Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing
Introduction: This study explores the potential of repaglinide-loaded chitosan-based hydrogels (Hy-R1% and Hy-R3%) as multifunctional scaffolds for wound healing applications. It aims to evaluate their physicochemical properties, cytocompatibility, antibacterial activity, and in vivo healing potenti...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320425000975 |
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| author | Chou-Yi Hsu Eman Raeed Azzam Mohammed Merza Marwah Suliman Maashi Ahmed Hjazi Rekha M.M. Subhashree Ray Kapil Ghai Udaybir Singh Narges Fereydouni |
| author_facet | Chou-Yi Hsu Eman Raeed Azzam Mohammed Merza Marwah Suliman Maashi Ahmed Hjazi Rekha M.M. Subhashree Ray Kapil Ghai Udaybir Singh Narges Fereydouni |
| author_sort | Chou-Yi Hsu |
| collection | DOAJ |
| description | Introduction: This study explores the potential of repaglinide-loaded chitosan-based hydrogels (Hy-R1% and Hy-R3%) as multifunctional scaffolds for wound healing applications. It aims to evaluate their physicochemical properties, cytocompatibility, antibacterial activity, and in vivo healing potential. Methods: The hydrogels were prepared by dissolving chitosan in acetic acid, followed by the incorporation of repaglinide. In vitro assessments, including morphology, swelling, degradation, cytocompatibility, and antibacterial activity, were performed. In vivo wound healing was tested in a rat model using full-thickness wounds. Results: Hy-R3% exhibited superior properties in surface morphology, swelling behavior, and degradation rate compared to the control. It also demonstrated the highest cell viability and significant antibacterial activity against Staphylococcus aureus. In vivo, Hy-R3% accelerated wound closure, showed superior re-epithelialization, and improved collagen deposition. Histological analysis confirmed better tissue remodeling and minimal inflammation in Hy-R3%. Conclusions: Repaglinide-loaded chitosan-based hydrogels, particularly Hy-R3%, represent a promising new therapeutic approach for wound healing. The formulation demonstrated enhanced physicochemical and biological properties, including rapid wound closure and improved tissue regeneration. These findings suggest that repaglinide can be repurposed for wound healing applications, particularly for chronic and diabetic wounds, and lay the foundation for future studies on its broader regenerative potential. |
| format | Article |
| id | doaj-art-49e9de556e514b1584058a291646523e |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-49e9de556e514b1584058a291646523e2025-08-20T01:51:14ZengElsevierRegenerative Therapy2352-32042025-06-012955156210.1016/j.reth.2025.04.021Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healingChou-Yi Hsu0Eman Raeed Azzam1Mohammed Merza2Marwah Suliman Maashi3Ahmed Hjazi4Rekha M.M.5Subhashree Ray6Kapil Ghai7Udaybir Singh8Narges Fereydouni9Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 71710, TaiwanMedical Laboratory Techniques Department, College of Health and Medical Technology, University of Al-maarif, Anbar, IraqHawler Medical University, College of Pharmacy, Clinical Analysis Department, Kurdistan Region of Iraq, Erbil, Iraq; Nutrition and Dietetics Department, Tishk International University, Erbil, Iraq; Department of Pharmacy, College of Pharmacy, Knowledge University, Erbil 44001, IraqMedical Laboratory Sciences Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Regenerative Medicine Unit at King Fahad Medical Research Centre, Jeddah, Saudi ArabiaDepartment of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Chemistry and Biochemistry, School of Sciences, JAIN (Deemed to Be University), Bangalore, Karnataka, IndiaDepartment of Biochemistry, IMS and SUM Hospital, Siksha 'O' Anusandhan (Deemed to Be University), Bhubaneswar, Odisha 751003, IndiaDepartment of Chemistry, Graphic Era Hill University, Dehradun, India; Centre for Promotion of Research, Graphic Era Deemed to Be University, Dehradun, Uttarakhand 248002, IndiaCentre for Research Impact & Outcome, Chitkara University Institute of Engineering and Technology, Chitkara University, Rajpura, 140401, Punjab, IndiaNoncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran; Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran; Corresponding author. Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.Introduction: This study explores the potential of repaglinide-loaded chitosan-based hydrogels (Hy-R1% and Hy-R3%) as multifunctional scaffolds for wound healing applications. It aims to evaluate their physicochemical properties, cytocompatibility, antibacterial activity, and in vivo healing potential. Methods: The hydrogels were prepared by dissolving chitosan in acetic acid, followed by the incorporation of repaglinide. In vitro assessments, including morphology, swelling, degradation, cytocompatibility, and antibacterial activity, were performed. In vivo wound healing was tested in a rat model using full-thickness wounds. Results: Hy-R3% exhibited superior properties in surface morphology, swelling behavior, and degradation rate compared to the control. It also demonstrated the highest cell viability and significant antibacterial activity against Staphylococcus aureus. In vivo, Hy-R3% accelerated wound closure, showed superior re-epithelialization, and improved collagen deposition. Histological analysis confirmed better tissue remodeling and minimal inflammation in Hy-R3%. Conclusions: Repaglinide-loaded chitosan-based hydrogels, particularly Hy-R3%, represent a promising new therapeutic approach for wound healing. The formulation demonstrated enhanced physicochemical and biological properties, including rapid wound closure and improved tissue regeneration. These findings suggest that repaglinide can be repurposed for wound healing applications, particularly for chronic and diabetic wounds, and lay the foundation for future studies on its broader regenerative potential.http://www.sciencedirect.com/science/article/pii/S2352320425000975RepaglinideChitosan-based hydrogelWound healingAntibacterial activityTissue regenerationCytocompatibility |
| spellingShingle | Chou-Yi Hsu Eman Raeed Azzam Mohammed Merza Marwah Suliman Maashi Ahmed Hjazi Rekha M.M. Subhashree Ray Kapil Ghai Udaybir Singh Narges Fereydouni Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing Regenerative Therapy Repaglinide Chitosan-based hydrogel Wound healing Antibacterial activity Tissue regeneration Cytocompatibility |
| title | Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing |
| title_full | Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing |
| title_fullStr | Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing |
| title_full_unstemmed | Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing |
| title_short | Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing |
| title_sort | therapeutic potential of repaglinide embedded chitosan hydrogel in promoting wound healing |
| topic | Repaglinide Chitosan-based hydrogel Wound healing Antibacterial activity Tissue regeneration Cytocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2352320425000975 |
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