Human eosinophil–airway smooth muscle cell interactions
Eosinophils are present throughout the airway wall of asthmatics. The nature of the interaction between human airway smooth muscle cells (ASMC) and eosinophils was investigated in this study. We demonstrated, using light microscopy, that freshly isolated eosinophils from healthy donors rapidly attac...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/096293500411550 |
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| _version_ | 1850104190252089344 |
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| author | J. Margaret Hughes Craig A. Arthur Shane Baracho Stephen M. Carlin Kristen M. Hawker Peter R.A. Johnson Carol L. Armour |
| author_facet | J. Margaret Hughes Craig A. Arthur Shane Baracho Stephen M. Carlin Kristen M. Hawker Peter R.A. Johnson Carol L. Armour |
| author_sort | J. Margaret Hughes |
| collection | DOAJ |
| description | Eosinophils are present throughout the airway wall of asthmatics. The nature of the interaction between human airway smooth muscle cells (ASMC) and eosinophils was investigated in this study. We demonstrated, using light microscopy, that freshly isolated eosinophils from healthy donors rapidly attach to ASMC in vitro. Numbers of attached eosinophils were highest at 2 h, falling to 50% of maximum by 20 h. Eosinophil attachment at 2 h was reduced to 72% of control by anti-VCAM-1, and to 74% at 20 h by anti-ICAM-1. Pre-treatment of ASMC for 24 h with TNF-α, 10 nM, significantly increased eosinophil adhesion to 149 and 157% of control after 2 and 20 h. These results provide evidence that eosinophil interactions with ASMC involve VCAM-1 and ICAM-1 and are modulated by TNF-α. |
| format | Article |
| id | doaj-art-49a53dabedab495f82c71781eb993d85 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-49a53dabedab495f82c71781eb993d852025-08-20T02:39:22ZengWileyMediators of Inflammation0962-93511466-18612000-01-0192939910.1080/096293500411550Human eosinophil–airway smooth muscle cell interactionsJ. Margaret Hughes0Craig A. Arthur1Shane Baracho2Stephen M. Carlin3Kristen M. Hawker4Peter R.A. Johnson5Carol L. Armour6Respiratory Research Group, Faculty of Pharmacy, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Faculty of Pharmacy, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Faculty of Pharmacy, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Department of Pharmacology, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Department of Pharmacology, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Department of Pharmacology, University of Sydney, Sydney NSW 2006, AustraliaRespiratory Research Group, Faculty of Pharmacy, University of Sydney, Sydney NSW 2006, AustraliaEosinophils are present throughout the airway wall of asthmatics. The nature of the interaction between human airway smooth muscle cells (ASMC) and eosinophils was investigated in this study. We demonstrated, using light microscopy, that freshly isolated eosinophils from healthy donors rapidly attach to ASMC in vitro. Numbers of attached eosinophils were highest at 2 h, falling to 50% of maximum by 20 h. Eosinophil attachment at 2 h was reduced to 72% of control by anti-VCAM-1, and to 74% at 20 h by anti-ICAM-1. Pre-treatment of ASMC for 24 h with TNF-α, 10 nM, significantly increased eosinophil adhesion to 149 and 157% of control after 2 and 20 h. These results provide evidence that eosinophil interactions with ASMC involve VCAM-1 and ICAM-1 and are modulated by TNF-α.http://dx.doi.org/10.1080/096293500411550 |
| spellingShingle | J. Margaret Hughes Craig A. Arthur Shane Baracho Stephen M. Carlin Kristen M. Hawker Peter R.A. Johnson Carol L. Armour Human eosinophil–airway smooth muscle cell interactions Mediators of Inflammation |
| title | Human eosinophil–airway smooth muscle cell interactions |
| title_full | Human eosinophil–airway smooth muscle cell interactions |
| title_fullStr | Human eosinophil–airway smooth muscle cell interactions |
| title_full_unstemmed | Human eosinophil–airway smooth muscle cell interactions |
| title_short | Human eosinophil–airway smooth muscle cell interactions |
| title_sort | human eosinophil airway smooth muscle cell interactions |
| url | http://dx.doi.org/10.1080/096293500411550 |
| work_keys_str_mv | AT jmargarethughes humaneosinophilairwaysmoothmusclecellinteractions AT craigaarthur humaneosinophilairwaysmoothmusclecellinteractions AT shanebaracho humaneosinophilairwaysmoothmusclecellinteractions AT stephenmcarlin humaneosinophilairwaysmoothmusclecellinteractions AT kristenmhawker humaneosinophilairwaysmoothmusclecellinteractions AT peterrajohnson humaneosinophilairwaysmoothmusclecellinteractions AT carollarmour humaneosinophilairwaysmoothmusclecellinteractions |