Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies
The virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions....
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-03-01
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| Series: | Advanced NanoBiomed Research |
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| Online Access: | https://doi.org/10.1002/anbr.202400135 |
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| author | Reece McCoy Jeremy Treiber George G. Malliaras Alberto Salleo Róisín M. Owens |
| author_facet | Reece McCoy Jeremy Treiber George G. Malliaras Alberto Salleo Róisín M. Owens |
| author_sort | Reece McCoy |
| collection | DOAJ |
| description | The virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions. Preserving these intricacies in cell membrane‐on‐a‐chip platforms is important for retaining physiological relevance for investigating host–pathogen interactions. Consequently, a method for obtaining distinct populations of cell membrane vesicles representing the apical and basolateral membranes is presented here, in addition to the formation of their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. The apical localization of the A metalloprotease and disintegrin (ADAM10) receptor in Caco‐2 cells is shown to correlate with the increased response of the Staphylococcus aureus alpha hemolysin toxin on membrane‐on‐a‐chip platforms compared to the basolateral membrane model where the ADAM10 receptor is absent. The interaction between SLBs and the alpha hemolysin‐containing extracellular vesicles (EVs) secreted by S. aureus confirm the direct effect of toxin‐containing EVs on reducing the resistance of plasma membrane. This technique could find use in quantifying relative toxicity to the cell membrane, screening for cognate receptors and inhibitors, and probing toxin mechanism of action. |
| format | Article |
| id | doaj-art-49ee7429b13646509e6450e960a3df30 |
| institution | OA Journals |
| issn | 2699-9307 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-49ee7429b13646509e6450e960a3df302025-08-20T01:57:43ZengWiley-VCHAdvanced NanoBiomed Research2699-93072025-03-0153n/an/a10.1002/anbr.202400135Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction StudiesReece McCoy0Jeremy Treiber1George G. Malliaras2Alberto Salleo3Róisín M. Owens4Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge CB3 0AS UKDepartment of Materials Science and Engineering Stanford University Stanford CA 94305 USADepartment of Electrical Engineering University of Cambridge Cambridge CB3 0FA UKDepartment of Materials Science and Engineering Stanford University Stanford CA 94305 USADepartment of Chemical Engineering and Biotechnology University of Cambridge Cambridge CB3 0AS UKThe virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions. Preserving these intricacies in cell membrane‐on‐a‐chip platforms is important for retaining physiological relevance for investigating host–pathogen interactions. Consequently, a method for obtaining distinct populations of cell membrane vesicles representing the apical and basolateral membranes is presented here, in addition to the formation of their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. The apical localization of the A metalloprotease and disintegrin (ADAM10) receptor in Caco‐2 cells is shown to correlate with the increased response of the Staphylococcus aureus alpha hemolysin toxin on membrane‐on‐a‐chip platforms compared to the basolateral membrane model where the ADAM10 receptor is absent. The interaction between SLBs and the alpha hemolysin‐containing extracellular vesicles (EVs) secreted by S. aureus confirm the direct effect of toxin‐containing EVs on reducing the resistance of plasma membrane. This technique could find use in quantifying relative toxicity to the cell membrane, screening for cognate receptors and inhibitors, and probing toxin mechanism of action.https://doi.org/10.1002/anbr.202400135organ‐on‐a‐chippathogensreceptorssupported lipid bilayerstoxins |
| spellingShingle | Reece McCoy Jeremy Treiber George G. Malliaras Alberto Salleo Róisín M. Owens Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies Advanced NanoBiomed Research organ‐on‐a‐chip pathogens receptors supported lipid bilayers toxins |
| title | Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies |
| title_full | Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies |
| title_fullStr | Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies |
| title_full_unstemmed | Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies |
| title_short | Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies |
| title_sort | polarized intestinal cell membrane on chip for bacterial toxin interaction studies |
| topic | organ‐on‐a‐chip pathogens receptors supported lipid bilayers toxins |
| url | https://doi.org/10.1002/anbr.202400135 |
| work_keys_str_mv | AT reecemccoy polarizedintestinalcellmembraneonchipforbacterialtoxininteractionstudies AT jeremytreiber polarizedintestinalcellmembraneonchipforbacterialtoxininteractionstudies AT georgegmalliaras polarizedintestinalcellmembraneonchipforbacterialtoxininteractionstudies AT albertosalleo polarizedintestinalcellmembraneonchipforbacterialtoxininteractionstudies AT roisinmowens polarizedintestinalcellmembraneonchipforbacterialtoxininteractionstudies |