Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast

In this study, a comparative approach was used with the aim of filling the knowledge gap regarding the productive and economic parameters of two native <i>B. mori</i> silkworm breeds (<i>Lines C</i> and <i>Z</i>) fed with different diets, namely mulberry leaves al...

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Main Authors: Mihaela Hăbeanu, Anca Gheorghe, Nicoleta Aurelia Lefter, Mihaela Dumitru, Smaranda Mariana Toma, Petru Alexandru Vlaicu, Teodor Mihalcea
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/16/5/482
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author Mihaela Hăbeanu
Anca Gheorghe
Nicoleta Aurelia Lefter
Mihaela Dumitru
Smaranda Mariana Toma
Petru Alexandru Vlaicu
Teodor Mihalcea
author_facet Mihaela Hăbeanu
Anca Gheorghe
Nicoleta Aurelia Lefter
Mihaela Dumitru
Smaranda Mariana Toma
Petru Alexandru Vlaicu
Teodor Mihalcea
author_sort Mihaela Hăbeanu
collection DOAJ
description In this study, a comparative approach was used with the aim of filling the knowledge gap regarding the productive and economic parameters of two native <i>B. mori</i> silkworm breeds (<i>Lines C</i> and <i>Z</i>) fed with different diets, namely mulberry leaves alone and mulberry leaves with <i>R. glutinis</i> yeast at two different concentrations (1 × 10<sup>9</sup> and 1 × 10<sup>7</sup> CFU/mL). The trifactorial experiment was carried out with 600 silkworms during the fifth instar, with 300 for each breed and 50 larvae per rearing tray. The larvae were randomly assigned into three feeding groups, with two replicates: in the control group (C), the larvae received ordinary mulberry leaves; in the first experimental group (RG-1), the larvae were given mulberry leaves supplemented with <i>R. glutinis</i> yeast at a 1 × 10<sup>7</sup> concentration; and in the second experimental group (RG-2), the larvae received the same quantity of mulberry leaves as the C and RG-1 groups, but with <i>R. glutinis</i> yeast at a 1 × 10<sup>9</sup> concentration. <i>Line C</i> larvae in the RG-1 group revealed higher values for most of the productive traits assessed. There was a positive and significant correlation between the weights and lengths of the larvae and the silk gland, the silk gland and the shell, the cocoon and the pupae and the shell, and the shell and the pupa size (<i>p</i> < 0.001). The steady decline in the parameters at a higher concentration suggests that <i>R. glutinis</i> positively influences a number of silkworm growth features at a recommended dosage of 1 × 10<sup>7</sup>; however, further research is required to more precisely identify the mechanisms involved.
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spelling doaj-art-49de3461d90746f48020fb8a5e28cbde2025-08-20T01:56:19ZengMDPI AGInsects2075-44502025-05-0116548210.3390/insects16050482Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> YeastMihaela Hăbeanu0Anca Gheorghe1Nicoleta Aurelia Lefter2Mihaela Dumitru3Smaranda Mariana Toma4Petru Alexandru Vlaicu5Teodor Mihalcea6Research Station for Sericulture Baneasa, 013685 Bucharest, RomaniaResearch Station for Sericulture Baneasa, 013685 Bucharest, RomaniaResearch Station for Sericulture Baneasa, 013685 Bucharest, RomaniaNational Research Development Institute for Animal Biology and Nutrition, Calea Bucuresti 1, 077015 Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, Calea Bucuresti 1, 077015 Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, Calea Bucuresti 1, 077015 Balotesti, RomaniaResearch Station for Sericulture Baneasa, 013685 Bucharest, RomaniaIn this study, a comparative approach was used with the aim of filling the knowledge gap regarding the productive and economic parameters of two native <i>B. mori</i> silkworm breeds (<i>Lines C</i> and <i>Z</i>) fed with different diets, namely mulberry leaves alone and mulberry leaves with <i>R. glutinis</i> yeast at two different concentrations (1 × 10<sup>9</sup> and 1 × 10<sup>7</sup> CFU/mL). The trifactorial experiment was carried out with 600 silkworms during the fifth instar, with 300 for each breed and 50 larvae per rearing tray. The larvae were randomly assigned into three feeding groups, with two replicates: in the control group (C), the larvae received ordinary mulberry leaves; in the first experimental group (RG-1), the larvae were given mulberry leaves supplemented with <i>R. glutinis</i> yeast at a 1 × 10<sup>7</sup> concentration; and in the second experimental group (RG-2), the larvae received the same quantity of mulberry leaves as the C and RG-1 groups, but with <i>R. glutinis</i> yeast at a 1 × 10<sup>9</sup> concentration. <i>Line C</i> larvae in the RG-1 group revealed higher values for most of the productive traits assessed. There was a positive and significant correlation between the weights and lengths of the larvae and the silk gland, the silk gland and the shell, the cocoon and the pupae and the shell, and the shell and the pupa size (<i>p</i> < 0.001). The steady decline in the parameters at a higher concentration suggests that <i>R. glutinis</i> positively influences a number of silkworm growth features at a recommended dosage of 1 × 10<sup>7</sup>; however, further research is required to more precisely identify the mechanisms involved.https://www.mdpi.com/2075-4450/16/5/482<i>R. glutinis</i>mulberry leavessilkwormlarvaecocoons
spellingShingle Mihaela Hăbeanu
Anca Gheorghe
Nicoleta Aurelia Lefter
Mihaela Dumitru
Smaranda Mariana Toma
Petru Alexandru Vlaicu
Teodor Mihalcea
Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
Insects
<i>R. glutinis</i>
mulberry leaves
silkworm
larvae
cocoons
title Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
title_full Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
title_fullStr Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
title_full_unstemmed Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
title_short Comparative Study of the Productive Parameters of Two Breeds of the <i>Bombyx mori</i> Silkworm Fed <i>Rhodotorula glutinis</i> Yeast
title_sort comparative study of the productive parameters of two breeds of the i bombyx mori i silkworm fed i rhodotorula glutinis i yeast
topic <i>R. glutinis</i>
mulberry leaves
silkworm
larvae
cocoons
url https://www.mdpi.com/2075-4450/16/5/482
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