Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process
In this study, we investigated the regulatory roles of the <i>juvenile hormone epoxide hydrolase</i> (<i>JHEH</i>) gene in the reproductive process of female <i>Macrobrachium nipponense</i>. Its total cDNA length was 1848 bp, encoding for 460 amino acids. It conta...
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2024-11-01
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| author | Jisheng Wang Mengying Zhang Hongtuo Fu Wenyi Zhang Yiwei Xiong Shubo Jin Hui Qiao Sufei Jiang |
| author_facet | Jisheng Wang Mengying Zhang Hongtuo Fu Wenyi Zhang Yiwei Xiong Shubo Jin Hui Qiao Sufei Jiang |
| author_sort | Jisheng Wang |
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| description | In this study, we investigated the regulatory roles of the <i>juvenile hormone epoxide hydrolase</i> (<i>JHEH</i>) gene in the reproductive process of female <i>Macrobrachium nipponense</i>. Its total cDNA length was 1848 bp, encoding for 460 amino acids. It contained conserved domains typical of epoxide hydrolases, such as the Abhydrolase family domain, the EHN epoxide hydrolase superfamily domain, and the “WWG” and “HGWP” motifs. The qPCR results showed that the expression of <i>Mn-JHEH</i> was the highest in hepatopancreas. <i>Mn-JHEH</i> was expressed at all stages of the embryonic and larval stages. The expression of <i>Mn-JHEH</i> at different developmental periods of the ovary was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. The RNA interference technique was used to study the role of <i>Mn-JHEH</i> in the process of ovarian maturation. The knockdown of <i>Mn-JHEH</i> with dsRNA in the experimental group resulted in a significant decrease in the percentage of ovaries exceeding stage O-III and the gonadal index compared with the control group. On day 14 (the second molt), the molt frequency was significantly higher in the control group than in the experimental group. The results showed that <i>Mn-JHEH</i> played an important role in ovarian maturation and molting. |
| format | Article |
| id | doaj-art-49b464e012d54f41a811d741d1812ebc |
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| issn | 1467-3037 1467-3045 |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-49b464e012d54f41a811d741d1812ebc2025-08-20T02:00:30ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014612134561347010.3390/cimb46120803Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive ProcessJisheng Wang0Mengying Zhang1Hongtuo Fu2Wenyi Zhang3Yiwei Xiong4Shubo Jin5Hui Qiao6Sufei Jiang7Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaIn this study, we investigated the regulatory roles of the <i>juvenile hormone epoxide hydrolase</i> (<i>JHEH</i>) gene in the reproductive process of female <i>Macrobrachium nipponense</i>. Its total cDNA length was 1848 bp, encoding for 460 amino acids. It contained conserved domains typical of epoxide hydrolases, such as the Abhydrolase family domain, the EHN epoxide hydrolase superfamily domain, and the “WWG” and “HGWP” motifs. The qPCR results showed that the expression of <i>Mn-JHEH</i> was the highest in hepatopancreas. <i>Mn-JHEH</i> was expressed at all stages of the embryonic and larval stages. The expression of <i>Mn-JHEH</i> at different developmental periods of the ovary was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. The RNA interference technique was used to study the role of <i>Mn-JHEH</i> in the process of ovarian maturation. The knockdown of <i>Mn-JHEH</i> with dsRNA in the experimental group resulted in a significant decrease in the percentage of ovaries exceeding stage O-III and the gonadal index compared with the control group. On day 14 (the second molt), the molt frequency was significantly higher in the control group than in the experimental group. The results showed that <i>Mn-JHEH</i> played an important role in ovarian maturation and molting.https://www.mdpi.com/1467-3045/46/12/803ovarian developmentmoltingendocrine hormoneenzyme JHEH |
| spellingShingle | Jisheng Wang Mengying Zhang Hongtuo Fu Wenyi Zhang Yiwei Xiong Shubo Jin Hui Qiao Sufei Jiang Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process Current Issues in Molecular Biology ovarian development molting endocrine hormone enzyme JHEH |
| title | Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process |
| title_full | Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process |
| title_fullStr | Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process |
| title_full_unstemmed | Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process |
| title_short | Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process |
| title_sort | regulation roles of juvenile hormone epoxide hydrolase gene 2 in the female river prawn em macrobrachium nipponense em reproductive process |
| topic | ovarian development molting endocrine hormone enzyme JHEH |
| url | https://www.mdpi.com/1467-3045/46/12/803 |
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