Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn <em>Macrobrachium nipponense</em> Reproductive Process
In this study, we investigated the regulatory roles of the <i>juvenile hormone epoxide hydrolase</i> (<i>JHEH</i>) gene in the reproductive process of female <i>Macrobrachium nipponense</i>. Its total cDNA length was 1848 bp, encoding for 460 amino acids. It conta...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/46/12/803 |
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| Summary: | In this study, we investigated the regulatory roles of the <i>juvenile hormone epoxide hydrolase</i> (<i>JHEH</i>) gene in the reproductive process of female <i>Macrobrachium nipponense</i>. Its total cDNA length was 1848 bp, encoding for 460 amino acids. It contained conserved domains typical of epoxide hydrolases, such as the Abhydrolase family domain, the EHN epoxide hydrolase superfamily domain, and the “WWG” and “HGWP” motifs. The qPCR results showed that the expression of <i>Mn-JHEH</i> was the highest in hepatopancreas. <i>Mn-JHEH</i> was expressed at all stages of the embryonic and larval stages. The expression of <i>Mn-JHEH</i> at different developmental periods of the ovary was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. The RNA interference technique was used to study the role of <i>Mn-JHEH</i> in the process of ovarian maturation. The knockdown of <i>Mn-JHEH</i> with dsRNA in the experimental group resulted in a significant decrease in the percentage of ovaries exceeding stage O-III and the gonadal index compared with the control group. On day 14 (the second molt), the molt frequency was significantly higher in the control group than in the experimental group. The results showed that <i>Mn-JHEH</i> played an important role in ovarian maturation and molting. |
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| ISSN: | 1467-3037 1467-3045 |