In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots
Abstract Colorectal cancer is the second most common cause of cancer‐related deaths worldwide and the third most common cancer overall. In this study, we investigate the anti‐colon cancer potential of phytochemically, and thermally synthesised novel green carbon dots based on Rhododendron luteum (RL...
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| Format: | Article |
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Wiley-VCH
2025-05-01
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| Online Access: | https://doi.org/10.1002/open.202400303 |
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| author | Dr. Alper Durmaz İbrahim Mizan Kahyaoğlu Erdi Can Aytar Ferdane Danışman Kalındemirtaş Esra Sert Ayşe Erol‐Bozkurt Selcan Karakuş |
| author_facet | Dr. Alper Durmaz İbrahim Mizan Kahyaoğlu Erdi Can Aytar Ferdane Danışman Kalındemirtaş Esra Sert Ayşe Erol‐Bozkurt Selcan Karakuş |
| author_sort | Dr. Alper Durmaz |
| collection | DOAJ |
| description | Abstract Colorectal cancer is the second most common cause of cancer‐related deaths worldwide and the third most common cancer overall. In this study, we investigate the anti‐colon cancer potential of phytochemically, and thermally synthesised novel green carbon dots based on Rhododendron luteum (RL‐CDs). A new synthesis method was used to produce carbon dots obtained from the Rhododendron luteum (RL) plant in an environmentally friendly manner. The green RL were characterized using Fourier‐transform infrared spectroscopy (FTIR), UV‐Vis spectroscopy, transmission electron microscopy (TEM), and artificial intelligence (AI)‐based TEM analysis. The FTIR spectrum showed peaks corresponding to the hydroxyl (−OH) vibration of polyphenols at 3500 cm−1, the C=O vibration of cellulose derivatives at 1728 cm−1, and the C−O stretching of primary alcohol at 1041 cm−1. Two UV absorption peaks at roughly 253 nm (UV−C range), and 320 nm (UV−B range) were observed. The size of the green RL was measured to be less than 50 nm, and its morphology was characterized as dumbbell‐shaped through TEM analysis. In‐vitro studies were performed with HCT116 colon cancer, MCF‐7 breast cancer, and normal HUVEC cells. The results demonstrated that the RL‐CDs exhibited selective cytotoxic activity against HCT116 colon cancer cells. The results show that the RL extract stimulates cancer cell death by decreasing the CD44/24 ratio, and increasing apoptotic activity. These observations suggest that green RL‐CDs could be an effective anticancer agent in colon cancer therapy, investigating their potential in this direction could be a promising way for future research. |
| format | Article |
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| institution | Kabale University |
| issn | 2191-1363 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj-art-49df130a842e476fa724d52c9ea0e7112025-08-20T03:49:22ZengWiley-VCHChemistryOpen2191-13632025-05-01145n/an/a10.1002/open.202400303In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon DotsDr. Alper Durmaz0İbrahim Mizan Kahyaoğlu1Erdi Can Aytar2Ferdane Danışman Kalındemirtaş3Esra Sert4Ayşe Erol‐Bozkurt5Selcan Karakuş6Ali Nihat Gökyiğit Botanical Garden Application and Research Center Artvin Çoruh University 08000 Artvin TürkiyeDepartment of Chemistry Faculty of Science Ondokuz Mayıs University 55139 Samsun TürkiyeDepartment of Horticulture Faculty of Agriculture Usak University 64200 Usak TürkiyeDepartment of Physiology Faculty of Medicine Erzincan Binali Yildirim University 24100 Erzincan TürkiyeDepartment of Hematology Istanbul Faculty of Medicine Istanbul University 34093 Istanbul TürkiyeDepartment of Medical Biology Istanbul Faculty of Medicine Istanbul University 34093 Istanbul TürkiyeDepartment of Chemistry Faculty of Engineering Istanbul University-Cerrahpaşa 34320 Istanbul TürkiyeAbstract Colorectal cancer is the second most common cause of cancer‐related deaths worldwide and the third most common cancer overall. In this study, we investigate the anti‐colon cancer potential of phytochemically, and thermally synthesised novel green carbon dots based on Rhododendron luteum (RL‐CDs). A new synthesis method was used to produce carbon dots obtained from the Rhododendron luteum (RL) plant in an environmentally friendly manner. The green RL were characterized using Fourier‐transform infrared spectroscopy (FTIR), UV‐Vis spectroscopy, transmission electron microscopy (TEM), and artificial intelligence (AI)‐based TEM analysis. The FTIR spectrum showed peaks corresponding to the hydroxyl (−OH) vibration of polyphenols at 3500 cm−1, the C=O vibration of cellulose derivatives at 1728 cm−1, and the C−O stretching of primary alcohol at 1041 cm−1. Two UV absorption peaks at roughly 253 nm (UV−C range), and 320 nm (UV−B range) were observed. The size of the green RL was measured to be less than 50 nm, and its morphology was characterized as dumbbell‐shaped through TEM analysis. In‐vitro studies were performed with HCT116 colon cancer, MCF‐7 breast cancer, and normal HUVEC cells. The results demonstrated that the RL‐CDs exhibited selective cytotoxic activity against HCT116 colon cancer cells. The results show that the RL extract stimulates cancer cell death by decreasing the CD44/24 ratio, and increasing apoptotic activity. These observations suggest that green RL‐CDs could be an effective anticancer agent in colon cancer therapy, investigating their potential in this direction could be a promising way for future research.https://doi.org/10.1002/open.202400303AnticancerGreen nanostructureHCT116 colon cancer cellMCF-7 breast cancerRhododendron luteum |
| spellingShingle | Dr. Alper Durmaz İbrahim Mizan Kahyaoğlu Erdi Can Aytar Ferdane Danışman Kalındemirtaş Esra Sert Ayşe Erol‐Bozkurt Selcan Karakuş In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots ChemistryOpen Anticancer Green nanostructure HCT116 colon cancer cell MCF-7 breast cancer Rhododendron luteum |
| title | In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots |
| title_full | In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots |
| title_fullStr | In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots |
| title_full_unstemmed | In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots |
| title_short | In Vitro, Anti‐Colon Cancer Activity of Green Dumbbell‐Shaped Rhododendron luteum‐Based Carbon Dots |
| title_sort | in vitro anti colon cancer activity of green dumbbell shaped rhododendron luteum based carbon dots |
| topic | Anticancer Green nanostructure HCT116 colon cancer cell MCF-7 breast cancer Rhododendron luteum |
| url | https://doi.org/10.1002/open.202400303 |
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