Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients
Periodontal diseases are localized chronic inflammatory conditions of the gingival and underlying bone and connective tissue. Platelet-activating factor (PAF), a potent inflammatory phospholipid mediator that has been previously detected in elevated levels in inflamed gingival tissues, in gingival c...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629350310001599666 |
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| _version_ | 1832558093470269440 |
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| author | Smaragdi Antonopoulou Alexandros Tsoupras George Baltas Helen Kotsifaki Zacharias Mantzavinos Constantinos A. Demopoulos |
| author_facet | Smaragdi Antonopoulou Alexandros Tsoupras George Baltas Helen Kotsifaki Zacharias Mantzavinos Constantinos A. Demopoulos |
| author_sort | Smaragdi Antonopoulou |
| collection | DOAJ |
| description | Periodontal diseases are localized chronic inflammatory conditions of the gingival and underlying bone and connective tissue. Platelet-activating factor (PAF), a potent inflammatory phospholipid mediator that has been previously detected in elevated levels in inflamed gingival tissues, in gingival crevicular fluid and in saliva, is implicated in periodontal disease. Our results from previous studies showed that the biologically active phospholipid detected in gingival crevicular fluid is a hydroxyl-PAF analogue. In this study, hydroxyl-PAF analogue was detected for the first time in human blood derived from patients with chronic periodontitis as well as from periodontally healthy volunteers. The hydroxyl-PAF analogue was purified by high-performance liquid chromatography, detected by biological assays and identified by electrospray analysis. In addition, the quantitative determination of PAF and hydroxyl-PAF analogue (expressed as PAF-like activity) showed a statistically significant increase in the ratio of hydroxyl-PAF analogue levels to PAF levels in periodontal patients, suggesting that this bioactive lipid may play a role in oral inflammation. |
| format | Article |
| id | doaj-art-49bd7ac7f490462b836caabb609a92f5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-49bd7ac7f490462b836caabb609a92f52025-02-03T01:33:15ZengWileyMediators of Inflammation0962-93511466-18612003-01-0112422122710.1080/09629350310001599666Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patientsSmaragdi Antonopoulou0Alexandros Tsoupras1George Baltas2Helen Kotsifaki3Zacharias Mantzavinos4Constantinos A. Demopoulos5Department of Science of Dietetics—Nutrition, Harokopio University, 70 El. Venizelou str., Athens 176 71, GreeceFaculty of Chemistry, National and Kapodistrian University of Athens, Panepistimioupolis, Athens, GreeceDepartment of Periodontology, National and Kapodistrian University of Athens, GreeceDepartment of Physiology, National and Kapodistrian University of Athens, GreeceDepartment of Periodontology, National and Kapodistrian University of Athens, GreeceFaculty of Chemistry, National and Kapodistrian University of Athens, Panepistimioupolis, Athens, GreecePeriodontal diseases are localized chronic inflammatory conditions of the gingival and underlying bone and connective tissue. Platelet-activating factor (PAF), a potent inflammatory phospholipid mediator that has been previously detected in elevated levels in inflamed gingival tissues, in gingival crevicular fluid and in saliva, is implicated in periodontal disease. Our results from previous studies showed that the biologically active phospholipid detected in gingival crevicular fluid is a hydroxyl-PAF analogue. In this study, hydroxyl-PAF analogue was detected for the first time in human blood derived from patients with chronic periodontitis as well as from periodontally healthy volunteers. The hydroxyl-PAF analogue was purified by high-performance liquid chromatography, detected by biological assays and identified by electrospray analysis. In addition, the quantitative determination of PAF and hydroxyl-PAF analogue (expressed as PAF-like activity) showed a statistically significant increase in the ratio of hydroxyl-PAF analogue levels to PAF levels in periodontal patients, suggesting that this bioactive lipid may play a role in oral inflammation.http://dx.doi.org/10.1080/09629350310001599666Platelet-activating factorGingival crevicular fluidPeriodontal diseaseElectrospray mass spectrometryPhospholipids. |
| spellingShingle | Smaragdi Antonopoulou Alexandros Tsoupras George Baltas Helen Kotsifaki Zacharias Mantzavinos Constantinos A. Demopoulos Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients Mediators of Inflammation Platelet-activating factor Gingival crevicular fluid Periodontal disease Electrospray mass spectrometry Phospholipids. |
| title | Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients |
| title_full | Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients |
| title_fullStr | Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients |
| title_full_unstemmed | Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients |
| title_short | Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients |
| title_sort | hydroxyl platelet activating factor exists in blood of healthy volunteers and periodontal patients |
| topic | Platelet-activating factor Gingival crevicular fluid Periodontal disease Electrospray mass spectrometry Phospholipids. |
| url | http://dx.doi.org/10.1080/09629350310001599666 |
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