>, with recovery rates of 4.45% and 23.65% (dry weight basis), respectively. UV–Vis spectrophotometric analysis demonstrated distinct absorbance peaks at 222 nm and 217 nm for collagen isolated from the skin and fins, correspondingly. Both collagen sources were confirmed to be type I, characterized by the presence of two α-chains (α1 and α2), with glycine as the predominant amino acid, an absence of tryptophan, and a notable content of proline and hydroxyproline. The enzymatic hydrolysis of fin-derived collagen using pepsin yielded low-molecular-weight peptides (PHC), which were subsequently incorporated into the diet of 1.5-year-old zebrafish, either alone or in combination with glucosamine (GC), to assess their biological effects. After eight weeks of dietary supplementation, zebrafish fed PHC or the combined PHC + GC diets exhibited a significant upregulation of <i>sox9a</i> (<i>jef</i>) expression in spinal tissues, accompanied by a marked downregulation of <i>runx2</i>. Although the differences in swimming performance among the groups were not statistically significant, zebrafish that received PHC or the combined supplements demonstrated enhanced endurance compared to the control group. These results suggest that collagen sourced from grouper may have advantageous effects in supporting cartilage health in aged zebrafish. Furthermore, utilizing fish by-products for collagen extraction enhances resource efficiency and aligns with circular economy principles.https://www.mdpi.com/2410-3888/10/1/13collagenpepsinosteoblastzebrafish |
| spellingShingle | Shao-Yang Hu Chen-Han Tsou Chung-Chih Tseng Tai-Wei Han Chun-Hung Liu Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish Fishes collagen pepsin osteoblast zebrafish |
| title | Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish |
| title_full | Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish |
| title_fullStr | Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish |
| title_full_unstemmed | Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish |
| title_short | Biofunctional Characterization of Collagen from Orange-Spotted Grouper, <i>Epinephelus coioides</i>: Maintenance of Cartilage in Aged Zebrafish |
| title_sort | biofunctional characterization of collagen from orange spotted grouper i epinephelus coioides i maintenance of cartilage in aged zebrafish |
| topic | collagen pepsin osteoblast zebrafish |
| url | https://www.mdpi.com/2410-3888/10/1/13 |
| work_keys_str_mv | AT shaoyanghu biofunctionalcharacterizationofcollagenfromorangespottedgrouperiepinepheluscoioidesimaintenanceofcartilageinagedzebrafish AT chenhantsou biofunctionalcharacterizationofcollagenfromorangespottedgrouperiepinepheluscoioidesimaintenanceofcartilageinagedzebrafish AT chungchihtseng biofunctionalcharacterizationofcollagenfromorangespottedgrouperiepinepheluscoioidesimaintenanceofcartilageinagedzebrafish AT taiweihan biofunctionalcharacterizationofcollagenfromorangespottedgrouperiepinepheluscoioidesimaintenanceofcartilageinagedzebrafish AT chunhungliu biofunctionalcharacterizationofcollagenfromorangespottedgrouperiepinepheluscoioidesimaintenanceofcartilageinagedzebrafish |