Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L.
The transition of the shoot apical meristem from vegetative to reproductive phases is a critical stage in the plant life cycle. In contrast, the success of reproduction depends on the flowering initiation timing. One of the key repressors of flowering is the gene FLOWERING LOCUS C (FLC), which is im...
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University of Mazandaran
2024-09-01
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| Series: | Journal of Genetic Resources |
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| author | Parissa Jonoubi Farkhondeh Rezanejad Fatemeh Mahyapoor |
| author_facet | Parissa Jonoubi Farkhondeh Rezanejad Fatemeh Mahyapoor |
| author_sort | Parissa Jonoubi |
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| description | The transition of the shoot apical meristem from vegetative to reproductive phases is a critical stage in the plant life cycle. In contrast, the success of reproduction depends on the flowering initiation timing. One of the key repressors of flowering is the gene FLOWERING LOCUS C (FLC), which is important for determining flowering time. The present study, therefore, aimed to identify and investigate the expression patterns of Lepidium sativum genes, considering FLC. Specific primers were designed for the RT-PCR assays, amplifying a 361-nucleotide fragment of the LsFLC gene, recorded in the NCBI database under the accession number GenBank: KT582105.1. The sequenced fragment encoded for a deduced protein sequence of 120 amino acids. The expression of LsFLC in different plant organs was analyzed at various phenological stages, namely two days (early stage of vegetative development), 13 days (early vegetative stage), 28 days (vegetative maturity or transition stage), and during reproductive development stages, which fall on 33 days (early reproductive stage) and 34 days (flowering stage). Results showed that LsFLC expression levels were very high in the root and relatively high in leaves and stems, whereas no expression was allowed in flowers. Indeed, the mRNA levels were much higher during the vegetative phase compared to the transition to flowering, therefore agreeing with the known role of FLC as a promoter of vegetative growth and an inhibitor of flowering. Phylogenetic analysis showed that the LsFLC gene has a close relationship with Brassicaceae members, in particular with the highest homology with Arabidopsis lyrata. The current study has contributed to the knowledge of the functions of LsFLC proteins during the phenology of L. sativum and could contribute to strategies developed for improving this species in desirable traits. |
| format | Article |
| id | doaj-art-49d95039d1f543729f4330f4e947e51d |
| institution | Kabale University |
| issn | 2423-4257 2588-2589 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | University of Mazandaran |
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| series | Journal of Genetic Resources |
| spelling | doaj-art-49d95039d1f543729f4330f4e947e51d2025-01-27T06:11:46ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892024-09-0110214315110.22080/jgr.2024.27624.13985138Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L.Parissa Jonoubi0Farkhondeh Rezanejad1Fatemeh Mahyapoor2Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, IranThe transition of the shoot apical meristem from vegetative to reproductive phases is a critical stage in the plant life cycle. In contrast, the success of reproduction depends on the flowering initiation timing. One of the key repressors of flowering is the gene FLOWERING LOCUS C (FLC), which is important for determining flowering time. The present study, therefore, aimed to identify and investigate the expression patterns of Lepidium sativum genes, considering FLC. Specific primers were designed for the RT-PCR assays, amplifying a 361-nucleotide fragment of the LsFLC gene, recorded in the NCBI database under the accession number GenBank: KT582105.1. The sequenced fragment encoded for a deduced protein sequence of 120 amino acids. The expression of LsFLC in different plant organs was analyzed at various phenological stages, namely two days (early stage of vegetative development), 13 days (early vegetative stage), 28 days (vegetative maturity or transition stage), and during reproductive development stages, which fall on 33 days (early reproductive stage) and 34 days (flowering stage). Results showed that LsFLC expression levels were very high in the root and relatively high in leaves and stems, whereas no expression was allowed in flowers. Indeed, the mRNA levels were much higher during the vegetative phase compared to the transition to flowering, therefore agreeing with the known role of FLC as a promoter of vegetative growth and an inhibitor of flowering. Phylogenetic analysis showed that the LsFLC gene has a close relationship with Brassicaceae members, in particular with the highest homology with Arabidopsis lyrata. The current study has contributed to the knowledge of the functions of LsFLC proteins during the phenology of L. sativum and could contribute to strategies developed for improving this species in desirable traits.https://sc.journals.umz.ac.ir/article_5138_47d9b2cb2059aa5cf99465297e9fce58.pdfflowering repressorgarden cressgene expressionlsflc gene sequencingphylogenetic tree |
| spellingShingle | Parissa Jonoubi Farkhondeh Rezanejad Fatemeh Mahyapoor Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. Journal of Genetic Resources flowering repressor garden cress gene expression lsflc gene sequencing phylogenetic tree |
| title | Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. |
| title_full | Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. |
| title_fullStr | Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. |
| title_full_unstemmed | Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. |
| title_short | Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. |
| title_sort | gene expression analysis of deduced protein sequence from the flowering locus c flc homolog during vegetative and reproductive phases in lepidium sativum l |
| topic | flowering repressor garden cress gene expression lsflc gene sequencing phylogenetic tree |
| url | https://sc.journals.umz.ac.ir/article_5138_47d9b2cb2059aa5cf99465297e9fce58.pdf |
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