Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1.
In 2008, an unusual strain of methicillin-sensitive Staphylococcus aureus (MSSA68111), producing both Panton-Valentine leukocidin (PVL) and toxic shock syndrome toxin-1 (TSST-1), was isolated from a fatal case of necrotizing pneumonia. Because PVL/TSST-1 co-production in S. aureus is rare, we charac...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027246&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850127304560214016 |
|---|---|
| author | Zhi Li Dennis L Stevens Stephanie M Hamilton Tanyalak Parimon Yongsheng Ma Angela M Kearns Richard W Ellis Amy E Bryant |
| author_facet | Zhi Li Dennis L Stevens Stephanie M Hamilton Tanyalak Parimon Yongsheng Ma Angela M Kearns Richard W Ellis Amy E Bryant |
| author_sort | Zhi Li |
| collection | DOAJ |
| description | In 2008, an unusual strain of methicillin-sensitive Staphylococcus aureus (MSSA68111), producing both Panton-Valentine leukocidin (PVL) and toxic shock syndrome toxin-1 (TSST-1), was isolated from a fatal case of necrotizing pneumonia. Because PVL/TSST-1 co-production in S. aureus is rare, we characterized the molecular organization of these toxin genes in strain 68111. MSSA68111 carries the PVL genes within a novel temperate prophage we call ФPVLv68111 that is most similar, though not identical, to phage ФPVL--a phage type that is relatively rare worldwide. The TSST-1 gene (tst) in MSSA68111 is carried on a unique staphylococcal pathogenicity island (SaPI) we call SaPI68111. Features of SaPI68111 suggest it likely arose through multiple major recombination events with other known SaPIs. Both ФPVLv68111 and SaPI68111 are fully mobilizable and therefore transmissible to other strains. Taken together, these findings suggest that hypervirulent S. aureus have the potential to emerge worldwide. |
| format | Article |
| id | doaj-art-49b31a718fef4f169b8d81bf50b88b6f |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-49b31a718fef4f169b8d81bf50b88b6f2025-08-20T02:33:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2724610.1371/journal.pone.0027246Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1.Zhi LiDennis L StevensStephanie M HamiltonTanyalak ParimonYongsheng MaAngela M KearnsRichard W EllisAmy E BryantIn 2008, an unusual strain of methicillin-sensitive Staphylococcus aureus (MSSA68111), producing both Panton-Valentine leukocidin (PVL) and toxic shock syndrome toxin-1 (TSST-1), was isolated from a fatal case of necrotizing pneumonia. Because PVL/TSST-1 co-production in S. aureus is rare, we characterized the molecular organization of these toxin genes in strain 68111. MSSA68111 carries the PVL genes within a novel temperate prophage we call ФPVLv68111 that is most similar, though not identical, to phage ФPVL--a phage type that is relatively rare worldwide. The TSST-1 gene (tst) in MSSA68111 is carried on a unique staphylococcal pathogenicity island (SaPI) we call SaPI68111. Features of SaPI68111 suggest it likely arose through multiple major recombination events with other known SaPIs. Both ФPVLv68111 and SaPI68111 are fully mobilizable and therefore transmissible to other strains. Taken together, these findings suggest that hypervirulent S. aureus have the potential to emerge worldwide.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027246&type=printable |
| spellingShingle | Zhi Li Dennis L Stevens Stephanie M Hamilton Tanyalak Parimon Yongsheng Ma Angela M Kearns Richard W Ellis Amy E Bryant Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. PLoS ONE |
| title | Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. |
| title_full | Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. |
| title_fullStr | Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. |
| title_full_unstemmed | Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. |
| title_short | Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. |
| title_sort | fatal s aureus hemorrhagic pneumonia genetic analysis of a unique clinical isolate producing both pvl and tsst 1 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027246&type=printable |
| work_keys_str_mv | AT zhili fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT dennislstevens fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT stephaniemhamilton fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT tanyalakparimon fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT yongshengma fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT angelamkearns fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT richardwellis fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 AT amyebryant fatalsaureushemorrhagicpneumoniageneticanalysisofauniqueclinicalisolateproducingbothpvlandtsst1 |