Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria
In this study, the bacterium Bacillus licheniformis has been isolated from oil reservoir; the ability of this bacterium to produce a biosurfactant was detected. Surface properties of the produced biosurfactant were confirmed by determining the emulsification power as well as surface and interfacial...
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| Format: | Article |
| Language: | English |
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Egyptian Petroleum Research Institute
2015-06-01
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| Series: | Egyptian Journal of Petroleum |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S111006211500029X |
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| author | H.S. El-Sheshtawy I. Aiad M.E. Osman A.A. Abo-ELnasr A.S. Kobisy |
| author_facet | H.S. El-Sheshtawy I. Aiad M.E. Osman A.A. Abo-ELnasr A.S. Kobisy |
| author_sort | H.S. El-Sheshtawy |
| collection | DOAJ |
| description | In this study, the bacterium Bacillus licheniformis has been isolated from oil reservoir; the ability of this bacterium to produce a biosurfactant was detected. Surface properties of the produced biosurfactant were confirmed by determining the emulsification power as well as surface and interfacial tension. The crude biosurfactant has been extracted from supernatant culture growth, and the yield of crude biosurfactant was about 1 g/l. Also, chemical structure of the produced biosurfactant was confirmed using FTIR analysis. Results revealed that, the emulsification power has been increased up to 96% and the surface tension decreased from 72 of distilled water to 36 mN/m after 72 h of incubation. The potential application of this bacterial species in microbial-enhanced oil recovery (MEOR) was investigated. The percent of oil recovery was 16.6% upon application in a sand pack column designed to stimulate an oil recovery. It also showed antimicrobial activity against the growth of different strains of SRB (sulfate reducing bacteria). Results revealed that a complete inhibition of SRB growth using 1.0% crude biosurfactant is achieved after 3 h. |
| format | Article |
| id | doaj-art-499587c16d924f4fb08a072aa90a437f |
| institution | Kabale University |
| issn | 1110-0621 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Egyptian Petroleum Research Institute |
| record_format | Article |
| series | Egyptian Journal of Petroleum |
| spelling | doaj-art-499587c16d924f4fb08a072aa90a437f2025-08-20T03:36:35ZengEgyptian Petroleum Research InstituteEgyptian Journal of Petroleum1110-06212015-06-0124215516210.1016/j.ejpe.2015.05.005Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteriaH.S. El-Sheshtawy0I. Aiad1M.E. Osman2A.A. Abo-ELnasr3A.S. Kobisy4Egyptian Petroleum Research Institute (EPRI), Nasr-City, Cairo, EgyptEgyptian Petroleum Research Institute (EPRI), Nasr-City, Cairo, EgyptDepartment of Botany and Microbiology, Faculty of Science, Helwan University, Cairo, EgyptDepartment of Botany and Microbiology, Faculty of Science, Helwan University, Cairo, EgyptEgyptian Petroleum Research Institute (EPRI), Nasr-City, Cairo, EgyptIn this study, the bacterium Bacillus licheniformis has been isolated from oil reservoir; the ability of this bacterium to produce a biosurfactant was detected. Surface properties of the produced biosurfactant were confirmed by determining the emulsification power as well as surface and interfacial tension. The crude biosurfactant has been extracted from supernatant culture growth, and the yield of crude biosurfactant was about 1 g/l. Also, chemical structure of the produced biosurfactant was confirmed using FTIR analysis. Results revealed that, the emulsification power has been increased up to 96% and the surface tension decreased from 72 of distilled water to 36 mN/m after 72 h of incubation. The potential application of this bacterial species in microbial-enhanced oil recovery (MEOR) was investigated. The percent of oil recovery was 16.6% upon application in a sand pack column designed to stimulate an oil recovery. It also showed antimicrobial activity against the growth of different strains of SRB (sulfate reducing bacteria). Results revealed that a complete inhibition of SRB growth using 1.0% crude biosurfactant is achieved after 3 h.http://www.sciencedirect.com/science/article/pii/S111006211500029XBiosurfactantsBacillus licheniformisMEORSulfate reducing bacteria |
| spellingShingle | H.S. El-Sheshtawy I. Aiad M.E. Osman A.A. Abo-ELnasr A.S. Kobisy Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria Egyptian Journal of Petroleum Biosurfactants Bacillus licheniformis MEOR Sulfate reducing bacteria |
| title | Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| title_full | Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| title_fullStr | Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| title_full_unstemmed | Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| title_short | Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| title_sort | production of biosurfactant from bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria |
| topic | Biosurfactants Bacillus licheniformis MEOR Sulfate reducing bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S111006211500029X |
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